Effect of TRPC6 knock-out on the mouse neuroinflammation induced by MPTP / 中国比较医学杂志

Objective To measure the level of microglia TRPC6 in mouse MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced neuroinflammation model and investigate its role in cytokine production and dopaminergic neuron damages. Methods Microglia were sorted by magnetic beads labeled with CD11b antibody and the level of TRPC6 in MPTP-induced neuroinflammation models was measured by western blotting. The proliferation of microglia and damages of dopaminergic neurons induced by MPTP were analyzed by immunofluorescence in CD11b-TRPC6 -/ - mice. Meanwhile, the expression of cryαB and cytokines in microglia was measured by western blotting and real-time quantitative PCR, respectively. Results The level of microglia TRPC6 in MPTP-induced neuroinflammation model was up-regulated. The expression of cryαB was increased and the cytokine level was down-regulated in the microglia in MPTP-injected CD11b-TRPC6 -/ - mice. Moreover, the dopaminergic neuron survival was improved in the MPTP-induced neuroinflammation model after TRPC6 knock-out in the microglia. Conclusions The expression of TRPC6 in microglia is up-regulated after MPTP injection, while in CD11b-TRPC6 -/ - mice the MPTP-induced cytokine expression is reduced, contributing to the improvement of dopaminergic neuron survival.

The relationship between abdominal fat volume and obstructive sleep apnea hypopnea syndrome in obesity people / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the relationship between abdominal fat volume and obstructive sleep apnea hypopnea syndrome in obesity people.</p><p><b>METHODS</b>From July 2009 to July 2010, 50 patients with BMI > 25 were prospectively selected for study from the patients who complained of snoring in the Respiratory department. The patients were divided into OSAHS group and non-OSAHS group according to the result of sleep apnea monitoring. All the patients also received full abdominal CT and the whole abdominal fat volume was measured by 3-D CT reconstruction system. SPSS 13.0 was used for statistical analysis.</p><p><b>RESULTS</b>The whole abdominal fat volume in the two groups was analyzed by T- test, which was significantly different between the two groups (P < 0.01). It showed that there was a statistical relationship between OSAHS and abdominal fat in obesity people.</p><p><b>CONCLUSION</b>In obesity people, OSAHS has a close relationship with abdominal fat volume. The abdominal fat volume is markedly higher in OSAHS patients than that in non-OSAHS people.</p>

Beneficial effects of liver X receptor agonist on adipose-derived mesenchymal stem cells transplantation in mice with myocardial infarction / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the effects of liver X receptor (LXR) agonist on adipose-derived mesenchymal stem cells (AD-MSCs) implantation into infarcted hearts of mice.</p><p><b>METHOD</b>AD-MSC(Fluc+) which stably expressed firefly luciferase (Fluc) were isolated from β-actin-Fluc transgenic mice and characterized by flow cytometry. Male FVB mice were randomly allocated into the following four groups (n = 10 each): (1) sham group; (2) MI + PBS group; (3) MI + AD-MSC(Fluc+) group; (4) MI + AD-MSC(Fluc+) + LXR agonist (T0901317) group. AD-MSC(Fluc+) or PBS were injected intramyocardial into peri-infarcted region of mice heart after permanent left anterior descending (LAD) artery ligation. Bioluminescence imaging (BLI) was performed for quantification of injected cells retention and survival. Cardiac function was evaluated by echocardiography.</p><p><b>RESULTS</b>The AD-MSC(Fluc+) were positive for CD44 and CD90 by flow cytometry. BLI evidenced the firefly luciferase expression of AD-MSC(Fluc+) which was positively correlated with cell numbers (r(2) = 0.98). The results of BLI in vivo revealed that LXR agonist could improve the survival of AD-MSC(Fluc+) at day 7, 14 and 21 after transplantation compared with AD-MSC(Fluc+) alone group. Cardiac function was further improved in combination therapy group compared with AD-MSC(Fluc+) alone group (P < 0.05).</p><p><b>CONCLUSIONS</b>LXR agonist T0901317 can improve the retention and survival of intramyocardial injected AD-MSC(Fluc+) post-MI, and the combination therapy of T0901317 and AD-MSC(Fluc+) has a synergetic effect on improving cardiac function in this model.</p>

Expression of heterogeneous nuclear ribonucleoprotein K in hepatocellular carcinoma tissues and cell line of different cell densities / 第二军医大学学报

Objective: To investigate the expression changes and the subcellular localization of heterogeneous nuclear ribonucleoprotein K (hnRNP K) in hepatocellular carcinoma (HCC) and cell line of different cell densities. Methods: The expression of hnRNP K in HCC was detected by immunohistochemical staining. The differential expression of hnRNP K in the HCC tissue (n = 30) and paired paracancerous tissue was identified by Western blotting assay. The expression and sub-cellular location of hnRNP K in HCC cell line of different cell densities were determined by immunocytochemical staining and Western blotting assay. Results: Expression of hnRNP K was mainly detected in the nucleus of HCC cells. Twenty-one of the 30 hepatic cancer tissues had higher hnRNP K expression than the paired paracancerous tissues. Sixteen of the 20 HCC samples with HBV infection had an increased expression of hnRNP K; in the 10 HCC samples without HBV infection, 5 had an increased expression of hnRNP K. Immunocytochemical staining and Western blotting assay both showed that the expression of hnRNP K was in creased in the nucleus of HCC cells with the increase of cell densities (30%, 50% and 90% confluence); the result of Western blotting assay also showed that there was no obvious change in hnRNP K expression in the cytoplasm of HCC cells of different cell densities(30%, 50%, 70% and 90% confluence). Conclusion: The expression of hnRNP K is elevated in HCC tissues and has an increasing tendency in the nucleus with the increase of cell density. Infection of HBV may be associated with the up-regulation of hnRNP K in HCC cells.

Involvement of TRAIL up-regulation of CD4+, CD8+ T cells in liver injury in chronic hepatitis B / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To describe a novel mechanism for TRAIL up-regulation of CD4+, CD8+ T cells to participate in the pathophysiological process in patients with chronic hepatitis B (CHB).</p><p><b>METHODS</b>The serum levels of soluble TRAIL (sTRAIL), IFN-gamma and membrane bound TRAIL expression on peripheral leucocytes from 58 CHB patients were examined by ELISA and flow cytometry respectively. The levels of TRAIL were compared with the baseline levels of 15 healthy controls, and correlation analysis were performed between ALT, TBil and PT, morphological change in hepatic tissues.</p><p><b>RESULTS</b>The results showed that TRAIL levels on membranes of CD4+, CD8+ T cells in CHB patients were much higher than the healthy controls (P < 0.001), which of CD4+ T cells positively correlated with serum TBil (r=0.354, P = 0.008), Serum IFN-gamma level (r=0.302, P = 0.011) and which of CD8+ T cells positively correlated with serum TBil (r=0.522, P = 0.000), ALT (r=0.393, P = 0.003), PT (r=0.385, P = 0.004), serum IFN-gamma level (r=0.307, P = 0.009). The serum levels of soluble TRAIL only correlated with serum HBeAg expression (r=0.695, P = 0.001).</p><p><b>CONCLUSION</b>These findings suggest that the expression of TRAIL on the membranes of lymphocytes was up-regulated, which may take part in the immunopathogenesis in CHB patients. TRAIL expression can be induced either by virus-specific protein expression or by inflammation cytokine IFN-gamma</p>