ABSTRACT
<p><b>OBJECTIVE</b>To observe the bone specimen of SANFH (steroid-induced avascular necrosis of femoral head) and the effect of Gufuhuo decoction on local apoptosis of SANFH.</p><p><b>METHOD</b>Model rabbits of necrosis of femoral head, made by intragluteal injection with prednisone acetate, were killed after 6 weeks to study the apoptosis by means of TUNEL.</p><p><b>RESULT</b>A great number of faded osteocytes and osteoblasts existed in specimen of SANFH. There was significant difference in apoptosis indexes between model and control group, Gufuhuo decoction and Xianlinggubao group (P < 0.05).</p><p><b>CONCLUSION</b>In the course of SANFH, the necrosis results from the interaction of apoptosis and necrosis of steroid-induced osteocytes and osteoblasts. Gufuhuo decoction has some effects on improving apoptosis and necrosis of SANFH.</p>
Subject(s)
Animals , Female , Male , Rabbits , Apoptosis , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Femur Head Necrosis , Pathology , In Situ Nick-End Labeling , Leeches , Chemistry , Materia Medica , Pharmacology , Osteoblasts , Pathology , Osteocytes , Pathology , Plants, Medicinal , Chemistry , Prednisone , Random AllocationABSTRACT
<p><b>OBJECTIVE</b>To probe the of Gufuhuotang effect on hemorheology and lipid metabolism of hormonal necrosis of femoral head.</p><p><b>METHOD</b>32 male rabbits of New Zealand were divided at random into pathological pattern group, normal control group, low-dosage of Gufuhuotang group and high-dosage group; models of osteocyte necrosis of head of femur were made with intragluteal injection of hydroprednisone acetate; the rabbits were observed weekly and killed 6 weeks later and HE pathological section was made to observe hemorheology index and blood-liquid content, and then analysis and comparison were made.</p><p><b>RESULT</b>Gufuhuotang could obviously decrease total blood low-shear viscosity, plasma viscosity, hemacocrit, serum cholesterol and triglyceride contents; under light microscope empty bone lacunas of femoral head were less, fat cells of medullary cavity were less and relatively small, and bone trabecula was sparse with no break.</p><p><b>CONCLUSION</b>Gufuhuotang has a distinct preventive and curative effect on rabbits' hormonal necrosis of femoral head.</p>