Detection and identification of a new bat bocavirus species in Yunnan / 军事医学

Objective To discover more novel bat viruses and molecularly characterize bat-borne bocavirus diversity in Yunnan.Methods Twenty-six Aselliscus stoliczkanus were sampled in Jinghong , Yunnan, and subjected to viral metagenomic analysis.Specific PCR was used to detect any bocavirus in these samples based on the metagenomic result , while full genome was amplified and compared with other bocaviruses .Results and Conclusion Totally, 3 of the 26 (11.5%) bats were positive for bocavirus, the full genome of which contained 5203 nucleotides and could encode NS1, NP and VP1/VP2 proteins.Phylogenetic analysis showed that this virus shared up to 58.7% and 53.3% amino acid identities with canine bocavirus 1 and canine minute virus .According to ICTV criteria (85%amino acid sequence identity ) on a new species of bocavirus , this virus could be a novel species within genus Bocaparvovirus .This study provides important data to better understand viral diversity in bats and to uncover the relationship between bocavirus and its hosts .

Clinical efficacy analysis of adult sinonasal rhabdomyosarcoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the clinical prognostic impact factors of adult sinonasal rhabdomyosarcoma (SNRMS).@*METHOD@#The clinical features, treatment methods, and disease outcome were reviewed retrospectively for twenty-three adult SNRMS between 2006 January and 2014 December. The survival analysis was performed by Kaplan-Meier estimate and the comparison between groups by Log-rank test. Multivariate analysis was carried out by Cox proportional hazard model.@*RESULT@#Patients' ages ranged from 18 to 59 years (median, 23.2 years). With a median follow-up of 20 moths (3-47 moths), 14 cases dead and 9 cases alive, the 1-year and 2-year overall survival (OS) rates were 77.1% and 35.0%, respectively. Within the 1-year and 2-year OS rates,early stage group had a higher overall survival rates than advanced diease group (100.0%, 66.7% and 83.3%, 10.5%, P < 0.05); combined therapy group had a higher overall survival rates than single treatment group (86.7%, 50.0% and 50.8%, 0, P < 0.05). In the non-metastasis group (21 cases), 1-year and 2-year distant metastasis rates were 38.1% and 70.5%, respectively. Multivariate analysis showed that radiotherapy, chemotherapy and tumor diameter less than 5 cm were good prognostic factors (P < 0.05), while the lymph node metastasis, meningeal involvement and orbital involvement were poor prognostic factors (P < 0 05). In the 14 cases of dead patients, 92 8% (13/14) died of distant metastasis.@*CONCLUSION@#Adult RMS had a high advanced rate with poor prognosis. Distant metastasis is the leading cause of death. Controlling distant metastasis is a key to improve the survival rate.

The analysis of surgery effect of OSAHS children accompanied with sinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To discuss the clinical efficacy of adenoidectomy and tonsillectomy for the OSAHS children with sinusitis.@*METHOD@#Reviewing 112 cases of children with OSAHS, in which the 80 patients without chronic sinusitis, 32 ones with chronic sinusitis. Among them, 103 cases of children with adenoidectomy, tonsil resection, 9 cases adenoidectomy only. We did questionnaire survey before and after surgeryand also did post-surgery clinical follow-up.@*RESULT@#The effective rate was 90.0% for the OSAHS children without sinusitis after adenoid and tonsil ectomy surgery, while the effective rate was 46.9% for the ones with sinusitis, the efficiency was significant different in the two groups after surgery; the 16.1% children after tonsil ectomy surgery complained the incidence of pharyngeal foreign body sensation and pharyngeal lymphoid hyperplasia in children was 23.2%.@*CONCLUSION@#The efficiency was aviable for the OSAHS children without sinusitis after surgery; The efficiency was not aviable enough for the OSAHS children with sinusitis after surgery, so the latter one need adjuvant drug treatment; We also should be attention to the pharyngeal foreign body sensation and the lymphoid hyperplasia in children after tonsillectomy.

Retrospective analysis of upper aerodigestive tract cancer and thyroid cancer / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the definition, etiology, diagnosis, treatment and prognosis in patients with multiple primary malignant tumor of upper aerodigestive tract cancer and thyroid cancer.@*METHOD@#Retrospectively analyze the events of 7 cases with multiple primary malignant tumors of upper aerodigestive tract cancer and thyroid cancer.@*RESULT@#Lesions of upper aerodigestive tract cancer and thyroid cancer located on the same side. After 4 to 8 years of follow-up, 2 cases of hypopharyngeal cancer and thyroid cancer got poor prognosis, prognosis of other patients with early stage laryngeal cancer and thyroid cancer is better.@*CONCLUSION@#The prognosis of multiple primary malignant tumors in patients with upper aerodigestive tract cancer and thyroid cancer depends on the biological behavior of aerodigestive tract cancer.

Preliminary study on treatment of lingual tonsil hypertrophy by endoscopic assisted coblation / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the feasibility and safety of lingual tonsil excision by endoscopic assisted coblation.@*METHOD@#Twenty seven cases with lingual tonsil hypertrophy were recruited in this study. Preoperative fibrolaryngoscope and CT and (or) MRI examination of the base of the tongue, lingual tonsil was removed by 70 degrees nasal endoscopy-assisted plasma radiofrequency after nasotracheal intubation. The tongue wound healing and post-operative bleeding were observed after operation, the advantages of the endoscopic-assisted plasma radiofrequency on lingual tonsil removal were analyzed, and its feasibility and safety were summarized.@*RESULT@#During the surgery the base of the tongue and epiglottis had satisfaction exposure in the same operative field. No complications happened in the surgery and endotracheal tube was plucked safely after operation. There was no primary bleeding, and the number of secondary bleeding were 4 cases, accounting for 14.81% (4/27), respectively occurred on the sixth, seventh, tenth and twelfth day with the amount of bleeding of 30-70 ml. The bleeding were cured after conservative treatment or re-application applicating of radiofrequency; 27 patients had no taste disturbance, and they were followed up for 6-30 months without recurrence.@*CONCLUSION@#Lingual tonsil excision by nasal endoscopic-assisted coblation has enough exposure, complete resection and low recurrence rate. Stopping bleeding should be attentioned to prevent postoperative bleeding.

Analysis of 13 cases of low-pressure laparoscopic appendectomy during pregnancy / 中国综合临床

Objective To investigate the value of low-pressure laparoscopic technique for appendicitis surgery during early and mid-term pregnancy.Methods The clinical data of 13 pregnant women underwent low-pressure laparoscopic appendectomy from July 2007 to December 2010 were analyzed retrospectively.Results All the 13 cases were performed laparoscopic appendectomy successfully without laparotomy and cured completely before discharged from hospital No intestinal fistula,wound infection,abortion and other complications occurred.During follow-up,9 cases of full-term pregnancy patients gave birth to healthy babies,and there was no abortion and premature delivery on 4 patients still in their pregnancy.Conclusion For women at early or mid-term pregnancy with acute appendicitis,low-pressure laparoscopic appendectomy could reduce the stimulus of surgical operation on uterus and decrease the risk of abortion.

The modified rhinotomy for treatment of tumors involving skull base / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To introduction the clinical application and surgical technique of the modified rhinotomy for treatment of skull base neoplasms.@*METHOD@#The diagnosis and surgical outcome of 20 patients with skull base neoplasms were reviewed retrospectively.@*RESULT@#All the cases were involvement with nasal cavity, ethmoidal sinus, sphenoidal sinus and medial wall of orbit. Two cases were involvement with pterygopalatine fossa, infratemporal fossa and intra calvarium separately. All cases were treated by modified rhinotomy. Among that 2 patient were with juvenal angiofibroma treated by combined with maxillary transposition and pterional and zygomatic approach, and 2 cases were with craniofacial approach. No complications were found relating to surgery. 6 patients was recurrent during follow-up among 6 to 60 months.@*CONCLUSION@#The modified rhinotomy is a effective and safe surgical approach to treat skull base neoplasms located in nasal cavity, ethmoidal sinus and sphenoidal sinus. Tumor involved with pterygopalatine fossa, inferotemporal fossa and intracranial extension, other surgical approach should be used at the same time.

Clinical significance and relationship between HIF-1alpha, COX-2, VEGF and microvessel density expression in laryngeal carcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the role of HIF-1alpha, COX-2, VEGF proteins and Microvessel density in angiogenesis and metastasis of laryngeal carcinoma.@*METHOD@#Surgically resected specimens of 64 laryngeal carcinomas were studied by immunohistochemical staining for HIF-1alpha protein, COX-2 protein, VEGF and MVD.@*RESULT@#Positive expressions of HIF-1alpha, COX-2 protein and VEGF were found in 65.63%, 68.75% and 71.88% of laryngeal carcinoma specimens, respectively. The mean MVD in this series was 35.79 +/- 9.49. The expression of HIF-1alpha, COX-2 and VEGF positively correlated with MVD expression. There was a positive relationship between HIF-1alpha, COX-2 and VEGF expression. The microvessel density (MVD) in HIF-1alpha, COX-2 and VEGF positive tumors was significantly higher in patients with cervical lymph node metastases than that in patients without metastases.@*CONCLUSION@#HIF-1alpha, COX-2 and VEGF proteins may play an important role in the angiogenesis of laryngeal carcinoma. HIF-1alpha regulate the expression and function of its downstream target genes (COX-2 and VEGF), which play a critical role in the angiogenesis of laryngeal carcinoma, metastases in the process of laryngeal cancer cell spreading to cervical lymph nodes.

Research of cellular toxic effect to Hep-2 of recombinant toxin MSH-Ang / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the cytotoxicity of recombinate toxin MSH-Ang to Hep-2.@*METHOD@#The depurated MSH-Ang were applied in cytotoxicity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep-2 were observed.@*RESULT@#Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing.@*CONCLUSION@#Recombinant toxin MSH-Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.

The treatment of fibrous dysplasia involving skull base / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the diagnosis and treatment of fibrous dysplasia (FD) involving the skull base.@*METHOD@#The clinical data of 15 patients with fibrous dysplasia involving the skull base was retrospective analysis.@*RESULT@#All patients were underwent CT examinations. FD involved orbital roof in 15 patients, ethmoidal bone in 15 patients, middle and lower nasal turbinate in 8 patients, frontal bone in 8 patients, sphenoidal bone in 6 patients, and maxillary bone in a patient. Although the optic canal was affected in 4 patients, only one patient had impaired vision. All the patients were treated by craniofacial approach. One patient with impaired vision was performed decompression of optic canal and had improved in vision. The titanium mesh was use for construction of skull base defect. There were not complications, such as infection, cerebral hemorrhage, etc.@*CONCLUSION@#It should be underwent surgical treatment for fibrous dysplasia involving skull base which resulted in clinical manifestations. Whether prophylactic decompression of optic canal is performed or notr depends on the patients clinical and radiological information.

Laryngeal cyst after partial laryngectomy / 中国耳鼻咽喉头颈外科

OBJECTIVE To study the incidence,pathogenesis,diagnosis and treatment of laryngeal cyst after partial laryngectomy.METHODS The clinical data of 228 cases of partial laryngectomy from January 2003 to June 2009 were retrospectively analyzed,and related literature was reviewed.RESULTS Two cases were found to have laryngeal cysts after partial laryngectomy during the post-operative follow-up.One occurred after 20 months,and the other occurred after 19 months.The cyst located on the tongue surface of the epiglottic was removed by CO2 laser under suspended laryngoscope,while the one located at the subglottic area was removed via cervical approach.So far,a total of 9 cases were reported.CONCLUSION Laryngeal cyst after partial laryngectomy is uncommon.That may be the mucosa planted during the operation.It could be misdiagnosed as recurrent laryngeal carcinoma.CT or MRI is helpful for diagnosis.Operation with CO2 laser under suspended laryngoscope or via cervical approach may be chosen.

Approach for benign parapharyngeal space tumors / 中国耳鼻咽喉头颈外科

OBJECTIVE To study the application of transcervical approach for benign parapharyngeal space tumors.METHODS The clinical data of 19 patients(20 operations) with benign parapharyngeal space tumors were analyzed retrospectively.RESULTS The 19 patients with benign parapharyngeal tumors were operated on in Tongren Hospital from July 2004 to July 2007.All patients were operated on through trans-cervical approach under general anesthesia.They were followed up from 6 to 36 months.Recurrence was found in a girl 15 months after operation,and was reoperated on by the same approach.No complications occurred.CONCLUSION The patients with parapharyngeal space tumors must be carefully studied by CT or/and MRI scan.If a clear tumor capsule is identified,the tumor can be removed through transcervical approach.Transcervical approach is a useful approach with least damage,and nearly all benign tumors of parapharyngeal space can be removed through it.