ABSTRACT
<p><b>OBJECTIVE</b>To compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.</p><p><b>METHODS</b>After hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.</p><p><b>RESULTS</b>Of 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.</p><p><b>CONCLUSION</b>DNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.</p>
Subject(s)
Humans , DNA Repair , Genetics , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Nasopharyngeal Neoplasms , Genetics , Pathology , Nasopharynx , Metabolism , Pathology , Oligonucleotide Array Sequence Analysis , Methods , RNA, Neoplasm , Genetics , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
AIM: To investigate the expression map of two p53 binding proteins 53BP1 and 53BP2 in nasopharyngeal carcinoma (NPC) tissue.METHODS: The expression of 53BP1 and 53BP2 mRNA in NPC biopsy and control group are tested by RT-PCR. The expression of two mRNA in NPC paraffin section are examined by in situ hybridization. RESULTS: No expression of 53BP1 mRNA was found in NPC tissue and control group. However, expression of 53BP2 was detected in NPC biopsy and control group by RT-PCR, specific expressoin found cancerous nest in NPC paraffin section by in situ hybridization.CONCLUSION: The high expression of 53BP2 may be related to the development of NPC.