ABSTRACT
BACKGROUND:The mechanical index is an important method for the evaluation of the therapeutic efficiency of drug treatment for osteoporosis animal models. OBJECTIVE: To explore the effects of various drug treatments on osteoporosis through a mechanical performance test about the femoral compression of rats. METHODS: Thirty-six Wistar female rats were randomized into six groups: normal control group, model group, Dan Qiparticles group, alpha-D3 group, premarin group, ipriflavone group, with six rats in each group. Osteoporosis models were made in al groups except for the normal control group, and after modeling, the rats in different groups were treated withDan Qi particles, alpha-D3 group, premarin and ipriflavone, respectively. After 15 weeks, the rats were kiled by abdominal aortic bloodletting to take out the left and right femurs that were placed on a universal testing machine to perform a compressive test at a speed of 5 mm/min. RESULTS AND CONCLUSION:The maximum load, maximum stress, maximum displacement, maximum strain, and elastic modulus were significantly lower in the model group than the other four groups (P 0.05). These findings indicate that osteoporosis leads to the variation of compression mechanical properties of the femur. There are good compression mechanical properties of the femur after treatment with premarin and ipriflavone, andDan Qi particles has the best effect.
ABSTRACT
Objective To establish the determination method of total polyphenols in vitis amurensis seed extract.Methods Using gallic acid as reference substance,the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry.Results There was a good linearity between gallic acid concentration and absorbance in the range 0.002-0.010 g?L-1(Y=86.243X+0.007,r=0.9996);the precision RSD was 0.14%;the stability RSD was 1.77%;the repeatability RSD was 1.44%;the average recovery rate was 102.00%(RSD=1.37%).Conclusion This method is simple,fast and has high sensitivity and good reproducibility.
ABSTRACT
Objective To study the protective effects of the polyphenols of vitis amurensis Rupr(PVAR) on oxidative damage in rats caused by chronic alcohol intakes.Methods Forty male Wistar rats were divided into 4 groups according rats' weights: normal control group,alcohol injury group(the rats were treated with 10 mL?kg-1 33% alcohol via i.g);PVAR group(after ingesting 200 and 400 mg?kg-1 PVAR,the male rats were treated with 10 mL?kg-1 33% alcohol via i.g).After 8 weeks,the concentrations of malondialdehyde(MDA),reactive oxygen species(ROS),glutathione(GSH) and the activities of SOD and heme oxygenase-1(HO-1) in rat liver tissues were measured.The activities of serum glutamic-pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) in rats were measured.Results Compared with alcohol injury group,the concentrations of MDA,ROS,GPT and GOT were decreased significantly(P