Commonly Seen Gene Polymorphisms in Inflammatory Bowel Disease / 胃肠病学

Inflammatory bowel disease(IBD),including ulcerative colitis(UC)and Crohn's disease(CD),is a group of chronic and relapsed inflammatory diseases of the intestinal tract. Genetic factors together with immunological, environmental and infectious factors contribute to the pathogenesis of IBD,however,the precise etiology is still uncertain. Susceptibility genes have great impact on the occurrence,development,clinical phenotypes,and even follow-up treatment of IBD,which indicates the significant roles of genetic factors in this disease. In this article,the well-studied and newly found genes related to IBD and their single nucleotide polymorphisms(SNPs)were reviewed.

Effects of antioxidant and NF-?B on the induction of IL-8 in human colon cancer cell line HT-29 cells in vitro / 中国病理生理杂志

AIM: To investigate the role of nuclear factor ?B (NF-?B) in the induction of IL-8 gene by TNF-? in colon cancer cells and the effect of antioxidant on the induction of IL-8. METHODS: ELISA was used to detect the concentrations of IL-8. IL-8 mRNA was analyzed by using RT-PCR. NF-?B in the cell nuclei was detected with electrophoretic mobility shift assay. RESULTS: (1) IL-8 production and IL-8 mRNA expression induced by TNF-? was blocked by pyrrolidine dithiocarbamate (PDTC). (2) TNF-? triggered the activation and translocation of NF-?B and PDTC inhibited the activation of NF-?B induced by TNF-?. CONCLUSION: The induction of IL-8 gene and protein by TNF-? is dependent on the activation of NF-?B. Antioxidants may inhibit the induction of IL-8 gene and protein through inhibiting NF-?B activation.

Relationship between uPA and NF-?B p65 expression and its clinical significance in breast cancer / 中国病理生理杂志

AIM: To evaluate the relationship between uPA and NF-?B p65 expression and its clinical significance in breast cancer. METHODS: The uPA mRNA was measured by real-time fluorescent quantitative PCR in 46 cases of breast cancer tissues and their adjacent counterparts. NF-?B p65 were measured using immunohistochemistry. RESULTS: The expression of uPA gene was elevated in 63% of cases, and there was a strong correlation between NF-?B p65 and uPA expression (r=0.451,P

Effect of dominant negative Ⅰ?B? transfection on NF-?B pathway and cell motility in breast cancer / 中国病理生理杂志

AIM:To observe the effect on NF-?B pathway and cell motility in breast cancer cell lines after transfection of dominant negative I?B? plasmid.METHODS:After stable transfection of mutant I?B? plasmid into highly metastatic breast cancer lines MDA-MB-231 and MDA-MB-435,we detected NF-?B binding activity by EMSA,cell growth ability by cell growth curve,colony forming test,and cell motility by millicell-PCF chamber.RESULTS:Constitutive activities in MDA-MB-231 and MDA-MB-435 were observed.Stable transfection of a dominant negative Ⅰ?B? resulted in downregulation of NF-?B binding activity,thus inhibited cell mobility without significant effect on cell growth.CONCLUSION:Cell migration ability is inhibited in highly invasive breast cancer cells by inhibition of NF-?B pathway in vitro.