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Chinese Journal of Biotechnology ; (12): 262-271, 2008.
Article in Chinese | WPRIM | ID: wpr-276129

ABSTRACT

In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.


Subject(s)
Culture Techniques , Methods , Drug Tolerance , Genetics , Flavones , Genetic Variation , Genetics , Plant Leaves , Genetics , Random Amplified Polymorphic DNA Technique , Salt-Tolerant Plants , Genetics , Seedlings , Genetics , Sodium Chloride , Pharmacology , Superoxide Dismutase , Taraxacum , Genetics
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