ABSTRACT
Aim To explore the mechanism of Fluspirilene inhibiting HCC through decreasing the expression of Akt.Methods The difference of mRNA was verified by the test of protein expression between Fluspirilenc treatment group and control group by HCC experiment in vivo and vitro, including Western blot, IHC after mRNA array.Results Akt expression was lower in Fluspirilene treatment group than that in control group by mRNA array.Protein expression of Akt, phosphorylate-CDK2 and phos- phorylate-Rb decreased massively in Fluspirilene treatment group in a concentration-dependent manner in HepG2 and Huh7 cells by Western blotting compared with those in control group.Declined expression of phosphorylate-Akt was proved in a concen- tration-dependent manner in xenograft tumor tissues in Fluspirilene treatment group compared with that in control group in IHC test.Conclusions Fluspirilene inhibits HCC by decreasing significantly the protein expression of Akt, phosphorylat-Akt, phos- phorylate-CDK2 and phosphorylate-Rb.
ABSTRACT
Purpose Manganese (Mn) concentration is one of the basic factors that have impact on manganese-enhanced magnetic resonance imaging (MEMRI) enhancement of neoplasm.This study aims to elucidate the impact of Mn concentration on enhancement of MEMRI in different colon cancer cells.Materials and Methods The human colon cancer cell SW620 with high metastatic potential,SW480 with low metastatic potential and the normal cell CCD841 CoN were performed MEMRI under different Mn concentrations (0,0.05,0.10 and 0.20 mmol/L) in vitro.The average values ofT1 shortening were calculated by T1 map scan in different cells under different Mn concentrations.Results When the concentration of Mn was 0.10 and 0.20 mmol/L,the average value of T1 shortening of the three cell lines was SW620＞SW480＞CCD841 CoN (P＜0.01).There was no significant difference in the average T1 value of the normal cell lines under different Mn concentrations (0.05,0.10,0.20 mmol/L) (P＞0.05).The average T1 value of two tumor cells increased with the increase of Mn concentration.Under the condition of high Mn concentration (0.10 and 0.20 mmol/L),SW620 took in more Mn than SW480.Conclusion Colon cancer cells increase Mn uptake with increasing of Mn concentration.MEMRI can distinguish colon cancer cells with different metastatic potential.