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OBJECTIVE: To establish HPLC method for the determination of the contents of atenolol, hydrochlorothiazide and nifedipine in xueyaan sustained release tablets. METHODS: The determination was performed on ZORBAX SB C18 column with column temperature at 35℃ . The mobile phase consisted of 0. 01mol? L- 1 sodium dihydrogen phosphate solution- methanol- acetonitrile ( 6∶ 3∶ 1) . The UV detection wavelength was set at 272nm and the sample size was 20? L. RESULTS: The liner ranges of atenolol, hydrochlorothiazide and nifedipine were 10～ 250? g? mL- 1 ( r=0. 999 4) , 6～ 150? g? mL- 1( r=0. 999 6) , and 5～ 125? g? mL- 1( r=0. 999 2) respectively. The average recovery rates of the three constituents were ( 98. 51? 0. 98) % ～ ( 99. 12? 0. 98) % , ( 98. 87? 1. 10) % ～ ( 99. 69? 1. 43) % , and( 98. 80? 1. 27) % ～ ( 99. 38? 1. 13) % , respectively, with RSD at 1. 00% , 1. 17% , and 1. 25% , respectively. CONCLUSION: The established method is simple, sensitive and reproducible, and suitable for the quality control of xueyaan sustained release tablets.
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OBJECTIVE: To prepare thermosensitive meloxicam hydrogel and establish its quality control method.METHODS: The hydrogel was prepared with poloxamer 407 and poloxamer 188 as base.The content of meloxicam in the thermosensitive gel was determined by UV spectrophotometry.RESULTS: The thermosensitive meloxicam hydrogel was yellowish or flavovirens in color,with its identification and tests all in conformity with the related specification stated in Chinese Pharmacopeia(2005 edition).The linear response range of meloxican was 1.956～19.56 mg?L-1(r=0.999 7).The average recovery was 98.42%(RSD=1.53%).CONCLUSION: The preparative technique is simple,and the quality of the preparation is controllable.
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OBJECTIVE:To assess the clinical efficacy and safety of domestic sparfloxacin for acute bacterial infections.METHODS:Domestic literatures about sparfloxacin for acute bacterial infections were retrieved by computer and their quality was evaluated to extract data(1993～2009).RevMan 4.2.2 software was used for Meta-analysis.RESULTS:A total of 10 RCT were enrolled.The comparisons of 2 groups were homogeneous in terms of clinical cure rate,clinical response rate,bacterial clearance rate and safety.There was statistical significance in comparison of combined effect variable between 2 groups in respect of cure rate,response rate and bacterial clearance rate(P0.05).CONCLUSION:The currently available evidence shows that clinical efficacy of domestic sparfloxacin for acute bacterial infections is better and incidence of ADR was lower.
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OBJECTIVE:To prepare praziquantel aquogel suppositories and to establish its assay method.METHOD:The optimal formula of the base was optimized by orthogonal experiment and the content of principal agent-praziquantel was determined by UV spectrophotometry.RESULTS:The optimized formula of the base was as follows,poloxamer P407∶P188(15%∶20%),sodium alginate 0.6%and ethyl alcohol 15%,the praziquantel aquogel suppositories prepared in this formula could be jellified under the body temperature with optimal viscosity.Good linear relationship occurred when the detection concentration of praziquantel was within a range of 0.2～10mg/ml(r=0.9 999),the average recovery of which was(102.44?0.69)%.CONCLUSION:The design of the formula is reasonable;the preparation process is reliable and the method for assaying is simple,fast and accurate.
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OBJECTIVE:To select the optimal formulation of thermosensitive secnidazole hydrogels and establish its determination method. METHODS:Formulation was optimized by orthogonal design with the content of poloxamer P407/P188(A),ethanol (B)and sodium alginate(C)as factors and with secnidazole as index. The content of secnidazole in preparation was determined by UV spectrometry at detection wavelength of 277 nm. RESULTS:The optimal formulation was as follows:A of 15 g/15 g,B of 20 mL and C of 0.6 g. The linear range of secnidazole was 4.618～46.18 mg?L-(1r=0.999 8)with an average recovery of 98.27%. The RSD of intra-day and inter-day were both lower than 1.8% and 2.1% respectively. CONCLUSIONS:The formulation is reasonable and the process was reliable and controllable in quality.
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OBJECTIVE: To prepare thermo-sensitive ornidazole hydrogel and establish its quality control method.METHODS: Thermo-sensitive ornidazole hydrogel was prepared with ornidazole as chief constituent using poloxamer 407 and poloxamer 188 as base.The content of ornidazole in the hydrogel was determined by ultraviolet spectrophotometry.RESULTS: The preparation was white or yellowish semisolid gel,and its test results were up to the related standard specified in Chinese Pharmacopeia(2005 Edition).The linear range of ornidazole was 3.98～43.77 mg?L-1(r=0.999 8),and its mean recovery was 98.52%(RSD=1.1%).CONCLUSION: The preparation is simple and feasible in preparation process,and the quality of the preparation is stable and controllable.