ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of 20% fluor-hydroxyapatite (FHA) on proliferation and osteogenic differentiation of human MG63 osteosarcoma cells.</p><p><b>METHODS</b>FHA was prepared by chemical precipitation method, and its structure and surface features were tested by scanning microscope, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy. MG63 cells were cultured and divided into FHA, hydroxyapatite (HA) and control groups (n = 3). The proliferation of the cells was evaluated using methylthiazol tetrazolium (MTT) assay. ALP activity of the cells was assessed. Osteogenic differentiation was evaluated based on the reverse transcription PCR (RT-PCR) of differentiation-related genes, namely, collagen type I (Col I), alkaline phosphatase (ALP), osteocalcin (OCN) and core binding factor α1 (Cbfα1). The data were analyzed statistically by one-way analysis of variance using SPSS 13.0 software.</p><p><b>RESULTS</b>XRD test showed that the main crystalline phase of FHA was similar to that of HA. Absorptance value of cells exposed to FHA(1.87±0.06) measured by MTT was higher than that of the control(1.25±0.02) on the third day(P < 0.05), and there was no statistically significant difference between the cells exposed to FHA and HA(1.84±0.03) (P > 0.05). ALP activity of the cells exposed to FHA(4.62±0.09)was higher than that of the control (1.92 ± 0.05) (P < 0.05). RT-PCR tests showed that compared with the control, FHA up-regulated the expression of Col I, ALP and OCN mRNA, down-regulated the expression of Cbfα1 mRNA.</p><p><b>CONCLUSIONS</b>FHA enhances the proliferation and osteogenic differentiation-related gene expression, and has good biocompatibility.</p>
Subject(s)
Humans , Alkaline Phosphatase , Genetics , Metabolism , Analysis of Variance , Biocompatible Materials , Bone Neoplasms , Metabolism , Pathology , Cell Differentiation , Genetics , Cell Proliferation , Physiology , Collagen Type I , Genetics , Metabolism , Core Binding Factor Alpha 1 Subunit , Genetics , Durapatite , Chemistry , Pharmacology , Hydroxyapatites , Osteocalcin , Genetics , Metabolism , Osteogenesis , Osteosarcoma , Metabolism , Pathology , Spectroscopy, Fourier Transform Infrared , X-Ray DiffractionABSTRACT
This study was aimed to analyze the volatile oil of Mentha crispata Schrad. ex Willd. in order to provide evidence for its chemotype and guidance for its production application. The chemical analysis was detected by headspace GC-MS. The results showed that 64 chemical compounds were detected. It was concluded that the volatile oil of M. crispata Schrad. ex Willd. mainly contained eucalyptol (35.58%), limonene (16.92%) and pinene (15.33%). It was concluded that the analysis on composition characteristics and main compounds of M. crispata Schrad. ex Willd. can provide evidences in its production application and chemotype.
ABSTRACT
<p><b>OBJECTIVE</b>To study effects of different harvest times and processing methods on the quality of Gardeniae Fructus.</p><p><b>METHOD</b>The content of asminoidin and the similarity of the fingerprint of Gardeniae Fructus were determined and applied for assessment of the quality.</p><p><b>RESULT</b>Gardeniae Fructus harvested in October with yellow-green appearance was the better time point for harvest. Drying in the sun and boiling in the water is the better processing method for Gardeniae Fructus.</p><p><b>CONCLUSION</b>Different harvest times and processing methods can affect the quality of Gardeniae Fructus.</p>