Expression and Prognostic Value of Cuprotosis-related Genes in Liver Cancer / 肿瘤防治研究

Objective To explore the relationship of cuprotosis-related genes with survival rate and prognosis in patients with liver cancer. Methods By collecting clinical information and corresponding RNA-seq data of patients with liver cancer in the TCGA database, the differential expression levels of 10 cuprotosis-related genes in liver cancer and normal tissues was analyzed. Novel liver cancer subtypes were identified through consistent clustering, and differences in overall survival and clinicopathological factors were compared between the two subtypes. Univariate Cox regression analysis was used in screening cuprotosis genes associated with prognosis, and LASSO regression analysis was used in constructing a risk model. Results FDX1 was down-regulated, and the other nine genes were up-regulated in HCC tissues compared with normal tissues. Cluster analysis showed that the prognosis of Cluster1 was poor. Five prognostic genes (LIPT1, DLAT, MTF1, GLS, and CDKN2A) were screened out through univariate Cox regression analysis and LASSO regression analysis for risk model construction. The risk score of this prognostic model was identified as an independent prognostic factor compared with other clinical features. Conclusion Through bioinformatics analysis, a liver cancer prognosis model of five cuprotosis-related genes is constructed, which may be used as molecular markers for tumor diagnosis and are potential therapeutic targets.

Analysis on the clinical effect of Yangxue Zhitong pills combined with Shuangbaisan on the treatment of children with hip synovitis / 中国生化药物杂志

To observe the clinical effect of Yangxue Zhitong pills combined with Shuangbaisan on the treatment of children with hip synovitis in.Methods 60 children with hip synovitis from November 2013 to May 2015 were randomly divided into the observation group and the control group,30 cases in each group.The observation group were given conventional treatment and the control group was given Yangxuezhitong pills combined with Shuangbaisan.Hip VAS and blood flow index in the two groups were followed-up and compared.Results (5 days after treatment,hip VAS in the observation group(3.50±0.46),was lower than that of the control group(4.51±0.55)(P<0.05).②CRP,TNF-α,IL-6,IL-1 level in the observation group were(3.01±1.73mg/L,15.58±5.46pg/mlL,74.9±19.4pg/mL,22.57±4.01ng/L),which were lower than those in the control group(5.69±2.05mg/L,32.47±4.16pg/mL,97.6±24.2pg/mL,32.17±4.38ng/L)(P<0.05).③After treatment,total effective rate in the observation group(93.3％)was higher than that in the control group 66.7％(P<0.05).Conclusion The clinical therapeutic effect of Yangxue Zhitong pill combined with Shuangbaisan on the treatment of children with hip synovitis is exact,which is worthy of further clinical research and application.

The design of non-viral nanometer vectors for delivery of small interfering RNA / 国际药学研究杂志

RNA interference(RNAi)is a method that allows double-stranded RNA(dsRNA)to induce the target gene mRNA to degrade in the body and lead to different levels of gene silencing. Small interfering RNA(siRNA)is the effector molecules of RNAi, and needs the delivery vehicle into the cells to play a therapeutic role. Nanoscale carrier can adjust the rate of drug release ,increase permeability of biofilm,change distribution in the body,and improve bioavailability. In this manuscript,materials,structure and bio?logical characteristics of existing non-viral nanometer vectors delivery for siRNA are summarized.