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The copy numbers of exogenous gene in transgenic animals is always regarded as an important information of transgenic animals.Thus,simple and sensitive methods are required for the detection of the copy numbers of exogenous gene.Three kinds of transgenic Shanbei white cashmere goats,containing Tβ4-GFP,FGF5s-GFP and VEGF164-GFP,has been obtained by using PiggyBac(PB) transposon system.Fluorescence quantitative PCR was carried out to detect the copy numbers of copGFP.Using Gluc as reference gene,the double standard curves of exogenous gene and reference gene were mapped and the genomic DNA of transgenic goats were analysized by real-time fluorescence quantitative PCR.Moreover,the copGFP/Gluc ratio in the samples was calculated as the copy numbers of copGFP.In addition,Tβ4-GFP transgenic cashmere goats were selected to detect the integration sites by using the genomic walking kit.The results showed that the standard curve equation of copGFP was y=-3.230 6x+39.216 (R2 =0.998 8) and the standard curve equation of Gluc was y=-3.564 8x+38.440 (R2 =0.996 0).The copy numbers of exogenous gene in the transgenic cashmere goats were obtained and the numbers of integration sites in the selected Tβ4-GFP transgenic goats were consistent with the copy numbers of copGFP.As a conclusion,the high throughput,fast and sensitive real-time fluorescence quantitative PCR is an efficient and convenient method for the copy number of exogenous gene in transgenic cashmere goats.
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Wearable technology, widely applied in health and medical field, has become an important part of in-telligent healthcare and medicare. However, the data security and privacy protection of wearable health and medi-cal devices are greatly concerned and need to be solved. The characteristics of data security and privacy protection of wearable health and medical devices were thus analyzed and compared with those of traditional health and medi-cal devices. The related studies and laws on domestic and foreign wearable health and medical devices were summa-rized, and the advances, experiences and problems in domestic and foreign wearable health and medical devices were analyzed and compared in order to provide reference for establishing the mechanisms underlying their data se-curity and privacy protection.
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The index system of comparative advantages was constructed for the pharmaceutical manufacturing in-dustry in different provinces , municipalities directly under the Central Government , and autonomous regions ac-cording to the integrated comparative advantage theory .The integrated comparative advantages in different provinces , municipalities directly under the Central Government , and autonomous regions were scored by principal component analysis and qualitatively analyzed by clustering analysis to reveal their spatial distribution , thus providing the deci-sion-making support for the policy makers, manufacturers, investors and staff of pharmaceutical manufacturing in-dustry.
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The Web portals of health information management organization gateway in USA, Canada and Australia were analyzed in aspects of their leading organizations , Web resources , types of user , relationship between users and resources in order to know their methods to organize resources and Website design , and certain suggestions were put forward for the Web portal construction of official gateway and related health information service institutions .
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Hepatits B virus( HBV) infection is a global epidemic which seriously harms the public health. In spite of the great progress in hepatits B prevention and treatment, there is few ef-fective medicine. Research findings show that liver damage and degree of liver failure are sophisticatedly related to the interaction between HBV and the immune response of host. All these make it important to know the replication mechanism and the contrac-tion process, in order to lay a preliminary solid foundation for studying HBV drug targets and making a new ant-virus strategy. This article aims to summarize HBV viral replication process, while focusing on the latest research findings about drug targets, to find a new kind of anti-HBV drugs, and to explore the under-lying mechanism of effective drugs.
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Objective To investigate the clinical significance of platelet activation markers CD62p and PAC-1 in patients with hypertension and coronary heart diease.Methods To select patients who were in the Second Affiliated Hospital of Dalian Medical University between January 2012 and September 2012,42 patients with hypertension,46 patients with coronary heart disease.Flow cytometry was used to test the positive percentage of the platelet surface activation markers CD62p and PAC-1 .To observe the difference among hypertension group,coronary heart disease group and health control group;hyper-tension and coronary heart disease group.Results The positive percentage of platelet activation markers CD62p and PAC-1 in hypertension (36.36±9.62,7.18±8.20)%,coronary heart disease group (42.74±14.60,8.81±12.53)% showed sta-tistically significant differences (t=4.150~5.853,P<0.01)compared with healthy control group (26.82±9.13 ,1.09± 1.05)%.The positive percentage of CD62p in coronary heart disease group (42.74±14.60)% was also higher than that of hypertension (36.36±9.62)%,and the difference between them was of statistical significance (t=2.444,P<0.05).Con-clusion The hypertensive patients and coronary heart disease were in pre-thrombotic state,the activation of platelet increase significantly.Molecular markers should be measured in hypertensive patients and coronary heart disease for prevention and treatment of thrombotic disease complication.
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OBJECTIVE@#To compare the effect of 7 antipsychotic drugs on the life quality of schizophrenia patients including chlorpromazine, sulpiride, clozapine, risperidone, olanzapine, quetiapine, and aripiprazole.@*METHODS@#A total of 1,227 stable schizophrenic patients within 5 years onset who took 1 of the 7 study medications as maintenance treatment were followed up for 1 year at 10 China sites. Patients were evaluated by the short form-36 health survey (SF-36) at the baseline and at the end of 1 year.@*RESULTS@#The life quality was improved obviously at the end of the follow-up. There was significant difference in body pain, vitality, and mental health (P<0.05) among these antipsychotic drugs.@*CONCLUSION@#All 7 antipsychotic drugs can improve the life quality of schizophrenia patients. Atypical antipsychotic drugs, especially olazapine and quetiapine, are superior to typical antipsychotic drugs in improving life quality.
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Antipsychotic Agents , Therapeutic Uses , Benzodiazepines , Therapeutic Uses , Dibenzothiazepines , Therapeutic Uses , Follow-Up Studies , Olanzapine , Quality of Life , Quetiapine Fumarate , Schizophrenia , Drug Therapy , Surveys and QuestionnairesABSTRACT
Non-proline cis peptide bond is rarely found in proteins. The recent surveys revealed that this unusual peptide configuration is by no means a curiosity, but overwhelmingly occurs at functionally important sites. However one still doubts whether it is related to crystal packing interactions, since all non-proline cis peptide bonds identified so far are from crystal structures. A toxin BmK M1 from the scorpion Buthus martensii Karsch have been crystallized as a dimer in space group P212121 with unit-cell dimensions a = 76.39 (A),b=52.77 (A), c=27.12 (A). This dimeric structure was solved by molecular replacement and refined to R=0.109 for all reflections at a resolution of 1.4 (A). The extensively refined structure definitely shows that the cis peptide bond Pro9-His10 equally occurs in both molecule A and molecule B in the dimer. The observation manifested that this striking non-proline cis peptide is not related to crystal packing, but caused by certain intrinsic factors. The detailed analyses and comparison with the structure of BmK M8, which is homologous to M1 but has trans peptide bond 9-10, showed that the five-residue reverse (8 ～12) with a consensus sequence (-KPXNC-) may be the intrinsic structural element for the cis form of this peptide bond. A pair of well organized main-chain hydrogen bond between residues 10 in cis unit and 64 at C-terminus forms main tertiary interactions to stabilize this energetically unfavorable peptide bond.
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BACKGROUND: The study on the candidate gene of schizophrenia was one of the major ways of exploring its etiology. One of important candidate genes associated with schizophrenia is the brain-derived neurotrophic factor gene(BDNF)OBJECTIVE: To explore the association between schizophrenia and C270T polymorphism of BDNF gene.DESIGN: Case-control and comparative observation SETTING: Institute of Mental Health of Central South University PARTICIPANTS: Totally 194 patients with schizophrenia, including 95 males and 99 females aged from 15 to 59 years, hospitalized in man and women wards in the Institute of Mental Health, Xiangya Second Hospital of Central South University from March to October 2003 were set as patients group. Controls were selected among the healthy volunteers of Xiangya Medical College was included in terms of age and gender comparable with patients group. Altogether 187 cases of controls, of whom 88 males and 99 females were studied. Their age ranged from 18 to 42 years old with the average of (26±7) years old.Those with psychosies and severe somatopathy were excluded. The patients and their family member have no history of psychosis .All the subjects were of Hunan Han nationality. Either the patient or his or her family members signed the written consent form. Individuals in the control group also signed the written consent form.positive symptom scale (PANSS-P) (7 items), negative symptom scale (PANSS-N) (7 items) and General Symptom Scale (PANSS-G) (16 items),altogether 30 items, as well as 3 additional items to evaluate the attack fatalness were used to evaluate whether the psychic symptom existed or not and the degree of severity of each symptom(7-item scoring: 1 was without,7 was very severe) . The patients were evaluated on the day of hospitalizing was used to compare the frequency among groups.polymorphism of BDNF gene between patients group and healthy control RESULTS: Totally 194 patients with schizophrenia and 187 healthy genotype (26.8%) in patients was much higher than that of the healthy controls (5.9%) (x2=32.71, df=1, P ＜ 0.01).The distribution frequency of T allele in patients' group (14.4%) was much higher than that in the scores, PANSS-P, PANSS-N and PANSS-G scores in patients with the genotype of C/T were 59-121 ;9-42( average 20.08±6.16);8-41 (average 19.02±9.13);22-68 (average 36±8.02)respectively. And the total PANSS scores, PANSS-P, PANSS-N and PANSS-G scores in patients with the genotype of C/C were 58-121 ;7-39, (average 19.2±5.88);8-40scores, (average19.02 ±8.98); 22 -68 (average36.4 ±8.32)respectively.There were no significant differences in positive and negative symptom scale (PANSS)items as well as PANSS-G between the patients with C/C and C/T genotype(P ＞ 0.05).CONCLUSION: BDNF gene C270T polymorphism was associated with the susceptibility of schizophrenia. The distribution frequency of T/C genotype and T allele in schizophreniac was significantly higher than those of the normal healthy controls. And no significant difference was obtained between patients with the genotype of T/C or C/C in terms of negative,positive symptoms and psychosocial function as well.
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Objective: Our aim was to study whether the cellular bronchoalveolar lavage fluid (BALF) profile was associated with the parameters of pulmonary function tests. Methods：Lung function tests and BAL were carried out in 18 untreated, non-smoking patients suffering from sarcoidosis and 18 normal subjects. Results：（1）Lung function tests were normal at rest in patients with sarcoidosis（P＞0.05）, the single breath carbon monoxide diffusing capacity decreased (P＜0.05)，and the small airway function decreased too (P＜0.05).（2）The percentage of lymphocytes in BALF of sarcoidosis increased comparing with the normal subjects（P＜0.01），and the ratio of CD+4 to CD+8 in BALF increased significantly in sarcoidosis（P ＜0.05）, furthermore, the increase of both the percentage of lymphocytes and the ratio of CD+4 to CD+8 in BALF of sarcoidosis were well nagatively correlated with the decrease of the percentage of DLCO (r=-0.67, P＜0.01 and r=-0.55, P＜0.05, respectively), the decrease of mid-expiratory flow at 50% of vital capacity (FEF50%) was well correlated with the increase of the percentage of lymphocytes in BALF of sarcoidosis (r=-0.54, P＜0.05). Conclusion：Pulmonary diffusing capacity（DLCO) was significently correlated with BALF lymphocyte phenotype，so was small airway function，and can act as the marker of activity of sarcoidosis.
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AIM: To investigate the antagonistic action of Captopril (Cap) on the activation and injury of human umbilical vascular endothelial cells (HUVECs) induced by lipopolysaccharide(LPS) and the possible mechanisms. METHODS: After 18 h exposure of the cultured HUVECs to LPS (1 mg/L), or LPS (1 mg/L) plus Cap at the concentration of 10~(-7)mol/L, 10~(-5)mol/L and 10~(-3)mol/L, the expression of vWF protein in the conditioned media was tested by enzyme-linked immunosorbent assay (ELISA), the expression of ICAM-1 protein in HUVECs was determined by indirect immunofluorescence technique with flow cytometry as well. In addition, the expression of TNF? mRNA was determined by in situ hybridization. RESULTS: The results of ELISA and indirect immunofluorescence technique showed that exposure to LPS at a concentration of 1 mg/L led to a significant increase in the vWF and ICAM-1 expression in HUVECs as compared to the control (P