ABSTRACT
Objective:To evaluate the effects and underlying molecular mechanism of salvianolic acid B(Sal B) on autophagy in glioma cell.Methods:The C6 cells were treated with Sal B at a concentration ranging from 0 to 400 μmol/L and the cell viability were measured by CCK8 assay.Flow cytometry was performed for apoptosis,Western blot assay for protein expression and the expression of LC3 was calculated by immunofluorescence.Results:The cell viability was weakened by Sal B at a concentration of 100 μmol/L, thus,the concentration of 10,20 and 50 μmol/L were selected for further experiment.Sal B dose-dependedly induced C6 cell apoptosis and the expression of caspase-3 and Bax,notably decreased the protein level of Bcl-2 and Ki67.Meanwhile,Sal B significantly up-regulated the expression level of Atg5,Atg7,Atg12 and Beclin1,increased the ratio of LC3Ⅱ/Ⅰand the amount of LC3-labeled puncta per cell,and down-regulated the expression of p62.In addition,the protein level of mTOR was makedly decreased by Sal B and Sal B induced the expression of Ulk1.Conclusion:Sal B induces autophagic cell death in glioma cell through mTOR/Ulk1 signaling pathway.
ABSTRACT
@#ObjectiveTo observe the effects of acupuncture combined with massage on shoulder subluxation and motor function recovery of the upper extremities in patients with hemiplegia after stroke.Methods60 hemiplegic patients were randomly divided into two groups for treatment with acupuncture cooperates with massage or usual rehabilition, respectively. The recovery of the patient's shoulder subluxation and movement function of upper extremities were evaluated 2 months after treatment. ResultsThe shoulder subluxation and movement function of the upper extremities were improved after treatment with both therapies (P<0.01), and acupuncture cooperates with massage showed better effect (P<0.01). ConclusionAcupuncture cooperates with massage can facilitate the recovery of shoulder subluxation and motor function of the upper extremities after hemiplegia.