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Micronucleus ( MN) assay as a routine examination for genotoxicity has been widely used.The testing specimens were taken from bone marrow and extended from blood and tissues.In addition to testing genotoxicity of drugs, it is also applied in disease diagnosis for genetic mutation, evaluation of curative effectiveness and disease prevention. Moreover, MN assay is also an important safety indicator for drugs and health foods registration.This review will discuss the staining method of MN test and its application in the field of diseases and virology.
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Objective To train medical students' competence of independent thinking and problem solving and to improve the quality of teaching.MethodsThe case-based PBL teaching model was introduced to teaching practice for 25 undergraduates majoring in medical laboratory.The teaching process included pre-class mobilization,question,self-study,discussion and summary.Effectiveness of teaching was evaluated with questionnaire.Results For these medical students,the enthusiasm of learning ( 83.3％ ),ability of self-learning and information seeking ( 79.2％ ),ability of discussing and problem solving ( 79.2％ ),ability of scientific thinking and clinical reasoning ( 79.2％ ) were significantly improved.Teaching satisfaction rate of case-based PBL teaching model was up to 95.8％.ConclusionBetter results of teaching were achieved and the case-based PBL teaching model is worth spreading.
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Objective We searched for optimal methods of embolization according to the images of selective hepatic arteriography to improve the efficiency of intervention.Methods 40 patients were selected to embolic with lipiodol ultra-fluida and PYM and followed by 6～48 months.Results According its shape and sign of stainangtime,liver hemangioma was distincted from liver cancer.The hemangioma sizes of 30 cases reduced beyond 50 percent and 4 cases had no change.All diminished hemongioma signs can been detected by ultrasound.The effective rate reached 85 percent.All patients had no side-effect.Conclusion The treatment of hepatic hemangioma with selectic arteriography and embolization is a good way in diagnosis and handle.
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AIM: To study ba cteriostasis activities of ligand (2-(carboxymethoxyl) benzaldehyde benzoyl hyd razone (H 2L)) and their complexes. METHODS: Ligand H 2L and t heir complexes were synthesized. The bacteriostasis activities of H 2L and thei r complexes in vitro were tested with K-B method. RESUL TS: 2-(carboxymethoxyl) benzaldehyde benzoyl hydrazone only showed bact eriostasis activities on Staphyloccus aureus and their complexes had dramatic bacteriostasis activity on Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. Bacteri ostasis ring diameter of Cu-complex was largest. CONCLUSION: L igand H 2L complexes have bacteriostasis activities in vitro. B acteriostasis activities of Cu-complex are strongest among the complexes.
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AIM: To study inhibitive effect of Lagotis Brevituba Maxim extracted on human stomach cancer SGC-7901 cells proliferation and/or induce apoptosis. METHODS: Human stomach cancer SGC-7901 cells were treated with Lagotis Brevituba Maxim extracted from n-butanol of 0.34～21.60 mg/mL for 24 h～72 h.Cell proliferation was evaluated by MTT assay.Morphological changes of apoptosis were examined by fluorescence microscope and electron microscope.DNA fragmentation was visualized by agarose gel electrophoresis.The amount of apoptosis cells was measured by flow cytometry. RESULTS: Growth of SGC-7901 cells was obviously inhibited by Lagotis Brevituba Maxim extract with value from 21.60 mg/mL to 0.34 mg/mL.After incubation of SGC-7901 cells with 2.7 mg/mL Lagotis Brevituba Maxim extract for 48 h, morphological changes of apoptosis were observed.DNA ladder was identified by agarose gel electrophoresis of DNA ladder. CONCLUSION: The n-butanol extract of Lagotis Brevituba Maxim can inhibit SGC-7901 cell growth and induce apoptosis.