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Liver regeneration following injury aids the restoration of liver mass and the recovery of liver function. In the present study we investigated the contribution of megakaryocytic leukemia 1 (MKL1), a transcriptional modulator, to liver regeneration. We report that both MKL1 expression and its nuclear translocation correlated with hepatocyte proliferation in cell and animal models of liver regeneration and in liver failure patients. Mice with MKL1 deletion exhibited defective regenerative response in the liver. Transcriptomic analysis revealed that MKL1 interacted with E2F1 to program pro-regenerative transcription. MAPKAPK2 mediated phosphorylation primed MKL1 for its interaction with E2F1. Of interest, phospholipase d2 promoted MKL1 nuclear accumulation and liver regeneration by catalyzing production of phosphatidic acid (PA). PA administration stimulated hepatocyte proliferation and enhanced survival in a MKL1-dependent manner in a pre-clinical model of liver failure. Finally, PA levels was detected to be positively correlated with expression of pro-regenerative genes and inversely correlated with liver injury in liver failure patients. In conclusion, our data reveal a novel mechanism whereby MKL1 contributes to liver regeneration. Screening for small-molecule compounds boosting MKL1 activity may be considered as a reasonable approach to treat acute liver failure.
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Objective:To evaluate the current organizational capacity and maturity of private hospitals in China, in references improving their organizational capacity and promoting their sustainable development.Methods:The purposive sampling method was used from May to November 2019, and private hospitals of continuous operation for 5 years or above were selected for a questionnaire survey. Self-evaluation was made on the organization capacity of these hospitals in such 12 aspects as positioning, normalization, decision making, execution, supervision, marketing, coerciveness, risk exposure, innovation, learning capacity and leadership. Based on existing models, five levels of maturity models from low to high were established, namely that of business in difficulty, that of basic stable operation, that of stable development, that of expanding business, and that of independent branding.Factor analysis was used for structural efficiency analysis, the questionnaires were subject to a descriptive analysis, and a maturity scoring was obtained based on self-evaluation of organizational capacity.Results:A total of 45 non-public hospitals were investigated in this study, and 450 valid questionnaires were recovered. The factor analysis classified the organizational capacity into basic capacity, core capacity and development capacity. The organizational capability of the hospitals scored in average 4.14 points; the development capacity scoring(3.87 points) was lower than basic capacity(4.16 points) and core capacity(4.06 points). 80% of the private hospitals were in the basic stable stage or stable development stage.Conclusions:Private hospitals feature in general lower maturity, good basic capacity and core capacity, while they tend to have weak development capacity, not to mention innovation and construction of learning organizations.
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Objective:To investigate and compare the attitudes of clinical nurses and nursing managers towards float nurse, and to provide references for the implementation of nurse mobile practice mode in China.Methods:By stratified sampling, the survey was conducted on clinical nurses and nursing managers of 15 tertiary hospitals in 9 prefecture-level cities in Fujian.Results:A total of 9 389 valid questionnaires were recovered, including 525 for nursing managers and 8 864 for clinical nurses. By comparing the attitudes between two groups, it was found that nursing managers who knew more about mobile practice were more willing to practice floating practice, including 50.86% (267/525) heard and inquired, 49.14% (258/525) willing to float, than clinical nurses who paid less attention, including 32.41% (2 873/8 864) heard and inquired, 38.87% (3 445/8 864) willing to float. Multi-factor analysis showed that among nursing managers, those with a bachelor’s degree or above was more willing to float. While among clinical nurses, young, unmarried, side, bachelor degree or above were more willing to float. On the choice of flow mode, nursing managers were inclined to float between hospitals (46.54%, 215/525), but clinical nurses were prone to community float (42.45%, 3 142/8 864). Furthermore, nursing managers had higher requirements on the professional title and seniority of float nurse, and 39.43% (207/525) believe that the professional title should be supervisor, while 81.71% (429/525) thought that length of service as a nurse should be 5 years or more.Conclusion:There are differences between clinical nurses and nursing managers in such aspects as the intention of floating practice, obstacles and guarantee factors, cognition and selection of demanders, and acceptance of practice threshold. The above differences should be fully considered in the trial of the "Internet + Nursing" floating practice mode for nurses.
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Objective@#To investigate and compare the attitudes of clinical nurses and nursing managers towards float nurse, and to provide references for the implementation of nurse mobile practice mode in China.@*Methods@#By stratified sampling, the survey was conducted on clinical nurses and nursing managers of 15 tertiary hospitals in 9 prefecture-level cities in Fujian.@*Results@#A total of 9 389 valid questionnaires were recovered, including 525 for nursing managers and 8 864 for clinical nurses. By comparing the attitudes between two groups, it was found that nursing managers who knew more about mobile practice were more willing to practice floating practice, including 50.86% (267/525) heard and inquired, 49.14% (258/525) willing to float, than clinical nurses who paid less attention, including 32.41% (2 873/8 864) heard and inquired, 38.87% (3 445/8 864) willing to float. Multi-factor analysis showed that among nursing managers, those with a bachelor’s degree or above was more willing to float. While among clinical nurses, young, unmarried, side, bachelor degree or above were more willing to float. On the choice of flow mode, nursing managers were inclined to float between hospitals (46.54%, 215/525), but clinical nurses were prone to community float (42.45%, 3 142/8 864). Furthermore, nursing managers had higher requirements on the professional title and seniority of float nurse, and 39.43% (207/525) believe that the professional title should be supervisor, while 81.71% (429/525) thought that length of service as a nurse should be 5 years or more.@*Conclusion@#There are differences between clinical nurses and nursing managers in such aspects as the intention of floating practice, obstacles and guarantee factors, cognition and selection of demanders, and acceptance of practice threshold. The above differences should be fully considered in the trial of the "Internet + Nursing" floating practice mode for nurses.
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Objective: To explore the mechanism of Yinao Jieyu Compound Formula in the treatment of post-stroke stressrats from the perspective of intestinal flora changes. Methods: A total of 48 male clean SD rats were randomly divided into4 groups: sham-operation group, post-stroke stress group, western medicine group and traditional Chinese medicinegroup, with 12 rats in each group. In the sham-operation group, only the free blood vessels were not inserted into thethread embolus. The rats in post-stroke stress group, the western medicine group and the Chinese medicine group weretreated as follows: after 1 week of middle cerebral artery occlusion, the rat model of PSD was prepared by using chronicrestraint stress and single cage feeding. The 7 th day from the beginning of the construction of the cerebral stroke model, the gavage treatment began to be done. The western medicine group was given Fluoxetine Hydrochloride Capsules2.33 mg/ (kg.d), and the Chinese medicine group was given 9.92 g/ (kg·D) . The rest of the rats were gavage with 10 mL/kgdistilled water, 1 time/day. The behavioral changes on the 14 th day after post-stroke were observed and the microbialcommunity structure and composition was analyzed by Illumina-MiSeq high throughput sequencing technique. Results:Compared with the sham operation group, the sucrose water preference, open field score and swimming struggle time ofthe post-stroke stress group were significantly decreased (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01), and the proportions ofAllobaculum, Sutterella, Dorea and rc4-4 in the feces decreased significantly (P ＜ 0.01, P ＜ 0.01, P ＜ 0.01, P ＜ 0.05) .The proportion of Oscillospira increased significantly (P ＜ 0.01, P ＜ 0.05, P ＜ 0.01) . Compared with the post-strokestress group, the horizontal scores and swimming struggling time of the rats in the Chinese medicine group weresignificantly increased (P ＜ 0.05, P ＜ 0.05), and the proportions of the 6 species of Lactobacillus and Bacteroides weresignificantly increased in the feces. The difference between Lactobacillus and Bacteroides was the most obvious with27.91％ vs 5.96％ and 24.21％ vs 9.62％ (P ＜ 0.01, P ＜ 0.01) . The proportion of Oscillospira was significantly decreased (P ＜ 0.05) . Compared with the western medicine group, there was no significant difference in the behavioral scores of therats in the traditional Chinese medicine group (P＞ 0.05), and the proportion of Bacteroides and rc4-4 in the feces wassignificantly increased (P ＜ 0.01, P ＜ 0.05) . Conclusion: Yinao Jieyu Compound Formula can alleviate similar depressivesymptoms in post-stroke stress rats by increasing the content of Lactobacillus and Bacteroides in the intestine of rats.
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Objective To investigate the intervention effects ofYinao Jieyu Prescription on the behaviors and damages in hippocampal CA1 area of the rats with post-stroke depression (PSD).Methods Totally 168 SPF male SD rats were randomly divided into normal group, sham-operation group, stroke group, PSD group, Western medicine group and TCM group. There were 24 rats in the normal group and sham-operation group, and 30 rats in the other groups. Rats in the normal group received no intervention. Rats in the sham-operation group received no suture. Rats in the stroke group were given middle cerebral artery occlusion operation and normally fed after operation. Rats in the PSD group, Western medicinal group and TCM group were made into PSD models by chronic immobilization stress for one week and individual battery to the end. At the inception of modeling, Western medicine group received fluoxetine hydrochloride for gavage; TCM group receivedYinao Jieyu Prescription for gavage; other groups received distilled water for gavage, once a day. At the end of week 2, 4, and 8, the morphology of the hippocampal CA1 area in each rat was observed by microscope after HE stained.Results Except for the week 2, at the same time point, the behavior scores of the rats in the TCM group were higher than those in the PSD group. At the same time point, the CA1 region of the hippocampus in the TCM group was more complete than the PSD group, and the cells were arranged neatly and in normal morphology.ConclusionYinao JieyuPrescription can improve the symptoms of PSD rats, and has protective effects on hippocampal CA1 area.