ABSTRACT
<p><b>OBJECTIVE</b>To study the etiological role of human papillomavirus type 16 (HPV16) infection in the development of esophageal cancers.</p><p><b>METHODS</b>A recombinant retrovirus containing the E6E7 ORFs of HPV16 was packaged and human fetal esophageal fibroblasts were infected. The tumorigenecity of the fibroblasts was tested in SCID mice in synergy with 12-O-tetradecanoylphorbol-13-acetate (TPA).</p><p><b>RESULTS</b>Human esophageal fibroblasts infected with the recombinant retrovirus induced sarcomas in SCID mice, the existence and expression of E6E7 ORFs was confirmed in the sarcomas. Fibroblasts cultured from the sarcoma were demonstrated heteroploid by cytoflowmetry. However, tumors were not observed in human fetal esophagus infected with such virus.</p><p><b>CONCLUSIONS</b>These results revealed that the established recombinant retroviral system can successfully mediate the transference of HPV16 E6E7 genes, and such system is applicable to researches on tumorigenesis of HPV.</p>
Subject(s)
Animals , Humans , Mice , Cell Transformation, Neoplastic , Cells, Cultured , Esophagus , Pathology , Virology , Fetus , Fibroblasts , Metabolism , Virology , Human papillomavirus 16 , Genetics , Mice, SCID , Oncogene Proteins, Viral , Genetics , Metabolism , Open Reading Frames , Genetics , Papillomavirus E7 Proteins , Recombination, Genetic , Repressor Proteins , Genetics , Metabolism , Retroviridae , Genetics , TransfectionABSTRACT
<p><b>BACKGROUND</b>To study the deletion and mutation in carboxy terminal region of LMP1 gene derived from nasopharyngeal carcinoma (NPC) in Guangdong and Guangxi, the high risk areas of nasopharyngeal carcinoma in China.</p><p><b>METHODS</b>LMP1 gene carboxy terminal region was amplified from nasopharyngeal carcinoma tissues by PCR, and then cloned and sequenced.</p><p><b>RESULTS</b>Of the 20 cases, 17 were LMP1 positive. In all positive cases, only 1 case did not show deletion. Four positive cases were chosen for DNA sequencing, The rusult showed that all the four cases had mutation and the 30bp deletion.</p><p><b>CONCLUSIONS</b>High frequency of deletion and mutation in LMP1 gene of nasopharyngeal carcinoma tissues was found in Guangdong and Guangxi. Whether it related to the high incidence of NPC should be further studied.</p>
Subject(s)
Humans , Amino Acid Sequence , Base Sequence , Gene Deletion , Herpesvirus 4, Human , Genetics , Molecular Sequence Data , Nasopharyngeal Neoplasms , Virology , Point Mutation , Sequence Analysis, DNA , Viral Matrix Proteins , GeneticsABSTRACT
<p><b>BACKGROUND</b>To study the relationship of Epstein-Barr virus (EBV) and T cell lymphoma.</p><p><b>METHODS</b>Sixty cases of T cell lymphomas were examined for the presence of EBV using in situ hybridization for EBV encoded RNA (EBERs).</p><p><b>RESULTS</b>EBERs were detected in tumor cells in 37(69.8%) of 53 cases with peripheral T cell lymphoma, but in none of seven cases of precursor T lymphoblastic lymphoma. The total detected EBERs were 37(61.6%) in 60 cases of T cell lymphomas. By Revised European-American Lymphoma(REAL) classification, EBERs were detected in 2/2 angioimmuno-blastic T cell lymphoma,17/18 angiocentric lymphoma, 4/6 anaplastic large cell lymphoma and 14/27 peripheral T cell lymphoma, unspecified (51.9%). The frequency of EBERs among the extranodal peripheral T cell lymphoma was higher than the nodal (P less than 0.01) there was no significant correlation with the sex, age and clinical stage.</p><p><b>CONCLUSIONS</b>This study indicated that high incidence of EBV was observed in peripheral T cell lymphoma, with predilection for angiocentric lymphoma and extranodal presentation.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , DNA, Viral , Genetics , Epstein-Barr Virus Infections , Pathology , Virology , Herpesvirus 4, Human , Genetics , Lymphoma, T-Cell , Pathology , Virology , Tumor Cells, CulturedABSTRACT
<p><b>OBJECTIVE</b>To identify and assess multiple human papillomavirus types in condyloma acuminatum lesions from patients with genital warts in Beijing area, and compare different features between otherwise healthy and immunosuppressed patients.</p><p><b>METHODS</b>PCR, RFLP and nucleotide sequencing analysis were used to determine HPV types from individual lesions.</p><p><b>RESULTS</b>The predominant type from other healthy patients was HPV6, secondly HPV11. The mean age of patients infected by HPV6 was lower than that of HPV11 and HPV6 + 11. While lesions from immunosuppressed patients were often contained HPV11 or mixed with HPV6. Besides, HPV types 16 and 53 were detected from infected lesions than other HPV types.</p><p><b>CONCLUSIONS</b>HPV6 was the major pathogen of condyloma acuminatum, but infected patients were at lower ages. While HPV11 was most often detected from immunosuppressed patients. As a low risk virus in normal genital tract, HPV53 also could be a pathogen in genital warts.</p>