SSTR2 mRNA expression and correlation with proliferating cell nuclear antigen in human colorectal cancer / 第三军医大学学报

Objective To investigate somatostatin receptor subtype 2 (SSTR2) mRNA expression and its correlation with proliferating cell nuclear antigen (PCNA) in human colorectal cancer. Methods A total of 60 colorectal cancer samples were analyzed. The SSTR2 mRNA expression was examined by in situ hybridization (ISH) using multiphase oligoprobes. The PCNA were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative contents of SSTR2 mRNA. Results The positive rate of expression of SSTR2 mRNA and relative contents in early and well-differentiated colorectal cancer were higher than those in advanced and poorly differentiated lesion. A negative correlation between SSTR2 mRNA expression and PCNA was found in colorectal cancer. Conclusion In the samples of colorectal cancer, the expression of SSTR2 mRNA is correlated with the degree of differentiation and clinical stage, and it plays an important role in reflecting the degree of malignancy and prognosis.

Relationship between expression of somatostatin receptors subtype 2 mRNA and estrogen and progesterone receptors in breast cancer / 中华医学杂志(英文版)

<p><b>OBJECTIVES</b>To observe the expression of somatostatin receptor subtype 2 (SSTR2) mRNA, and investigate the relationship between the expression of SSTR2 mRNA and the expressions of estrogen and progesterone receptors (ERs and PRs) in benign and malignant breast tissues.</p><p><b>METHODS</b>Samples from a total of 23 breast carcinomas, 16 mammary hyperplasias, and 9 mammary fibroadenomas were analyzed. SSTR2 mRNA expression was examined by in situ hybridization using multiphase oligoprobes. ER and PR expressions were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative content of SSTR2 mRNA.</p><p><b>RESULTS</b>The rate of expression (87.0%) and relative content (0.47) of SSTR2 mRNA in breast cancer were higher than those in benign breast tissue (64%, 0.26) (P < 0.05). SSTR2 mRNA expression was closely correlated with ER and PR expressions in breast cancer (P < 0.05). SSTR2 mRNA was also positively correlated with ER expression in benign breast tissues.</p><p><b>CONCLUSIONS</b>SSTR2 mRNA expression is higher or in benign breast tissues than in malignant ones. There is a significant positive correlation between SSTR2 mRNA and ER and PR expressions. Combined antiestrogen and somatostatin analogue in treatment of ER-positive breast cancers should be further investigated.</p>

An experimental study on the effect of somatostatin in antagonizing the maladjustment of sexual hormones homeostasis induced by tamoxifen / 重庆医科大学学报

Objective:To investigate the effect of somatostatin (SST) analog octreotide (OCT) in antagonizing the maladjustment of sexual hormones homeostasis induced by tamoxifen(TAM) in rat.Methods:Eight weeks after DMBA(a single dose 100mg/kg body weight by subcutaneous injection) application,the female Wistar rats were randomized to control and treatment groups.Rats in treatment groups underwent TAM(1mg/kg 5 times weekly for 14 weeks by subcutaneous injection),OCT(0.1mg/kg bid for 14 weeks by subcutaneous injection),or the combination of both.The levels of sexual hormones (estradiol,progesterone,prolactin,follicle stimulating hormone,luteinizing hormone) were assessed by enzyme immunoassay(EIA).Results:The levels of estradiol and progesterone in serum were remarkably higher in TAM group(21.69pg/ml,6.14ng/ml) than in control group(16.92pg/ml,4.13ng/ml) respectively(P