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Objective:To establish the Sunitinib-resistant model GIST882-Rs.Methods:The intermittent concentration gradient doubling method was used to get Sunitinib-resistant cell line GIST 882-Rs. Changes of cell morphology were observed and compared under the opticalm icroscopy. Changes of cell cycle were assayed and compared by using flow cytometer. The growth curvature , half maximal inhibitory concentration (IC50) were calculated by using cell counting kit (CCK8) assay.Results:Significant changes occurred in the morphologic profiles, such as epithelioid shape, the increased ratio of nucleolus to cytoplasm, emerging multinucleated cel1 and increase of granule sub stances. IC50 for GIST882 and GIST 882-Rs were(127.50 ± 2.15) μmol and (23.98 ± 1.85) μmol, and there were significant differences ( P < 0.05). The resistance index of GIST882-Rs to Sunitinib was 5.30 fold of GIST882 cells. The cell cycle of GIST882 and GIST882- Rs was respectively 52.51% and 39.87% in S phase 25.22% and 32.32% in G 0/G 1 phase. Conclusions:The sensitivity of GIST 882-Rs to Sunitinib treatment is decreased significantly.
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Objective To investigate the correlation between serum interleukin 1β (IL-1β) ,C reactive pro-tein (CRP) and homocysteine (Hcy) levels in elderly patients with ischemic stroke after fatigue and ischemic stroke .Methods 119 elderly patients with ischemic stroke were selected from our hospital from August 2016 to August 2017 .According to the fatigue severity scale ,40 cases were divided into fatigue group and 79 cases in non fatigue group .The peripheral blood samples were taken from the fasting blood in early morning ,and the serum levels of IL-1β ,CPR and Hcy were determined by enzyme linked immunosorbent assay (ELISA ) . Results The fatigue group ADL score ,Fugl-Meyer motor function of upper and lower limbs were lower than those of non fatigue group and control group ,the difference was statistically significant (non fatigue group :t＝23 .360 9 ,18 .203 6 ,9 .288 6 ,the control group :t＝39 .926 6 ,20 .260 3 ,21 .929 6 ,P＜0 .05) ;Non fatigue group ADL score ,Fugl-Meyer motor function of upper and lower limbs score lower than the control group ,the difference was statistically significant (t＝23 .021 4 ,6 .831 9 16 .810 3 ,P＜0 .05) .Fatigue serum IL-1β ,CPR and Hcy were higher than that in non fatigue group and control group ,the difference was statistically signifi-cant(non fatigue group :t＝14 .561 5 ,17 .029 6 ,13 .059 9 ,the control group :t＝31 .631 9 ,36 .998 3 ,33 .670 0 , P＜0 .05) ;Non fatigue group serum IL-1β ,CPR and Hcy were higher than that of control group ,the difference was statistically significant (t＝23 .866 0 ,28 .774 4 ,26 .135 4 ,P＜0 .05) .There was a positive linear correla- tion between fatigue and IL-1 beta ,CPR and Hcy in patients with ischemic stroke .Conclusion IL-1 ,CPR and Hcy levels in elderly patients with ischemic stroke fatigue were significantly elevated and it has obvious corre-lation .
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Objective To get the knowledge of the diagnosis and treatment of acute pulmonary embolism (APE)in primary hospital.Methods The clinical data of the patients diagnosed with APE were retrospectively reviewed.The patients were clarified into different risk-group by revised Geneva score and Wells score according to the clinical records.Results 17 patients were diagnosed with APE in this time slot,in which male 10 cases and female 7 cases,average age was (51.8 ±18.4)years old,and among them,4 cases with 4 scores of revised Geneva score,9 cases with 4 to 10 scores,4 cases with more than 11,6 cases with less than 4 Wells score,11 cases with more than 4,2 cases with low risk and 15 cases with intermediate risk.The length of hospital stay was (10.9 ±5.4)days in average.In this group,one patient was dead,seven cases improved,six cases remarkably improved and three cases were recued.Fourteen patients received anticoagulation agents and three cases without any.Eleven patients were given thrombolystic therapy,one case was operated and six cases were given interventional treatment.Conclusion Clinicians know APE and keep alert gradually.However,it should be improved in respects of treating and following the APE patients.
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Objective To investigate concentration of plasma neutrophil gelatinase -associated lipocalin ( NGAL) and its clinical significance in ICU populations with sepsis.Methods Fourteen ICU patients diagnosed with sepsis and twenty-four patients with non-sepsis were enrolled in the study,and seventeen healthy adults were selected as healthy control.Blood samples were drawn from patients to measure NGAL, and Acute Physiology and Chronic Health Evaluation ( APACHE ) II and Sequential Organ Failure Assessment ( SOFA ) were performed, the outcome was recorded.Plasma NGAL concentration was measured by enzyme -linked immunosorbent assay ( ELISA) .Results On admission to ICU, the pNGAL concentrations of septic and non -septic patients were (131.4 ±116.4)ng/mL and (48.7 ±30.8)ng/mL respectively,which of healthy control was (39.07 ±12.74)ng/mL.The pNGAL was significantly higher in sepsis than non-sepsis and healthy control ( t=-3.280,3.313,P=0.003,0.021 respectively).Otherwise,no differences of white blood cell (WBC) count [(12.6 ±5.7) ×109/L vs (15.6 ±5.8) ×109/L] and SOFA scores [(7.7 ±3.3)points vs (8.6 ±3.4)points] were found in septic and non-septic patients (t=-1.554,-0.802,P=0.129,0.428).On the admission to ICU,areas under the receiver oper-ating characteristic curves ( ROC curves) of NGAL and WBC for predicting sepsis were 0.740 [95%confident inter-val(CI) 0.566,0.913,P =0.015] and 0.345 ( 95%CI 0.158,0.533,P =0.116) respectively.If pNGAL of 99.4ng/mL as the threshold for predicting sepsis, the sensitivity and the specificity was 50.0% and 85.2%. Conclusion The concentrations of NGAL in septic patients significantly increased compared with that in non-sepsis and in healthy adults.And the NGAL levels maybe another sensitive biochemical marker for predicting infection.