ABSTRACT
Appropriate culture media and environment for embryonic cell growth considered to be major factors for generation of an embryo in vitro. In this study two embryonic growth condition of Glm and Ham's F-10 + Maternal Serum [MS]%10 were compared. This investigation was prospectively performed on 100 infertile couples that were treated by IVF-iCSL The participants were divided into two equal groups. Ovules obtained from first group were treated with Ham's F[10] + Maternal Serum%10 culture media and the second group with Gl[III]. The variables affecting ovule and embryonic growth were measured, and collected data were analyzed by SPSS. The mean of age for tested ladies was 28.01 and the mean for duration of infertility was 6.87 years. The number of pronucleous with moderate quality were 2.14 in-group I and 3.22 in-group 11 [P=0.023]. The number of embryo with three cells were 1.26 and 0.54 and for grade B embryonic cell were 1.18 and 1.78 and grade C embryonic cell were 1.08 and 0.56 in group I and II respectively [p=0.29]. The mean of grade B blastomer in GI[III] media was more than Ham's F[10]+ Maternal Serum 10%. Compairing the means; the mean of grade B blastomers in Glm media was shown to be more than Ham's F[10] + Maternal Serum 10%. All of the findings showed that Gl[III] cultural condition is more effective than Ham's F[10] and the maternal serum as a supplement has no considerable impact
Subject(s)
Humans , Male , Female , Infertility , Culture Media , Embryo Culture Techniques , Mothers , Prospective StudiesABSTRACT
In infertility without suffering tubal factor, WI is the choice therapeutic method. Some studies recommended double IUI for every cycle. Since double IUI needs more laboratory materials and more expenses, so in this study it was tried to evaluate pregnancy rate in infertile couples after single and double IUI. This clinical trial study was performed on infertile couples referred to Fatemeh Alzahra or Babol Clinic hospital for IUI cycles. Patients divided into two groups. In the first group, 36 hours after HCG injection, IUI was done as a single protocol and in the other group; IUI was done 24 and 72 hours after HCG injection as double protocol. Then data were analyzed by fisher's exact test. Two hundred and fifteen women participated in our study and their mean age was 26.02 +/- 5.5 and the mean [ +/- SD] duration of infelicity was 3.73[ +/- 2.75] years. In the first group, pregnancy rate was 16.9% and in second group it was 19.6% without any significant difference between two groups. Also, there was no significant difference between groups in pregnancy rate with different etiology of infertility. Some studies recommended that double IUI is more successful than single IUI. But present findings suggest that there is no difference in pregnancy rate between single IUI and double IUI
ABSTRACT
Hinosan and Diazinon are organophosphorate pesticides with contacting and penetrating effect that are used in agriculture against rice blast. Therefore, with regard to high use of these pesticides in the north of Iran, their harmful effects and high rates of infertility that are increasing every year in Mazandaran province. This study was performed to determine the effects of Hinosan and Diazinon on sperm parameters. This experimental study was performed on sperm samples of subjects who referred for semen fluid analysis [SFA]. The sperms were affected by Hinosan and Diazinon [0.001 and 0.0001 concentration]. The sperm number, motility and grading at the times of 0, 1, 2 and 4 were recorded. Data were analyzed by repeated measurement and compared with normal condition. Hinosan and Diazinon can reduce sperm motility by time [P<0.05], but the increase of dose had not significant effect on sperm motility decreasing. During the study, changes in increasing of grade I and grade II was statistically significant [P<0.05], but these changes were not significant in grade III. According to the findings, Hinosan and Diazinon can exert their reducing effects on number and motility of sperm as a time dependent profile. These effects are not dependent to the dose of the materials