Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.

Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.</p><p><b>METHOD</b>The HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (5:95) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.</p><p><b>RESULT</b>The effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.</p><p><b>CONCLUSION</b>It has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.</p>

Clinical Analysis of Human Papillomavirus in Paraffin-embedded Cervical Lesion Tissues by Gene Clip Technology / 中华医院感染学杂志

OBJECTIVE To investigate the clinical significance of human papillomavirus in paraffin-embedded cervical cancer and precancerous lesion tissue by gene clip technology.METHODS 153 Patients with paraffin-embedded examples.DNA was extracted from paraffin-embedded tissues and amplified by polymerase chain reaction(PCR).RESULTS The positive rate of high-risk HPV of inflammation was 8.33%,CINⅠ45.83%,CINⅡ/CINⅢ 87.50% and invasive cancer 92.21%.The HPV infection rate of squamous cell carcinoma was 94.12%.The HPV infection rate of adenocarcinoma was 88.46%.Among all the patients with cervical cancer and CIN,the infection rate of HPV16,the most genotype,was 88.98%.The infection rate of HPV18,the second most subtype,was 33.06%.In addition,the minority were infected HPV52、33、59、68.Among 48 cases of cervical squamous cell carcinoma,the infection rate of HPV16,HPV18 was 93.73% and 27.08% respectively.Among 23 cases of cervical adenocarcinoma,the infection rate of HPV16,HPV18 was 82.61% and 52.17% respectively.On the other hand,all the patients with cervicitis were HPV single infection.The HPV multiple infection rate of CINⅠ,CINⅡ/CINⅢ,cervical cancer was 20.00%,28.57%,36.62% respectively.CONCLUSIONS Gene chip technology can detect multiple HPV genotypes in paraffin-embedded tissues with high sensitivity and specificity,which is useful in the pathogenesis and prevention of cervical cancer.

Poor prognosis associated with vascular endothelial growth factor(VEGF) and platelet drived endothelial cell growth factor(PD-ECGF) expression in aged patients with gastric carcinoma / 中华老年医学杂志

Objective To investigate the expression of vascular endothelial growth factor (VEGF) and platelet drived endothelial cell growth factor (PD-ECGF) and their influence on the clinicopathological characteristics and the prognosis in aged patients with gastric carcinoma. Methods The expression of VEGF and PD-ECGF were examined with immnunohistochemical technique through preoperative biopsied specimens in 92 cases. Results The expression of VEGF and PD-ECGF were significantly higher in gastric carinoma than in chronic atrophic gastritis. VEGF and PD-ECGF levels in advanced carcinoma were higher compared with early carcinoma( P

Clinicopathological analysis of loss of fragile histidine triad expression in lung cancer / 中国肺癌杂志

<p><b>BACKGROUND</b>To investigate the relationship between expression of fragile histidine triad gene protein, Fhit, and clinicopathological characteristics of human lung cancer.</p><p><b>METHODS</b>Fhit protein expression was detected in 92 cases of formalin-fixed, paraffin-embedded human lung cancer by citrate-microwave-SP immunohistochemical method, of which 52 were non-small cell cancer (NSCLC) and 40 small cell cancer (SCLC). Its relationship to histological grade, lymph node metastasis and histological classification were analysed.</p><p><b>RESULTS</b>The loss of Fhit protein expression were found in 62.0% (57/92) and 4.3% (4/92) of cancer tissue and normal lung tissue, respectively and there was a significant difference in the expression of Fhit protein between cancer and normal tissue (P=0.000). 53.8% (28/52) of the cases of NSCLC showed a marked loss or absence of Fhit expression, 46.2% (24/52) of cases were negative, 7.7% (4/52) showed a higher expression and 38.5% (20/52) equal to the level of Fhit expression compared with the matched normal tissues. The loss of Fhit expression was closely related to histological grade (P=0.003), to lymph node metastasis (P=0.029), and to histological classification of the cases (P=0.003). There was a significant difference between grade I+II (38.2%; 13/34) and grade III cancer (83.3%; 15/18), between cancers with lymph node metastasis (70.8%; 17/24) and those without (39.3%; 11/28), and between squamous cell carcinoma ( 68.6%; 24/35) and adenocarcinoma (23.5%; 4/17). The loss of Fhit protein expression was found in 33 of 40 cases of SCLC (82.5%) and the remainder 7 cases (17.5%) showed the same quantity of expression of Fhit, compared with the normal bronchial mucosa.</p><p><b>CONCLUSIONS</b>The expression of Fhit protein may be associated with the decreasing differentiation, lymph node metastasis and histological classification in NSCLC, and be corresponding to the occurrence and evolution of SCLC. These results suggest that the decreased Fhit expression plays an important role in the development and progression of the tumor, and thus may become a new prognostic marker in human lung cancer.</p>