Factors affecting poor eyesight among primary and middle schoolstudents in Tongzhou District / 预防医学

Objective@#To investigate the prevalence and influencing factors of poor eyesight among primary and middle school students in Tongzhou District, Beijing Municipality, so as to provide the evidence for developing control strategies for poor eyesight among primary and middle school students.@* Methods@#Grades 3 to 6 students in district- and township-level primary schools, grades 1 to 3 students in district- and township-level junior high schools, and grades 1 to 3 district- and township-level high schools were sampled in Tongzhou District using the stratified cluster sampling method from 2020 to 2021. Basic information, daily activity, sleep duration and eye-using habits were collected using the specific questionnaires for poor eyesight and influencing factors among students in the 2018 national program for common diseases and health risk factors surveillance program among Chinese students, and the height and body weight were measured. Factors affecting poor eyesight were among primary and middle school students identified using a multivariable logistic regression model. @*Results@#A total of 771 valid questionnaires were recovered, and the respondents included 392 male students (50.84%) and 379 female students (49.16%), and 321 primary school students (41.63%), 228 junior high school students (29.57%) and 222 high school students (28.79%). The prevalence of poor eyesight was 73.54% among the respondents. Multivariable logistic regression analysis showed that education phase (junior high school, OR=2.940, 95%CI: 1.931-4.476; high school, OR=5.998, 95%CI: 3.701-9.723) , obesity (OR=1.989, 95%CI: 1.258-3.146), daily exercise duration of less than 1 h (OR=1.931, 95%CI: 1.351-2.760), daily sleep duration of less than 8 h (OR=1.719, 95%CI: 1.193-2.477), at least 33 cm distance between a reading book and eyes (sometimes, OR=2.165, 95%CI: 1.320-3.550; never, OR=2.634, 95%CI: 1.767-3.928) and continuous short-distance eye use duration of 1 h and longer (OR=1.455, 95%CI: 1.020-2.078) were associated with poor eyesight among primary and middle school students.@*Conclusions@# The prevalence of poor eyesight is high among primary and middle school students in Tongzhou District. Higher grade, obesity, inadequate exercise and sleep duration and poor eye-using habits may increase the risk of poor eyesight.

Advancement of imaging technology for coronary microcirculation dysfunction assessment / 生物医学工程学杂志

Coronary microcirculation dysfunction (CMVD) is an important risk factor for the prognosis of re-perfused ischemic heart. Recent studies showed that the evaluation of CMVD has significant impact on both the early diagnosis of heart diseases relevant to blood supply and prognosis after myocardial reperfusion. In this review, the definition of CMVD from the perspective of pathophysiology was clarified, the principles and features of the state-of-the-art imaging technologies for CMVD assessment were reviewed from the perspective of engineering and the further research direction was promoted.

Effect of atorvastatin on the apoptosis of human umbilical vein endothelial cells and its drug mechanism

Recent studies have shown that statins can inhibit the apoptosis of vascular endothelial cells. Many Pharmacological actions of statins have nothing to do with thier cholesterol lowering effects. These effects are called non lipid lowering cardiovascular protective effects. In this study, by establishing a high glucose induced vascular endorhelial cell apoptosis model, we explored the mechanism of the non lipid - related cardiovascular protective effect of atorvastatin. The results showed that atorvastatin could protect human umbilical vein endothelial cells from damage induced by new high glucose and inhibit apoptosis. High concentration atorvastatin can up regulate the expression of Bcl-2 and down regulate the expression of Baxprotein [P<0.05]. This suggests that statins may play a role in cardiovascular protection by inhibiting endothelial cell apoptosis. This results is confirmed by experiments, which can provide clues for clinical treatment, and combine drug therapy and lifestyle intervention to reduce blood sugar

Pros and cons of probiotics in immunocompromised individuals / 中华全科医师杂志

Probiotics as intestinal flora regulators are increasingly applied in clinical practice;however,the administration of probiotics in immunocompromised individuals is rarely reported.This article is aimed to review the benefits and risks of probiotics in immunocompromised individuals,such as HIV-infected individuals and patients with malignant tumors.The research evidence shows that probiotics can reduce the occurrence of different types of diarrhea,regulate the intestinal immune status and decrease the incidence of complications in immunocompromised patients.Randomized controlled clinical trials have shown that the risk of adverse events is not significantly increased in immunocompromised patients compared to those with intact immune status and the probiotics-related infections are only presented in case reports.

Anti-oxidant Effect of Gastrodin in Epilepsy Rats / 昆明医科大学学报

Objective To explo the antioxidant effect and molecular mechanism of gastrodin (Gas) in epilepsy (EP) rats induced by LiCl-pilocarpine (PILO) . Methods Eighty male SD rats were randomly divided into 5 groups: sham group, EP group, therapy groups (pretreated with 60 mg/kg, 90 mg/kg, 120 mg/kg of gastrodin respectively) . The EP model was esteblished by peritoneal injection of LiCl-PILO. Therapy groups were pretreated with various concerntration of Gas. The control group was given the same dosage of normal saline. The alteration of behavior was observed, the concentration of catalase (CAT), glutathion (GSH), superoxide dismutase (SOD), glutathion reductase (GR), total antioxidtion (T-AOC) and malondialdehyde (MDA) in rats brain cortex were detected by chemical colorimetric method, phosphorylation of p38 was determined by western blot. Results There was no EP seizure in sham group,and the EP seizure degree in therapy groups (gas pretreated groups) was significantly decreased,and had statistically significant difference with EP group (P<0.05) . The EP model rats exhibited a significant decrease in the concentration of endogenous antioxidants (CAT, GSH, SOD, GR and T-AOC), while an increase of the concentration of MDA and phosphorylation p38 protein as compared to sham group (P<0.05) . After treatment of the Gas,treatment group rats attenuated the seizure degree,exhibited a significant increase of the concentration of endogenous antioxidants (P<0.05),while a decrease in concentration of MDA and phosphorylation of p38 as compared to model group (P<0.05) . Conclusion Gas may have a neuroprotective role in central nervous system of epileptic rats modle by down-regulateing the seizure degree and the activity of p38 kinase and up-regulateing the content of endogenous antioxidants.

The Activation and Polarization of Microglia in Epileptic Rats Induced by Pilocarpine / 昆明医科大学学报

Objective To explore the activition and polarization of microglia in the epileptic rats induced by lithium chloride-pilocarpine. Methods One hundred male SD rats were randomly divided into five groups: control group and different time points model groups including 1d,3d,7d and 14d. Epilepsy models were established by lithium chloride-pilocarpine intraperitoneal injection. The control group was given the same dosage of normal saline. The morphology change was detected by immunofluorescence,and the expressions of iNOS and Arg-1 were determined by IHC at respective time points. Results Compared the model groups with control group,microglia was activated,synapsis was shorten,volume got bigger,most of them seemed as amoebocyte,the expression of iNOS increased and Arg-1 decreased,especially at 3d.ConclusionThe results from this study indicated that microglia was activated and polarized in epileptic rats induced by pilocarpine.

Evaluation of anticancer efficacy of dihydromyricetin in gastric cancer cells SGC7901 / 重庆医学

Objective To investigate the anticancer efficacy of dihydromyricetin (DMY)in gastric cancer cells SGC7901. Methods The DMY was puritied using RP-HPLC was almost 98%.The cell viability was assessed by CCK-8 Assay.The cell ap-optosis was assessed by flow cytometry.Using ultrasonic extraction with neutral water and reversed-phase high-performance liquid chromatography,DMY was purified from rattan tea.Results By using RP-HPLC,the purified DMY is almost 99%.DMY inhibi-ted the growth of tumor cells in the low concentration(≤ 1 mmol)with a dose-dependent manner(P <0.05 ).Further study on DMY showed that it induced apoptosis in SGC7901 cell line with a dose-dependent manner.Conclusion DMY is a potential thera-peutic agent for gastric cancer.

Antioxidant activities in vitro and in vivo of water-soluble polysaccharide isolated from Sparganium stoloniferum Buch. Ham

In this study, the crude polysaccharides from Sparganium stoloniferum Buch.-Ham were prepared using hotwater extraction and further deproteinzated by Sevage method. The purified fraction of crude polysaccharide was obtained using a DEAE-52 cellulose chromatography and named with WSSP. Then, the antioxidant capacities of WSSP were assessed in vitro and in vivo. The results in vitro indicated that the WSSP possessed notably free radical scavenging capacity. And the antioxidant abilities were dose-dependent and increased with increasing dose of sample. The findings in vivo showed the gavage administration of WSSP can increase SOD and TAOC activities, and decrease MDA levels in tissue and serum of mice. Therefore, the WSSP may serve as a potential antioxidant

5-azacytidine induces apoptosis of bone marrow mesenchymal stem cells in mouse / 中华实用儿科临床杂志

Objective To investigate the effect of 5-azacytidine(5-AZA) on apoptosis of bone marrow mesenchymal stem cells(BMSCs).Methods BMSCs were isolated from bone marrow of mouse tibia and femur; the expression of MSC specific markers CD44 and CD90 in BMSCs was measured by immunofluorescence staining;BMSCs were cultured in vitro in the medium supplemented with 0,10 and 20 μmol/L 5-AZA for 48 hours.Cell apoptosis was measured with fluorescent labeled inhibitor of caspases (FLICA) apoptosis kit and 4',6-diamidino-2-phenylindole (DAPI) staining ;the expression of apoptosis-related proteins Annexin V and Caspase-3 in the treated BMSCs was detected by Western blot.Results In this study,BMSCs positively expressed MSC specific markers CD44 and CD90.DAPI staining and Caspase-3 staining both showed that 10 and 20 μmol/L 5-AZA markedly increased apoptotic rate of BMSCs;the apoptosis-positive rate in DAPI staining was (21.086 ± 2.601) ％,(34.467 ± 3.724) ％ and (46.512 ± 3.864) ％,the apoptosis-positive rate in Caspase-3 staining was (5.354 ± 0.735)％,(15.462 ± 2.385)％ and (28.190 ± 4.190)％ in the controls,10 and 20 μmol/L 5-AZA groups,and there were significant differences among the control group and 5-AZA treated groups(all P ＜0.01).Western blot assay showed that Annexin V and Caspase-3 were both markedly upregulated in 5-AZA treated cells;the relative level of Annexin V expression was(26.612 ±2.184)％,(42.873 ±4.313)％ and (50.056 ± 4.457) ％,the relative level of Caspase-3 expression was (19.231 ± 2.683) ％,(38.618 ± 5.385) ％ and(91.235 ± 7.116)％ in the controls,10 and 20 μmol/L 5-AZA groups,and there were significant differences among the control group and 5-AZA treated groups (all P ＜ 0.01).Conclusion The commonly used doses of 5-AZA can induce apoptosis of BMSCs.

RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities

RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain. RNF152 is localized in lysosomes and co-localized with LAMP3, a lysosome marker. Moreover, over-expression of RNF152 in Hela cells induces apoptosis. These results suggest that RNF152 is a lysosome localized E3 ligase with pro-apoptotic activities. It is the first E3 ligase identified so far that is involved in lysosome-related apoptosis.

Effect of carboxymethyl-chitosan on proliferation and collagen synthesis in keloid fibroblasts in vitro / 中华医学美学美容杂志

Objective To investigate the effects of carboxymethyl-chitosan (CM-CH) on keloid fibroblasts (KFB) proliferation and collagen synthesis. Methods Keloid fibroblasts were isolated from fresh keloid tissue and cultured. The effect of CM-CH on proliferation was examined by MTT assay. The synthesis of collagen was evaluated by hydroxy proline (HP) colorimetric analysis. Results The fibroblasts were treated with CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml 48h and 72h after treatment, and all of the three concentrations showed inhibitory effects significantly (P0.05) at 200 ?g/ml concentration after 24h, 48h, and 72h. CM-CH at concentration of 10 ?g/ml, 50 ?g/ml, and 100 ?g/ml after treatment for 48h on KFB could markedly decrease the synthesis of collagen (P

The Protective Effects of Nitroxides Against Oxidative Damage to Human Keratin ocytes / 中华皮肤科杂志

Objective To study the protective effects of nitroxides against hu man keratinocytes oxidative damage induced by H2O2 and its possible mechanisms. Methods Normal human keratinocyte cultures obtained from foreskin were served as test-system. Human keratinocytes were cultured in human keratinocytes growth m edia (KGM) without serum and supplemented with 0.1 mmol/L Ca2+. Experiments wer e performed in culture when the cells grew to fuse. Oxidative damage was induced by adding H2O2 directly to the culture media at different concentrations in the present and absence of nitroxides [(2, 2, 6, 6-tetramethylpiperidine (TEMPO) a nd 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine (TEMPOL)]. The cell viability w as monitored and the intracellular level of reduced glutathione (GSH), the activ ities of glutathione-peroxidase(GSH-Px), superoxide dismutase (SOD), and catal ase were evaluated. Results ①H2O2 could cause damage to human keratinocytes dir ectly. There was a significantly negative correlation between H2O2 concentration and cell viability. ②The level of GSH in keratinocytes lowered with treatment of H2O2. The activities of GSH-Px, SOD and catalase decreased. ③Pretreatment o f the cells with TEMPO and TEMPOL inhibited the damaging effects of H2O2 on cell viability and on cell antioxidant enzymatic systems. Conclusion The results of the study suggest that nitroxides may provide protection for cultured human kera tinocytes against H2O2-induced oxidative injury. It is proposed that the effec ts of nitroxides against cell oxidative damage be related to their protection of cellular enzymatic activities and maintaining cellular antioxidant systems.