ABSTRACT
Objective:To develop a quantitative HPLC-MS/MS method for the determination of astragulin and kaempferol in Bairui granule. Methods:A column of Ultimate XB-C C18(100 mm×2.1 mm,5 μm)was used. The mobile phase was composed of 0.1% formic acid-water(A) and 0.1% formic acid-acetonitrile(B) with gradient elution. The flow rate was 0.3 ml·min-1. The detection was performed with multiple teactions monitoring (MRM) using electrospray ionization (ESI). The precursor/product ion transitions were monitored at m/z 447.2→m/z 284.0 (anion mode) for astragulin and m/z 285.1→m/z 187.1 (anion mode) for kaempferol. The temperature of column was set at 40℃. Results:A good linearity was obtained with the concentration ranging from 9.86 to 1 970 ng·ml-1(r=0.999 5) for astragulin and 8.73 to 437 ng·ml-1(r=0.999 6) for kaempferol. The average recovery of the two inves-tigated compounds at low, medium and high concentrations was 99.23%, 99.65% and 99.23%, and 98.24%, 99.13% and 99.69% with the RSD of 1.84%,1.37% and 1.21%,and 1.38%,0.96% and 1.47%, respectively (n=6). Conclusion: The method is rapid,accurate and reliable,which can be used to control the quality of Bairui granule.
ABSTRACT
OBJECTIVE@#To determine the expression of MTA1 and RECK gene in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathological features.@*METHOD@#In situ hybridization was used to detect the expression of MTA1 and RECK mRNA in 60 cases of primary NPC (30 cases with, 30 cases without cervical lymph node metastasis), 10 cases of metastatic lymph node (MLN) and 20 cases of chronic nasopharyngitis tissue (CNT).@*RESULT@#1) Positive rates of MTA1 mRNA expression in CNT, NPC and MLN were 30.0% (6/20), 71.7% (43/60) and 80.0% (8/10), respectively. The positive expressions of MTA1 in NPC and MLN were significantly higher than in CNT (P0.05), while they had positive relationship with lymph node metastasis, tumor recurrence and the post-treatment 5-year survival periods of NPC (P<0.05). The expression of RECK mRNA was negatively correlated with the expression of MTA1 mRNA in NPC (r = - 0.541, P<0.05).@*CONCLUSION@#These data suggest that the aberrant expression of MTA1 and RECK gene may be involved in the invasion and metastasis of NPC. They may be good biomarkers for evaluating the cervical lymph node metastasis, recurrence and prognosis of NPC.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma , Genetics , Pathology , GPI-Linked Proteins , Genetics , Histone Deacetylases , Genetics , In Situ Hybridization , Lymphatic Metastasis , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Genetics , Pathology , Neoplasm Staging , Repressor Proteins , GeneticsABSTRACT
OBJECTIVE@#To investigate the expression and the relativity of RECK,RAGE and MMP-9 in nasopharyngeal carcinoma (NPC) tissues.@*METHOD@#RECK, RAGE and MMP-9 were detected with immunohistochemical methods in 64 NPC specimens(30 cases with cervical lymph nodes metastasis, while the other 34 cases are not) and 30 specimens with chronic nasopharyngitis.@*RESULT@#RECK hardly expressed in chronic nasopharyngitis and NPC tissues, However, strong expression of RECK could be seen in the surrounding inflammatory cells and matrix of cancer nests. The positive rates of RECK in NPC with and without cervical lymph nodes metastasis were 3.3% (1/30) and 11.8% (4/34), separately, There was no statistical significance between the groups (P > 0.05). The positive rates of RAGE in chronic nasopharyngitis and NPC tissues were 100% (30/30) and 70.3% (45/64) respectively, while MMP-9 were 46.7% (14/30) and 78.1% (50/64). The positive rates of RAGE in NPC with and without cervical lymph nodes metastasis were 86.7% (26/30) and 55.9% (19/34) separately, whereas MMP-9 were 90.0% (27/30) and 67.7% (23/34) respectively. They all showed statistical significance between the groups. MMP-9 had a negative correlation with RECK (r = -0.369, P < 0.05) and a positive correlation with RAGE in NPC (r = 0.471, P < 0.05).@*CONCLUSION@#The expression of RECK and RAGE in NPC were down-regulated, the expression of MMP-9 was up-regulated. While the expression of RECK in NPC with cervical lymph nodes metastasis was down-regulated, while RAGE and MMP-9 were up-regulated. The abnormal expression of the three genes may be related to the progression of NPC. RECK and RAGE may be involved in the invasion and metastasis of NPC by regulating the expression of MMP-9.