ABSTRACT
Objective:To investigate the clinicopathological features and prognosis of medullary thyroid carcinoma (MTC) co-existent with Hashimoto's thyroiditis (HT).Methods:Seven cases of MTC co-existent with HT were collected from Fujian Cancer Hospital between Jan 2007 and May 2021. Its clinicopathological features were analyzed.Results:All patients were adult female with a median age of 49 years, and 6 of them were unilateral MTC. The tumor diameter ranged from 0.4 to 7.0 cm. The thyroid tissue surrounding the tumor showed HT. The follow-up time was 16 to 152 months, with a median time of 61 months, 5 patients (tumor diameter <1 cm) were biochemically cured, 1 patient (tumor diameter >1 cm) was anatomically cured. The another patient died due to tumor progression 30 months after surgery, the tumor cells in which were highly atypia and more mitoses (5~8/10HPF) necrosis and vascular invasion were also observed.Conclusion:MTC with HT are mostly sporadic cases, and most of them have a fair prognosis.
ABSTRACT
Objective To identify the role of FOXC2 in the invasion and migration of colorectal cancer cells .Methods Stable cell lines expressing FOXC2(SW480/FOXC2) or vector (SW480/pBabe) were established using retroviral infection method .The morphology alterations of SW480 cells were observed using a microscope .Western blot analysis and immunofluorescence staining assays were performed to detect the expression of E‐cadherin ,Vimentin and N‐cadherin .The invasive and migratory abilities of colorectal cancer cells evaluated using Transwell invasion chamber experiment detection .Results The morphology of SW480 cells was significantly changed after overexpression .From the original shape typical of epithelial cells became spindle shaped growth , similar to the morphology of fibroblasts .Western blot analysis and immunofluorescence staining displayed that overexpression of FOXC2 led to significant downregulation of the epithelial marker E‐cadherin ,but upregulation of the mesenchymal markers Vimen‐tin and N‐cadherin .Transwell assay reveals that overexpression of FOXC2 strongly enhanced the migratory and invasive ability of SW480 cells .Conclusion FOXC2 induces epithelial‐mesenchymal transition and promotes the invasive ability of colorectal cancer cells .
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer.</p><p><b>METHODS</b>Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays.</p><p><b>RESULTS</b>SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05).</p><p><b>CONCLUSION</b>The expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.</p>