ABSTRACT
OBJECTIVE:A reversed-phase high performance liquid chromatography(RP-HPLC)method was established to study the pharmacokinetics of cetirizine hydrocloride tablet in 11 healthy male volunteers METHODS:Waters HPLC instrument was used with the Waters symmetry C18 steeless column(3 9mm?150mm,5?m) The mobile phase was composed of acetonitrile-0 02mol/L sodium hydrogen diphosphate-triethylamine(50∶50∶0 16,V/V),which contained 4 0mmol/L sodium dodecyl sulphate Flow rate was 1 0ml/min Detection wavelength was 229nm Propafenone was taken as internal standard A single 10mg oral dose of cetirizine hydrocloride tablet was given to 11 healthy male volunteers Cetirizine concentration was assayed in plasma RESULTS:The standard curve of cetirizine hydrocloride was linear in the range of 12 5～800ng/ml The minimum detection limitation was 5ng/ml The extraction recovery was more than 75% A two-compartment open pharmacokinetic model was adapted in cetirizine plasma concentration-time data analysis The main pharmacokinetic parameters were as follows:Cmax=(429 00?108 80)ng/ml,Tmax=(0 91?0 40)h,AUC0～36(calculated by trapezoid method)=(3 312 72?682 39)ng/(h?ml) CONCLUSION:The method was accurate,sensitive and reliable It is applicable to determine the concentration of cetirizine hydrocloride in human plasma;The main pharmaconetic parameters of the domestic cetirizine hydrocloride tablet were similar to those reported at home and abroad,so it could be extensively used in clinic
ABSTRACT
OBJECTIVE: To develop a method for the determination of levodopa in human plasma and study aarodopa's pharmacokinetics. METHODS: Experiments were performed on Waters 2010 HIPLC system with a 474 fluorecence detector (?EX = 278nm, ?Em = 325nm) and a Lichrospher 100 C18 column. The mobile phase(pH3. 7) was 90% water containing 0.08 mmol/L EDTA, 70mmol/L KH2PO4, 2. 08mmol/L sodium heptanesulfonate and 10% methanal. The flow rate was 1. 0ml/min. The plasma sample was directly injected for determination after being deproteinized with perchloric acid. RESULTS: The linear range was 0. 125-4.0ug/ml,the limitation of detection was 0. 25ug/ml,within- day RSD
ABSTRACT
OBJECTIVE:To established a method for determining matrine and oxymatrine in human plasma.METHODS:The plasma was extracted with chloroform-n-butyl alcohol(98∶2) after basification and purified with neutral alumina solid-phase extraction.Then it was eluted with a Lichrosorb-NH2 column and CH3CN-CH3CH2OH-H3PO4(80∶10∶8),detected at ? 220nm.RESULTS:The linear range was 1.25mg～40mg/L and the limit of detection was 0.1mg/L(S/N=2).The average recoveries of matrine and oxymatrine were 106.96% and 105.04%,respectively.RSDs of within-day and between-day were lower than 13% and 7% respectively.CONCLUSION:The present study provides a simple and reliable method for determining concentrations of matrine and oxymatrine in human plasma.