ABSTRACT
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
Subject(s)
Humans , Biocompatible Materials , Pharmacology , Cell Adhesion , Cell-Matrix Junctions , Cells, Cultured , Dental Implants , Periodontal Ligament , Cell Biology , Surface Properties , Time Factors , Titanium , PharmacologyABSTRACT
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
Subject(s)
Biocompatible Materials/pharmacology , Cell Adhesion , Cell-Matrix Junctions , Cells, Cultured , Dental Implants , Periodontal Ligament/cytology , Surface Properties , Time Factors , Titanium/pharmacologyABSTRACT
Objective To analyze the reasons of the occlusal disorder after mistaken concrescence of maxillofacial fracture, to observe the effect of the physiotherapy for the masticatory movement and the masticatory efficiency after occlusal reconstruction with fixed prosthesis. Methods The reasons of occlusal disorder of 45 patients were analyzed by reviewing the case history and clinical examination. Ultrasound diathermy and laser irradiation were used to help improve masticatory movement. The fixed prosthesis was used to reconstruct the occlusal relationship. Masticatory efficiency was measured with the method of sift out before treatment, one month and one year after treatment. Results The main reason of occlusal disorder was the delay of treatment because of the other treatment of body after trauma. The physiotherapy was effective in improving the masticatory movement. Statistically analysis showed the significantly difference before and after occlusal reconstruction with fixed prosthesis ( P