ABSTRACT
Objective: To investigate the expression of zinc finger with KRAB and SCAN domains 3 (ZKSCAN3) in breast cancer tissues and its clinical significance. Methods: The expression of ZKSCAN3 in 120 specimens of human breast cancer tissues and 60 specimens of adjacent para-cancerous tissues was detected by immunohistochemistry, and its correlation with clinicopathological parameters and overall survival was analyzed. The real-time fluorescent quantitative PCR and Western blotting were used to further detect the expressions of ZKSCAN3 mRNA and protein in breast cancer tissues and the adjacent para-cancerous tissues. Results: The positive expression rate of ZKSCAN3 protein in breast cancer tissues [74% (89/120)] was higher than that in the adjacent para-cancerous tissues [37% (22/60)] (P < 0.001). The expression of ZKSCAN3 protein was correlated with tumor size, lymph node metastasis, tumor differentiation and clinical stage of breast cancer (all P < 0.05). The overall survival rate of patient with ZKSCAN3-positive breast cancer was significantly lower than that of patient with ZKSCAN3-negative breast cancer (P = 0.040). The expression levels of ZKSCAN3 mRNA and protein in breast cancer tissues were higher than those in the adjacent para-cancerous tissues (both P < 0.01). Conclusion: ZKSCAN 3 is associated with the occurrence, progression and metastasis of breast cancer, so it may be an oncogene of breast cancer.
ABSTRACT
Objective:To investigate the expression of transmembrane 4 super family 1 (TM4SF1)in breast cancer tissue,and to elucidate its clinical significance and explore the related molecular biological mechanisms. Methods:A total of 190 cases of human breast cancer,110 cases of paracancerous tissue and 110 cases of normal breast tissue were collected.Immunohistochemistry was used to detect the expression levels of TM4SF1 mRNA in breast cancer tissue,paracancerous tissue,and normal breast tissue;Western blotting method was used to detect the expression levels of TM4SF1 in breast cancer tissue,paracancerous tissue,and normal breast tissue;RT-PCR method was used to detect the expression levels of TM4SF1 mRNA in breast cancer tissue,paracancerous tissue, and normal breast tissue.The positive expression rates of TM4SF1 in breast cancer tissue of the breast cancer patients with different clinicopathological features were detected.Results:The positive expression rate of TM4SF1 in the breast cancer tissue was significantly higher than those in paracancerous tissue and normal breast tissue (P <0.05);there was no significant difference in the positive expression rates of TM4SF1 between paracancerous tissue and normal breast tissue (P = 0.531);the expression of TM4SF1 was not correlated with age,but was closely correlated with tumor size,differentiation degree,lymph node metastasis and tumor stage (P <0.05);the positive expression rate of TM4SF1 in basal like breast cancer tissue was higher than those in the other three types of tissues (P <0.05).The results of Western blotting showed that the expression level of TM4SF1 in breast cancer tissue was higher than those in paracancerous tissue and normal breast tissue (P < 0.05 ), but there was no significant difference in the expression level of TM4SF1 between paracancerous tissue and normal breast tissue (P >0.05). The results of RT-PCR showed that the expression level of TM4SF1 mRNA in breast cancer tissue was higher than those in the paracancerous tissue and normal breast tissue (P <0.01);there was no significant difference in the expression level of TM4SF1 mRNA between paracancerous tissue and normal breast tissue (P > 0.05 ). Conclusion:The TM4SF1 is highly expressed in breast cancer tissue. TM4SF1 may affect the occurrence, development and distant metastasis of breast cancer through various mechanisms.TM4SF1 may be a potential target for the treatment of breast cancer.
ABSTRACT
BACKGROUND:Colagen sponges are applied for hemostatic use, wound healing, and residual cavity filing, which have great values in clinical application and scientific research. OBJECTIVE:To investigate the biological properties, biocompatibility and biodegradability of colagen spongesin vivo. METHODS: The spatial structure, pore diameter and porosity of colagen sponges were characterized by scanning electron microscopy. Transmission electron microscopy was used to observe the conformation of colagen sponges. The secondary structure and thermal denaturation temperature of colagen sponges were analyzed by circular dichroism spectrum. Colagen sponges were implanted intramuscularly into the spinal cord of New Zealand rabbits to observe the degradation and absorption and histological changesin vivo. RESULTS AND CONCLUSION: Colagen sponges had porous structure with varying pore sizes ranging 40-150 μm, the mean pore size of 100 μm, the thickness wal of 1 μm, and a porosity of approximately 95.8%. Colagen sponges had a typical porous structure and periodic light and dark zones. The solution of colagen sponges had a weak positive band near 220 nm and an intense negative band near 206 nm, which indicated a classic triple helix. And the secondary structure and thermal stability of colagen sponges were similar to that of liquid colagen. Colagen sponges began to degrade at 4 weeks, and remained 20% at 12 weeks. These sponges had been associated with foreign body response and inflammation within 2 weeks after implantation. With wound healing, inflammatory reactions gradualy reduced and disappeared. During the implantation and degradation of sponges, no significant fibrous capsule formed and no tissue necrosis occurred at implantation site, indicating that colagen sponges have good performance in bioactivity, biocompatibility and degradation.