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Objective:By analyzing the epidemiological characteristics of brucellosis in Shanxi Province, to provide scientific evidence of strategies for effective prevention and control of the disease.Methods:Surveillance data of human brucellosis from "Infectious Disease Report Information Management System" from 2008 to 2017 were statistically analyzed by descriptive epidemiological method. The time, regional and occupational distribution of brucellosis were analyzed.Results:A total of 54 845 cases of brucellosis occurred in Shanxi Province from 2008 to 2017. The average annual incidence was 15.37/100 000, fluctuated from 8.34/100 000 to 23.53/100 000. One death case was reported in 2015. The highest incidence was 23.53/100 000 in 2014 and the lowest reported incidence was 8.34/100 000 in 2017. Regional distribution range relatively focused on the north areas of Shanxi Province, the number of reported cases of Datong City was the largest (13 998 cases), accounting for 25.52%. The reported incidence of Shuozhou City was the highest (49.68/100 000). The disease was found each month throughout the year, the obvious incidence peak seasons were between March and July, accounting for 60.11% (32 966/54 845).The disease was most commonly found in 40-64 age groups (64.73%, 35 502/54 845). Occupational distribution of patients was mainly farmers (82.97%, 45 504/54 845).Conclusions:The epidemiology tendency of brucellosis in Shanxi Province is relatively controlled comparing with previous period, but even highly active in some local areas. The epidemic situation has spreading in both scope and population. It is necessary to carry out the propaganda of the protection knowledge of brucellosis in the key groups and enhance the self-protection consciousness of high-risk groups.
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Objective To discuss the effects of Liuwei Dihuang Decoction on expressions of TGF-β1 and complement regulatory protein CR1 of the renal on IgA nephropathy rats.Methods Forty SD rats were randomized into normal group, model group, tripterygium glycosides group and Liuwei Dihuang Decoction group after adaptive feeding for one week. Complex method was used to establish IgA nephropathy model. All administration groups were given relevant medicine for gavage for 4 weeks;urine routine, the number of urine erythrocyte and 24 h urine protein were detected;creatinine, urea nitrogen, total serum protein and serum alburin were detected from blood;the renal pathological changes were observed under light microscope by HE and Masson staining;the expressions of TGF-β1 and CR1 were detected by immunohistochemistry.Results Compared with model group,Liuwei Dihuang Decoction could significantly decrease the proteinuria and hematuria levels and renal fibrosis changes of IgA nephropathy model rats, reduce the expression of TGF-β1, and raise the expression of CR1 (P<0.05).Conclusion Liuwei Dihuang Decoction can delay the renal fibrosis in IgA nephropathy rats through reducing the degree of TGF-β1 and promoting the expression of CR1.
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<p><b>OBJECTIVE</b>To investigate the involvement of immunoreactive-dynorphin A in the inhibitory effect of N-nitro-L-arginine on the morphine physical dependence in rats.</p><p><b>METHODS</b>The rats were rendered dependent on morphine by subcutaneous administration of morphine solution three times daily in a manner of dose increment of 5 mg.kg(-1) for 6 days. The degree of morphine physical dependence was monitored by scoring the abstinence syndromes precipitated by 5 mg.kg(-1) naloxone of the rats. The expression levels of immunoreactive dynorphin A in tissues were determined using a radioimmunoassay.</p><p><b>RESULTS</b>Intraperitoneal injection of 5 mg.kg(-1) N-nitro-L-arginine suppresses most of the withdrawal symptoms of morphine dependent rats. N-nitro-L-arginine can elevate the expression of immunoreactive dynorphin.</p><p><b>CONCLUSIONS</b>Chronic N-nitro-L-arginine administration can inhibit the development of morphine physical dependence in a manner of dose-dependence, which is significantly related to its role of regulating the endogeneous dynorphin system.</p>
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Animals , Male , Rats , Dose-Response Relationship, Drug , Dynorphins , Physiology , Morphine Dependence , Nitroarginine , Pharmacokinetics , Pharmacology , Rats, Sprague-DawleyABSTRACT
Objective To investigate the effect of N-nitro-L-arginine (NO2Arg) on the development of morphine tolerance and dependence.Methods Forty male SD rats weighing (180 ? 18)g were randomly divided into 5 groups for the study of morphine physical dependence and tolerance : group Ⅰ normal saline; group Ⅱ morphine; group Ⅲ morphine + 1 mg?kg-1 NO2Arg; group Ⅳ morphine + 5 mg?kg-1 NO2 Arg ; group Ⅴ morphine +20 mg?kg-1 NO2Arg. Twenty-four male Kunming mice weighing (20.0 ? 2.3)g were randomly divided into 3 groups for the study of psychological dependence: group Ⅵ morphine; group Ⅶ dihydro etorphine; group Ⅷ normal saline. The morphine physical dependence was induced by dose increment. The degree of morphine tolerance was assessed by the pain threshold to heat radiation using tail-flick test. The degree of morphine physical dependence was determined by evaluating the abstinence syndrome precipitated by 4 mg?kg -1 naloxone. The psychological dependence of mice was evaluated by conditioned place preference. Results Consecutive injection of NO2Arg inhibited the development of morphine tolerance in a dose-dependent manner. 5 mg?kg-1 NO2 Arg suppressed most abstinent symptoms of morphine dependent rats. The duration when morphine and dihydro etorphine psychologically dependent mice stayed in the preferred compartment increased significantly from (6.1? 2.0)min and (8.0?0.7)min to (9.3?1. l)min and (9.5 ? 1.2) min by 4 mg?kg-1 NO2 Arg. Conclusion NO2 Arg can inhibit the development of morphine tolerance and dependence and reverse the existing psychological dependence.