ABSTRACT
This study was performed on 57 NHL cases [31 m and 26 f] with different stages, randomly selected from Medical Oncology Department, Minoufiya University hospitals. All patients were subjected to peripheral blood collection, bone marrow aspiration and biopsy to detect IgH and TCR gene rearrangement using PCR technique. Also all samples were examined for morphological changes by H and E stain, immunohistochemistry using CD20, CD 45R and LCA antibodies; The morphological examination showed 6/57 positive results [10.5%] in the bone marrow aspiration, 17/49 [34.7%] in biopsy, 20/49 [40.8%] in combined aspiration, biopsy and the immunohistochemistry stain. By using PCR method, the IgH gene rearrangement was positive in 32/52 [61.5%] using bone marrow aspiration; while 9/24 [37.5%] in peripheral blood and 35/54 [64.8%] in combined bone marrow and peripheral blood using FR-llla, FR-lla, FR l-c, Vu-Ju and their combination. The TCR-y gene rearrangement detects clonality in 9/52 [17.3%] using bone marrow aspiration, while 11/54 [25.9%] in peripheral blood and 14/54 [25.9%] in combined bone marrow aspiration and peripheral blood examinations
Subject(s)
Humans , Male , Female , Bone Marrow/pathology , Bone Marrow Examination , Immunohistochemistry , Polymerase Chain Reaction , Gene RearrangementABSTRACT
Recently, the availability of an anti-core antigen [Ag] monoclonal antibody allowed the development of an enzyme linked immunosorbent assay [ELISA] that showed the presence of hepatitis C virus [HCV] core Ag in peripheral blood of HCV- infected patients. The aim of our study was to determine the specificity and sensitivity of HCV-core antigen assay in serum compared to serum HCV-RNA by qualitative and quantitative PCR in diagnosis of HCV infection in children. Our study was performed on fifty children complaining of acute and chronic liver diseases, attending the pediatric department, National Liver Institute, Menoufiya University from May 2002 to October 2003. They were divided into two groups. Group one consists of 25 children with HCV infection, 20 of them with chronic infection and the other 5 with acute infection. Group two consists of 25 children with liver diseases other than HCV as a control group [to with autoimmune hepatitis, 4 with HBV infection, 4 with hepatosplenic shistosomiasis, 4 with fulminant hepatic failure, 2 with venous outflow block and one with congenital hepatic fibrosis]. In our study HCV core antigen assay showed a specificity of 100 % [25/25] compared to PCR in the diagnosis of HCV infection. However, HCV-antibody testing showed 72 % specificity compared to PCR. While sensitivity of HCV core antigen was 88% among all cases, 80 % [44 in diagnosis of acute HCV cases and 90 % [18/20] in diagnosis of chronic HCV cases in children and showed total validity of 94 % in comparison to that of PCR. The 3 negative cases defected by PCR and not by HCV-core Ag were with low viremia with the highest one being 11000 IU/ml. In comparison, HCV- Ab detected one out of the 5 acute cases and 19 out of the 20 chronic ones. In our study, by applying the ROC curve it was found that the best cutoff value of viremia is 15 X 10[3] IU/ml. At that level of HCV- RNA, the sensitivity and specificity of HCV- core Ag assay are 100 %
Conclusion: HCV core antigen assay may obviate many of the problems encountered with the serologic assay of HCV infection and be used in diagnostic settings as if shows comparable specificity and sensitivity to PCR. Moreover, it is simple, of less cost and not prone to contamination as PCR technique. It is also of special value in the window phase of HCV infection before seroconversion. Further study on a larger number of children with HCV infection is recommended to prove its value and its relation to the level of HCV viremia
ABSTRACT
Coagulation is probably initiated when factor VII or Vll7a in blood gains access to TF at the site of a blood vessel wound. The resulting factor Vlla -TF complex then activates some factors X to Xa and IX to IXa with the initial generation of factor Xa, however the inhibitory properties of TFPl become manifest and inactivate factor Vlla-TF. This study aimed to assess the TFPl level in 25 neonate with clinical and laboratory findings of septicemia and 70 healthy neonates as a control group. There was a significant decrease in TFPl and fibrinogen in diseased group than control [47.4 +/- 78.5 vs 92.7 +/- 77.4 ng/ml and 235.6 +/- 63.7 vs 427.2 +/- 44.5 mg/dl respectively] and increased PTT and CRP in diseased group than control [73 +/- 38.1 vs 26.9 +/- 1.1 and 52.2 +/- 23.3 vs 4.7 +/- 1.0 respectively]
Conclusion: TFPl is decreased in neonatal septicemia mostly due to its consumption due to activation of the coagulation pathway by the tissue factor and there is no correlation between TFPl and CRP, PT and LDL. A large study is needed to assess TFPl in different causes of infection and the possible role of the recombinant TFPl in ameliorating sepsis if possible
ABSTRACT
Objective: Management of splenic injury is a challenging surgical emergency. The spleen has important immunologic functions. Therefore, conservation of the spleen after trauma-whenever possible - is necessary. The present study was carried out to assess the efficacy of topical fibrin sealants [fibrin glue] in comparison with gelatin sponge [gelfoam] and oxidized regenerated cellulose [surgicel] in a rat spleen trauma model
Material and Methods: A standardized traumatic splenic injury was inflicted in male Sprague-Dawley albino albino rats. Animals were divided into four groups [10 rats per group]. Rats that were managed only with surgical gauze served as controls [group 1]. The rest of the animals were categorized as follows; gel foam [group2], surgicel [group 3] and fibrin glue [group 4]. The time necessary to achieve hemostasis was recorded for each rat. Spleens were removed 72 hours later to examine the histopathological parenchymal effects of the topical agents
Results: Complete hemostasis was achieved in all animals within minutes, but fibrin glue and gel foam were significantly superior compared to the control group [p < 0.05]. The surgicel didn't show statistical significance over the control group [p> 0.05]. Moreover, the fibrin glue was significantly better in achieving hemostasis compared to the gel foam [p < 0.05]. Histopathological findings showed that the gelfoam exhibited the most severe inflammatory reaction, while the surgicel caused a moderate inflammatory reaction. Fibrin glue constituted the least inflammatory parenchymal reaction
Conclusion: The present study illustrated that the use of fibrin sealants can be considered an efficient reliable method of attaining hemostasis after splenic injury. Larger prospective clinical studies are recommended
ABSTRACT
Twenty-seven patients with an age range of 36-71 years were included in this study. They were subjected to thorough clinical, radiological and biochemical assessment as well as serological testing for HCV, HBV and serum alpha fetoprotein [AFP] level. Serum vascular endothelial growth factor [VEGF] levels were measured by an enzymeournallinked immunosorbent assay kit. Samples from 15 healthy adults were obtained as controls for the assessment of serum VEGF levels. An immunohistochemical staining was done to study the tumor VEGF expression in the resected specimens. A histologic grade of tumor differentiation was also performed. Prognostic information was obtained by a close follow up every 2-3 months. The study demonstrated that serum VEGF level in HCC patients appears to reflect the disease potential activity for an aggressive behavior and can be used as a predictor of tumor recurrence