ABSTRACT
OBJECTIVE: To establish a micellar electrokinetic chromatography(MEKC) method for simultaneous determination of salidroside, tyrosol, ligustroflavone, specnuezhenide, oleanolic acid, and ursolic acid in Ligustri Lucidi Fructus. METHODS: 60 mmol·L-1 borax-10 mmol·L-1 SDS-30 mmol·L-1 hydroxypropyl-beta-cyclodextrin-10% methyl alcohol was used as the buffer solution (pH 9.03). Uncoated fused silica capillary (75 μm×64.5 cm, 56 cm of effective length) was used with separation voltage of 20 kV. The detection wavelength was set at 210 nm. The column temperature was maintained at 25°C, and the sample was injected at 5 kPa×6 s. RESULTS: The calibration curves of the six index components showed good linearity (r>0.95) in the range of the tested concentrations, and the average recoveries of the method were between 94.57% and 102.07% (RSD<5%). CONCLUSION: The method is simple, accurate and reproducible, and can be used for the quality control of Ligustri Lucidi Fructus.
ABSTRACT
OBJECTIVE: To establish a determination method of five index components to control the quality of Glycyrrhizae Radix et Rhizoma and Longchai Decoction. METHODS: An ODS C18 column (4.6 mm × 250 mm, 5 μm) was adopted; the mobile phase consisted of acetonitrile -0.05% phosphoric acid with gradient elution at the flow rate of 1 mL · min-1; the detection wavelength was 237 nm, and the column temperature was 30°C. RESULTS: The five index components achieved baseline separation, and the negative sample showed no interference. The linear ranges were 0.156 8-1.568 μg for liquiritin (r=0.999 7), 0.157 1-1.571 μg for isoliquiritin (r=0.999 8), 0.155 8-1.558 μg for liquiritigenin (r=0.999 6), 0.187 1-1.871 μg for glycyrrhizic (r=0.996 9) and 0.158 1-1.581 μg for isoliquiritigenin (r=0.999 5), respectively. The average recoveries were 101.47% (RSD=1.272%), 101.09% (RSD=1.937%), 101.14% (RSD=2.388%), 100.38% (RSD=1.448%) and 100.86% (RSD=1.759%), respectively (n=5). CONCLUSION: This method has good resolution and high precision, and can be used for the quality control of Glycyrrhizae Radix et Rhizoma and Longchai decoction.
ABSTRACT
In this study, laser scanning confocal microscopy (LSCM) was used to determine the location and relative quantity of flavonoids in the leaves of Apocynum venetum L. from the top, middle and basal parts of the branch. The leaves of the plants of one, two and three years old, separately, were collected in July. ANOVA and LSD test were employed in the statistical analysis. The results indicated that flavonoids located mainly in xylem conduit of vein, collenchyma, epidermic cells and cuticle. The data of flavonoids contents under statistical analysis showed that difference existed in the leaves of different parts and different ages. This study provided the reliable scientific material about the analysis of the ecological and the exploitation of the leaves of Apocynum venetom L.