ABSTRACT
The purpose of this study is to elucidate the molecular mechanism of one-way serological reaction between XJ-160 virus and SINV by recombinant viruses which exchanged the glycoprotein genes individually or simultaneously. Three recombinant viruses were obtained based on the whole-length infectious cDNA clone of XJ-160 virus. The infectivity and pathogenesis to BHK-21 cells and animals were studied and the gene which controlled this one-way serological reaction phenomenon was searched by MCPENT. The results showed that the E2 glycoprotein was the main factor which influenced the growth rate, plaque morphology and pathogenicity of BHK-21 cells and suckling mice. The results of MCPENT showed that the E2 glycoprotein of SINV played a major role in this one-way serological reaction phenomenon. Our study identified the SINE2 gene was the determined gene for one way serological reaction between XJ-160 virus and SINV, and this research laid the foundation for further analysis of the genomic structure and function of SINV.
Subject(s)
Animals , Female , Mice , Alphavirus , Genetics , Allergy and Immunology , Physiology , Amino Acid Sequence , Cell Line , DNA, Recombinant , Genetics , Genetic Engineering , Glycoproteins , Chemistry , Metabolism , Mice, Inbred BALB C , Molecular Sequence Data , Neutralization Tests , Sindbis Virus , Allergy and Immunology , Viral Load , Viral Proteins , Chemistry , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.</p><p><b>METHODS</b>PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.</p><p><b>RESULTS</b>Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.</p><p><b>CONCLUSION</b>Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.</p>