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Objective To investigate the effect of sperm preparation in male infertility on sperm morphology and DNA fragmentation index(DFI). Methods Four hundred men were divided into the groups of fertile , teratozoospermics and unexplained subfertility. Sperm morphology and DFI were analyzed before and after the sperm preparation. Results Sperm abnormal morphology and DFI in the infertility group were higher than those in the fertile group ,but significantly decreased after sperm preparation. The method of density gradient separation results in obtaining better sperm(P<0.01). Conclusions Compared to the method of swimming up ,the method of density gradient separation could result in obtaining sperm with improved normal morphology and DNA integrity.
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<p><b>OBJECTIVE</b>To establish an accurate, fast and simple screening method for AZF microdeletions using capillary technology and use it for clinical testing.</p><p><b>METHODS</b>For each pair of primers, the 5' end of either forward or reverse primer was labeled with a FAM, JOE or TAMRA fluorescence dyes to establish multiplex quantitative fluorescence PCR systems for the establishment of a screening method of Y chromosome AZF microdeletions by capillary technology. The detection of Y chromosome AZF microdeletion was carried out on 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia.</p><p><b>RESULTS</b>A screening method for Y chromosome AZF microdeletions using capillary technology was established. Thirty eight cases of AZF microdeletions were found among 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia, which gave a deletion rate of 5.24%. Y chromosomal microdeletions were found in 8.62% of the azoospermia group, 6.75% of the oligozoospermic group, and 2.23% of the asthenospermia group.</p><p><b>CONCLUSION</b>An accurate, fast and simple screening method of Y chromosome AZF microdeletions by capillary technology has been established, which may have an important clinical value.</p>
Subject(s)
Adult , Humans , Male , Azoospermia , Genetics , Capillary Action , Chromosome Deletion , Chromosomes, Human, Y , Infertility, Male , Multiplex Polymerase Chain Reaction , Sex Chromosome Aberrations , Sex Chromosome Disorders of Sex Development , DiagnosisABSTRACT
Glucocorticoid receptor (GR) is identified as a member of nuclear receptor family. To exert its biological action, the ligand bound GR is translocated from the cytoplasm into the nucleus by regulating transcriptional signals of related genes. In clinical practice, the effects of glucocorticoid are often mediated by GR signaling pathways. An increasing number of studies have indicated that GR signaling pathways play an essential role in the proliferation, invasion and prognosis of bladder cancer. Meanwhile, the new-generation selective GR activator improves its anti-tumor effects, and at the same time reduces the adverse reactions of hormones, which probably raises the prospect for the treatment of bladder cancer.
Subject(s)
Animals , Humans , Antineoplastic Agents , Pharmacology , Cell Nucleus , Genetics , Prognosis , Protein Transport , Genetics , Receptors, Glucocorticoid , Physiology , Signal Transduction , Genetics , Transcriptional Activation , Physiology , Urinary Bladder Neoplasms , GeneticsABSTRACT
Objective To investigate mechanism of chronic bacterial prostatitis (CBP) causes increased oxidative stress and oxidative damage in the patients. Methods Seventy CBP patients (CBPPs) sampled randomly, and seventy healthy adult volunteers(HAVs) sampled randomly, were enrolled in a case-control study, in which the levels of nitric oxide (NO), vitamin C (VC), vitamin E (VE), and (-carotene ((-CAR) in plasma, as well as the level of malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in erythrocytes were determined by spectrophotometry. Results Compared with the average values of NO and MDA in the HAVs group((378?33)nmol/L和(28.6?4.4)nmol/g?Hb),the average values in the CBPPs group ((426?31)nmol/L and (34.7?4.7)nmol/g?Hb)were significantly increased, while VC, VE, ?-CAR, SOD, CAT, and GPX in the CBPPs group((47.4?11.6)?mol/L,(18.1?4.8)?mol/L,(1.4?0.4)?mol/L, (1 912?221)U/g?Hb, (254?67) K/g?Hb and (25.0?5.0) U/g?Hb) were significantly decreased compared with the average values in the HAVs group((55.1?13.4)?mol/L、(25.7?4.5)?mol/L、(1.7?0.5)?mol/L、(2 081?222)U/g?Hb、(294?77)K/g?Hb和(28.8?5.1)U/g?Hb)).The partial correlation analysis between the course of disease and the said each parameter for 70 CBPPs while controlling for the age suggested that with prolonged course of disease the values of NO and MDA were gradually increased (rNO=0.480 1,rMDA=0.436 4,P
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【Objective】To investigate the effect of Anti-virus Capsule(AVC) on quality of life(QOL) of patients with recurrent genital herpes(RGH).【Methods】The universal QOL measurement scale of WHOQOL-BREF was used to evaluate the QOL status of 40 RGH patients before and after treatment.The recurrence rate and skin lesion healing time were also observed before and after treatment.【Results】After treatment,QOL was much improved,mean recurrence rate was decreased and skin lesion healing time was shortened,the difference being significant(P
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Objective To investigate the correlation of clinical and pathological characteristics of bladder transitional cell carcinoma(BTCC) with platelet-derived endothelial cell growth factor (PD-ECGF). Methods Expression of PD-ECGF,VEGF and MVD were examined in the specimens of 52 cases of BTCC by Envision immunohistochemical staining.All the 52 cases were followed up via interview for a period of 5 years. Results Of the 52 cases 50 provided complete relevant data.The 5-year postoperative tumor-free survival rate was 42.9%(21/49) and overall survival rate was 78.0%(39/50).The positive expression rate of PD-ECGF in G 1 tumors was 17.6%(3/17);in G 2,59.3%(16/27) and in G 3,87.5%(7/8)(P