ABSTRACT
<p><b>OBJECTIVE</b>To examine serum levels of interleukin-13 (IL-13) and tumor necrosis factor alpha (TNF-alpha) in children with Mycoplasma pneumoniae (MP) pneumonia.</p><p><b>METHODS</b>Eighty children with MP pneumonia complicated by wheezing or without (n=40 each), 40 children with pneumonia from non-MP infection and 40 healthy children were enrolled. Serum levels of IL-13 and TNF-alpha were measured using ELISA.</p><p><b>RESULTS</b>The serum levels of IL-13 and TNF-alpha in the MP pneumonia group were significantly higher than those in non-MP pneumonia group and the healthy control group (P<0.01). The children with MP pneumonia complicated by wheezing had increased serum levels of IL-13 (214.6 + or - 67.2 ng/L vs 189.6 + or - 52.1 ng/L; P<0.01) and TNF-alpha(0.55 + or - 0.13 ng/mL vs 0.42 + or - 0.16 ng/mL; P<0.01)compared with those without wheezing.</p><p><b>CONCLUSIONS</b>The increase in serum levels of IL-13 and TNF-alpha may play important roles in the pathogenesis of MP pneumonia and wheezing attack in children.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Infant , Male , Interleukin-13 , Blood , Pneumonia, Bacterial , Allergy and Immunology , Pneumonia, Mycoplasma , Allergy and Immunology , Respiratory Sounds , Allergy and Immunology , Tumor Necrosis Factor-alpha , BloodABSTRACT
Objective To explore intervention effect of retinoic acid(RA) on hyperoxic lung fibrosis in neonatal rat,and to observe the role of transforming growth factor-?1(TGF-?1)and ?-smooth muscle actin(?-SMA)in hyperoxic lung fibrosis.Methods The SD neonatal rats were randomly divided into 4 groups:air-exposed control group(group Ⅰ),air-exposed and RA-treated group(group Ⅱ),hyperoxia-exposed control group(group Ⅲ),hyperoxia-exposed and RA-treated group(group Ⅳ).The rats of group Ⅲ,Ⅳ were kept in chambers containing 850 mL/L oxygen,the other 2 groups were exposed to air.The rats of group Ⅱ,Ⅳ were intraperitoneally injected with RA [500 ?g/(kg?d)],group Ⅰ,Ⅲ were intraperitoneally given the same dose of oleum lini.At the end of exposure,the lung histophatholoical changes and radical alveolar counts(RAC) were observed by HE staining under light microscope,the degree of pulmonary fibrosis was evaluated by Masson trichrome method and fibrosis score.The protein expression of TGF-?1 and ?-SMA were determined by immunostaining.Results At 14 d of exposure,group Ⅲ resulted in a significant increase in fibrosis score and expressions of TGF-?1 and ?-SMA compared with group Ⅰ,Ⅱ and Ⅳ(Pa0.05).Conclusion TGF-?1 and ?-SMA may have important role in hyperoxic induced lung fiborsis injury,the earlier period intervention of RA can reduce lung fibrosis regeneration and exceptional alveolus development.
ABSTRACT
Objective To explore the protective effect of Ligustrazine on pulmonary fibrosis caused by hyperoxia in neonatal rats.Met-hods Eighty Sprague-Dawley(SD)clean neonatal rats,which were less than 12 hours old,were enrolled in this study.The neonatal rats were randomly divided into 4 groups:air-exposed control group(group A);air-exposed plus Ligustrazine-treated group(group B);hype-roxia-exposed control group(group C);hyperoxia-exposed plus Ligustrazine-treated group(group D).The neonatal rats of group B and group D were intraperitoneally injected with Ligustrazine[30 mg/(kg?d)] in solution of normal saline(NS),group A and group C were intraperitoneally given the same dose of NS.On 14th day,10 rats of each group were randomly chosen and then killed,the pulmonary pathological changes were observed by HE staining under light microscope.The area percentages of collagen fibers were measured by Masson staining and imaging analysis.The degree of pulmonary fibrosis was evaluated by the area percentages of collagen fibers and hydroxyproline content.The results were analyzed by SPSS 13.0 software.Results On the 14th day of the exposure,the histopathologic examination demonstrated broadened lung interstitium and reduced alveolar quantity and lung fibrosis were developed in group C;Ligustrazine treatment obviously alle-viated the changes of lung histomorphology.The newborn rats in group C had lower body weight compared that of with group A,B(Pa0.05).Conclusion Early use of Ligustrazine can decrease the severity of the pulmonary fibrosis of neonatal rats exposed to hyperoxia.