Value of early diagnosis and prognosis assessment of serum sCD 40L and NT‐proBNP levels detection in aged patients with heart failure / 心血管康复医学杂志

Objective :To study application value of serum sCD40L and NT‐proBNP levels detection in early diagnosis and prognosis assessment of aged patients with heart failure (HF).Methods :A total of 73 aged HF patients treated in our hospital from Feb 2015 to Feb 2017 were selected as HF group .According to NYHA cardiac function class , HF group was divided into class Ⅱ group (n＝37) and class Ⅲ group (n＝36).A total of 112 healthy people ,who had a physical examination in our hospital at the corresponding period ,were selected as health group .HF group were treated for one month .Incidence of major adverse cardiovascular events (MACE) within one‐month was re‐corded .According to MACE during one‐month follow‐up ,HF group was divided into MACE group (n＝20) and no MACE group (n＝53).Serum levels of sCD40L and NT‐proBNP were measured and compared between HF group before treatment and health group ;class Ⅱ group and class Ⅲ group before and after treatment ;MACE group and no MACE group .Results :There were significant rise in serum levels of sCD 40L and NT‐proBNP in HF group be‐fore treatment compared with health group , P＝0. 001 all.Compared with before treatment ,there were significant reductions in serum levels of sCD40L and NT‐proBNP in class Ⅱ group and class Ⅲ group , P＝ 0.001 all.After treatment ,compared with class Ⅲ group there was significant reduction in serum level of sCD40L [ (8.31 ± 0.76) ng／ml vs .(7.37 ± 0.81) ng／ml] in class Ⅱ group , P＝0. 001. During one‐month follow‐up ,20 cases of MACE oc‐curred in HF group (n＝73) and incidence of MACE in HF group was 27.40% .Compared with MACE group ,there were significant reductions in serum levels of sCD 40L [ (10.26 ± 1.54) ng／ml vs.(11. 49 ± 1. 81) ng／ml] and NT‐proBNP [(612. 28 ± 122. 76) ng／L vs.(1072.25 ± 245.68) ng／L] in no MACE group ,P＝0. 001 all.Conclusion :Se‐rum sCD40L and NT‐proBNP levels detection possesses good application value in early diagnosis and prognosis assess‐ment for aged patients with HF ,which is worth extending .

Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells / 中国结合医学杂志

<p><b>OBJECTIVE</b>To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells.</p><p><b>METHODS</b>The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions.</p><p><b>RESULTS</b>Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P <0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P <0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P <0.05).</p><p><b>CONCLUSION</b>Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.</p>

Molecular mechanisms involved in regulation of proliferation and apoptosis by STAT3 antisense oligonucleotide and chemotherapy in laryngeal cancer cells / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To study the mechanism of STAT3 antisense oligonucleotide (STAT3 AS-ON) in combination with DDP in the treatment of laryngeal cancer.</p><p><b>METHODS</b>STAT3 AS-ON, DDP, or STAT3 AS-ON + DDP was added into culture media. The expression and phosphorylation levels of STAT3 protein in Hep-2 cells were measured by Western Blot. The expression of Cyclin D1 and Bcl-xL was also detected by Western Blot. The cell proliferation was assayed by methyl thiazolyl tetrazolium (MTT). Flow cytometry was performed to analyze the cell cycle and apoptosis.</p><p><b>RESULTS</b>STAT3 was highly expressed and phosphorylated in Hep-2 cells. Transfection of STAT3 AS-ON suppressed the expression and phosphorylation levels of STAT3 protein. Forty-eight hours after transfection, the proliferation of Hep-2 cells was inhibited in a dose-dependent manner. Inhibitory effects appeared at 24 h after transfection of STAT3 AS-ON, and became more obvious after 36 h. Seventy-two hours after transfection, the rate of apoptosis in STAT3 AS-ON + DDP group, DDP group, STAT3 AS-ON group, STAT3 S-ON group, lipidosome group and control group was 32.9%, 13.5%, 28.1%, 3.2%, 2.4%, 1.8% respectively. After the treatment of Hep-2 cells with STAT3 AS-ON and DDP for 72 h, the ratio of G1 phase was up-regulated from 55.7% to 74.9%, while that of S phase was own-regulate from 33.6% to 6.9%.</p><p><b>CONCLUSIONS</b>STAT3 AS-ON and DDP could suppress the growth of laryngeal cancer cells and induce significant apoptosis of laryngeal cancer cells. Combined use of them had a synergic effect, obviously inhibiting the activation of STAT3 signaling transduction pathway of laryngeal cancer cells. Selective inhibition of specific signaling pathway may provide a new therapeutic approach for treating laryngeal cancers.</p>

The inhibitive effect of low frequency electric brain stimulation on amygdale kindling in rats and its possible mechanisms / 中华物理医学与康复杂志

Objective To investigate the effect of low frequency electric deep brain stimulation on amygdale kindling in rats.Methods The amygdale kinkling model of rats was established by operation on the brain.The effects of low frequency deep brain electric stimulation used alone or in combination with anti-epilepsy drugs were ob- served in terms of severity of seizure attack reflected by Racine's scale and afterdischarge duration recorded in electro- encephalogram.Results Fifteen minutes of low frequency electric stimulation at 1 Hz and 100 to 350?A effective- ly inhibited amygdale kindling as demonstrated by a significant decrease of afterdischarge duration,and decreased the severity of seizure attack (P

Effect of verapamil on electrobiological activity and seizure behavior in phenytoin-carbamazepine resistant rats / 中华神经科杂志

Objective To invesgate the effect of P-glycoprotein(PGP)inhibitor,verapamil,on electrobiological activity and seizure behavior in phenytoin-carbamazepine(PHT-CBZ)resistant rats.Methods The model of medically intractable epilepsy was established by kindling of amygdale. Verapamil was applied to PHT-CBZ resistant rats,followed by the observation on after discharge threshold (ADT),after discharge duration(ADD)and seizure activity.Results Compared with the control group, the ADT was higher in PHT-CBZ resistant rats peritoneally injected with verapamil((238.0?32.2)?A vs (177.0?23.3)?A,P