Cytotoxicity of Paecilomyces tenuipes Against Human Carcinoma Cells, HepG2 and MCF-7 In Vitro

The methanolic extract of fruiting body of Paecilomyces tenuipes DGUM 32001 showed significant cytotoxicity against human cancer cells: HepG2 and MCF-7. The methanolic extract was further fractionated with organic solvents such as chloroform and ethyl acetate in that order. Among the fractions tested, the ethyl acetate fraction showed the highest cytotoxicity against the carcinoma tested. The IC50 values of ethyl acetate fraction against HepG and MCF-7 were 40 and 9.6 microg/ml, respectively.

Short-term Change of Corneal Endothelium after Phacoemulsificatin using Phaco-drill Technique

Phaco-drill technuque, is a method of phacoemulsifying cataractous lens with a bevel down position of phacotip, thus concentrating vacuum and ultrasonic power on center of the lens. This enables the phacoemulsification to be completed with a lower setting of energy. In this study, we evaluated the efficacy of the phaco-drill technique by measuring corneal endothelial cell damage. Since August 1997, we examined 170 patients who had under-gone phacoemulsification using phaco-drill technique, for uncorrected visual acuity, number, shape and size of corneal endothelial cell, and corneal thickness before and 2 months after surgery. the mean visual acuity was 0.26+/-0.24 before operation,and the mean visual acuity at 1 day and 2 months postlperatively was 0.59+/-0.32 and 0.82+/-0.26, respectively. The mean time of phacoemulsification was 58+/-41 seconds, and the average power(surgeon mode)was 8.6+/-0.26%. Mean volume of irrigating solution used in the operation was 116.12+/-52.10cc and that of aspirated solution was 63.34+/-30.37cc. The number of endothelial cells decreased from 2450+/-308.6 cell/mm2 before operation to 2234+/-372.2 cell/mm2 2 months after operation(9.2% decrease). The size of endothelial cells increased from 33.78+/-5.53/micrometer(2) before operation to 34.70+/-5.79/micrometer(2) 2 months after the operation(2.7% increase). The regularity of the cells (hexagonality) showed a decrease of 3%, from 57.47+/-10.16 before operation to 55.81+/-10.49% 2 months after the operation. The corneal thickness increased from 528+/- 36micrometer before operation to 590+/-8micrometer 1 dat(11.7% increase) and 531+/-4micrometer(0.5% increase) after the operation. From the results of this study we suggested that phaco-drill technique might be one of effective methods of phacoemulsification for less damage of corneal endothelial cells and less fluid consumption.

Clinical Results of AMO ARRAY Multifocal Intraocular Lens

Various kinds of multifocal intraocular lenses(IOLs) have been developed for the improvement of near and far visual acuity after cataract operation. This prospective study was performed to compare a function of AMO ARRAY multifocal IOL(Array group) with conventional monofocal IOL(Mono group). Each type of IOL was implanted into 25 eyes and uncorrected visual acuity(UCVA) and best corrected visual acuity(BCVA) at near, intermediate and far distance were evaluated before and 2 months after operation in both groups. Contrast sensitivity, photophobia and glare were also evaluated as subjective symptoms in both groups. Mean UCVA at distant and near distance before operation was 0.23+/-0.15 and 0.25+/-0.20 in mono group, and 0.13+/-0.11 and 0.14+/-0.11 in Array group. Mean UCVA/BCVA at distant and near distance 2 month after operation was improved to 0.79+/-0.28/0.87+/-0.27 and 0.34+/-0.15/0.82+/-0.26 in mono group and 0.83+/-0.19/0.86+/-0.19 and 0.50+/-0.15/0.79+/-0.15 in Array group. The difference of UCVA at near distance was statistically significant(P<0.05), while that of BCVA at near and far distance was not statistically significant between two groups(P<0.05). Array group had less contrast sensitivity at all spatial frequencies(1.5, 3, 6, 12, 18 cycles/degree) and more glare than the mono group after operation. AMO ARRAY multifocal IOL induces a good result of visual rehabilitation in both near and far distance, but it may cause more glare and less contrast sensitivity than the monofocal IOL.

A Case of Ocular Rosacea Treated with Oral Tetracycline

Rlsacea is chronic disease of the eye and facial skin, which is common in Caucasian. Ocular rosacea is often difficult for ophthalmologists to diagnose, especially when skin manifestations are not evident yet. Early diagnosis and early treatment is important to prevent its serious complications including corneal opacity and corneal perforation. A case of ocular rosacea in a 52-year-old Korean lady is reported. The patient was presented with hyperemic conjunctiva and vascularization, infiltration and thinning of the peripheral cornea. She did not have any specific skin lesion. But 20 years ago, she had suffered from rosacea. After administation of oral tetracycline and topicale and topical corticosteroid, the signs and symptoms of the ocular rosacea was markedly improved.

A Case of Ocular Rosacea Treated with Oral Tetracycline

Rlsacea is chronic disease of the eye and facial skin, which is common in Caucasian. Ocular rosacea is often difficult for ophthalmologists to diagnose, especially when skin manifestations are not evident yet. Early diagnosis and early treatment is important to prevent its serious complications including corneal opacity and corneal perforation. A case of ocular rosacea in a 52-year-old Korean lady is reported. The patient was presented with hyperemic conjunctiva and vascularization, infiltration and thinning of the peripheral cornea. She did not have any specific skin lesion. But 20 years ago, she had suffered from rosacea. After administation of oral tetracycline and topicale and topical corticosteroid, the signs and symptoms of the ocular rosacea was markedly improved.