ABSTRACT
Objective:To observe effect of ginsenoside Rh2 (GRh2) on the invasion and migration of colon cancer resistant cells HCT116/L-OHP and its specific mechanism. Method:Cell counting kit-8 (CCK-8) assay was used to detect the inhibitory effect of different concentrations of GRh2 (0, 2.5, 5, 10, 20, 40 mg·L-1) on HCT116/L-OHP cell proliferation, scratch assay, Transwell assay and adhesion assay were used to detect the effects of GRh2 (0, 2.5, 5, 10 mg·L-1) on cell migration, invasion and adhesion. The protein expression levels of E-cadherin and matrix metalloproteinase-9(MMP-9) were examined by Western blot. Result:Compared with control group, GRh2(5, 10, 20, 40 mg·L-1) significantly inhibited the proliferation of HCT116/L-OHP cells in a dose-dependent manner(PP2 group (5, 10 mg·L-1) was significantly decreased (PP2 group was significantly decreased (PP2 group was significantly reduced (PP2 (10, 20, 30 mg·L-1) promoted E-cadherin protein expression (PPPConclusion:GRh2 can significantly inhibit the invasion and migration of HCT116/L-OHP in colon cancer cells, and its potential mechanism may be related to the promotion of E-cadherin and the inhibition of MMP-9 expression in a dose-dependent manner.
ABSTRACT
With the deepening of research on intestinal microbiota, more and more evidence indicates that intestinal microbiota affects various physiological processes of the human body and is closely related to tumors, Researches show that intestinal microbiota can promote tumorigenesis, enhance the antitumor effect of chemotherapeutic drugs, mediate chemoresistance, regulate the effect of immunotherapy, and reduce intestinal damage during radiotherapy. The phenomena and mechanisms revealed in the above studies are incredible. Based on the above correlation, the genus found in the study are expected to become the new target of tumor prevention and treatment. The evidence obtained from current animal and clinical trials is refreshing, however, in view of the complexity of human intestinal microecology and the limitations of human understanding, its potential role in tumorigenesis and treatment still needs systematic and in-depth research.
ABSTRACT
<p><b>OBJECTIVE</b>By means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.</p><p><b>METHODS</b>Two human gastric cancer cell lines were used: GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.</p><p><b>RESULTS</b>After three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides: TLNINRLILPRT and SMSI(X)SPYI(XXX).</p><p><b>CONCLUSION</b>Two peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.</p>