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Objective To observe the changes of electrical property of the 12 Source-points in encephaloma patients undergoing surgery.Methods A total of 116 encephaloma patients and 60 healthy people who signed the informed consent were enlisted in the present study.The regional cutaneous electric resistance(CER) of the bilateral 12Yuan(Source)-points was measured in the afternoon(14:00-16:00) before and one week after surgery under room temperature [(22?3)℃,(55?10)% in humidity] by using "Meridian Energy Analysis Device".Results In comparison with normal subjects,CER values of the 12 Source-points on both sides of the body in encephaloma patients were significantly lower(P
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Objective To investigate the effects of prednisone(PRD) on experimental allergic encephalomyelitis(EAE) and the mechanism.Methods Lewis rats were injected with mixture of guinea pig spinal extracts and Freund's adjuvant(FA) to induce EAE.PRD(5 mg/kg) was orally given ten days after the injection.The clinical manifestation of rats,their peripheral blood lymphocytes CD3~(+),CD4~(+),CD8~(+) and NK subsets,and pathology changes of brain and spinal were studied on day 26.Results In model CD3~(+) ratio is 63.2%,CD4~(+)ratio 46.8%,CD8~(+)ratio 17.4% and NK ratio 10.2%.In PRD CD3~(+) ratio is 69.5%,CD4~(+)ratio 51.8%,CD8~(+)ratio 18.1% and NK ratio 5.3%.PRD obviously decreased the clinical scores of EAE rats and peripheral blood NK ratio,increased CD3~(+) and CD4~(+)ratio,inhibited brain inflammatory reaction and demyelination.However,PRD didn't affect peripheral blood lymphocytes CD8~(+) and CD4~(+)/CD8~(+)ratio.Conclusions PRD is effective in the treatment of EAE rats,and the therapeutic mechanism may be contributed to the decrease of the peripheral blood NK ratio and increase CD3~(+) and CD4~(+)ratio.
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Objective To observe the immunohistochemical expression of lymphocyte subpopulationin in brain and spinal cord tissue,and explore the mechanism of Zuogui wan and Yougui wan on rats with experimental autoimmune encephalomyelitis(EAE).Methods Lewis rats were immunized with the myelin basic protein(MBP).120 rats were grouping randomly into normal group,EAE group,prednisone group,Zuoguiwan group and Youguiwan group after post immunization(PI).Rats in normal group and EAE group were administered normal soline,each 3 mL/d.Rats in prednisone group were administered suspension of prednisone after developed clinical signs,each 5 mg/kg.Rats in Zuoguiwan group were administered suspension of Zuoguiwan,each 2 g/kg,and rats in Youguiwan group were administered suspension of Youguiwan,each 3 g/kg.On 15th and 27th day after PI,rats were killed and the immunohistochemical staining was performed on the sections of brain and spinal cord.Results On 15th and 27th day after PI,the expression of CD4+ in brain and spinal cord tissue of EAE group were significantly higher than that in normal group(P
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0.05) in sex,attack frequency,cause of disease,MRI and EEG among 202 patients. The TCM syndrome of weak in spleen and phlegm excess was the main type of child patients,while deficiency in liver and kidney was common in middle-aged and old patients,and they had prominent statistical significance (P 0.05) in sex,attack frequency,cause of disease,MRI and EEG. The weak in spleen and phlegm excess type is common in children,and the deficiency in liver and kidney type is usual in middle-aged and the elderly. Long disease course manifests deficiency or deficiency mixed with excess syndromes,while short course mainly shows excess syndromes. Earlier age of onset manifests deficiency or deficiency mixed with excess syndromes,and onset in youth and middle age often show excess syndromes. Discharge location in temporal lobe is often of wind phlegm type,while discharge location in frontal lobe often belongs to phlegm fire disturbing upper body type. Weak in spleen and phlegm excess type often can be seen in general discharge.
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Insomnia is a commen disease which disturbs life of modern persons terribly.As usual,insomnia means the subjective experience the short time of sleep and poor sleep quality which will influence the normal activity in the day-time.Here are the manifestations of insomnia:tired,dizziness,headache,tinnitus,palpitation,not concentration,poor efficiency etc.In this article,through the study on traditional twelve two-hour thery,the author connects the theory to the real reasons of insomnia and finds out the suitable time for patients to take the medicine to get twice the power with half the effort.
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Objective: To investigate into the etiological factor,distribution of syndrome(patterns),pathogenesis features as well as the curative effects of small compound prescriptions of(Traditional) Chinese Medicine(TCM) in the treatment of acute stroke in stroke unit.Methods:(Cases) corresponding to diagnosis standards were randomly divided into control group(102 cases) and treatment group(156 cases).Conventional treatment methods were given to cases in control group.On the base of control group,small compound prescriptions were added to in treatment group,successively 14-21 days.The relation between types and time and TCM scale(TCMS),the NIH stroke scale(NIHSS) and Barthel index(BI) were observed in the two groups.Results: Stroke was age-related,the older,the more stroke patients.Stroke was closely related to hypertension.Ascending hyperactivity of liver yang was main type in the previous three days,this type(decreased) with the time going on.On the contrary,the type of wind phlegm and blood stasis was secondly main type in the previous three days,it increased with the time going on.Types of ascending hyperactivity of liver yang,wind phlegm and blood stasis as well as phlegm heat and constipation covered 95 per cent of all patients in the previous 21days.In the two groups,the mean scale of TCM and NIH decreased,and BI increased after treatment,this statistical difference could also be seen between the two groups.Conclusion: Excessive types are main types in the acute stage of stroke.Types of ascending hyperactivity of liver yang,wind phlegm and blood stasis as well as phlegm heat and constipation are its main types.Transferring of ascending hyperactivity of liver yang into endogenous wind may be trigger of reaction link of wind,fire,phlegm and blood stasis in TCM theory.Small compound prescriptions of TCM aiming to clearing away liver fire to stop wind,to activate blood for removing blood stasis and phlegm,to removing phlegm and heat from discharge can reduce scale of TCM and NIH,increase BI in the treatment of patients in stroke unit,which provide beneficial proofs for evaluation of effects of TCM.
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0.05).Conclusion:The combination of TCM with western medicine was superior to simple western medicine in treating multiple sclerosis.Regulation of cytokines(TNF-? and IL-12 p40) in plasm may be partial reasons for the clinical effects of treatment based on syndrome differentiation.
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On basis of syndrome differentiation of TCM, 1 case of brain stem glioma was treated with decoction and Xihuangwan produced by Tongrentang. She has lived past 3 years with good life quality. More attention should be paid to the function of liver, spleen and stomach. The method of checking liver-yang and smoothing liver-qi, strengthening spleen-qi and harmonizing stomach-qi were used. Smoothing liver-qi can keep good blood and liquid circulation, and can reduce phlegm and blood stasis in the body; Checking liver-yang can prevent brain being disturbed by internal wind. Strengthening spleen-qi and harmonizing stomach-qi was the source of qi-blood. Xihuangwan can directly arrive at brain tumor because of its specific fragrant, it can smooth the qi and remove the blood stasis, can clear heat and detoxify. Only with the combination of disease and syndrome differentiation, whole body and local tumor, dispelling xie and strengthening zheng, can this case be treated successfully.
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Objective: To observe the clinical effect of Huoxue Tongqiao Decoction on nervous tinnitus. Methods: 60 Patients(90 ears) were randomly divided into treatment group(30 cases, 44 ears) and control group(30 cases, 46 ears). Patients of control group took orally sibelium and oryzanol. Based on the control group, patients of treatment group took Huoxue Tongqiao Decoction to treat tinnitus. Results: After treatment, the total effi cient of treatment group was 86.4%, while that of control group was 63.0%. There was signifi cant difference(?2=13.83,P
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Objective: To observe the immunohistochemical expression of interferon-? (IFN-?), matrix metalloproteinase -9(MMP-9) in brain and spinal cord tissue, and explore the action mechanism of Zuogui Pills and Yougui Pills on rats with experimental autoimmune encephalomyelitis(EAE). Methods: Lewis rats were immunized with the myelin basic protein (MBP).120 Rats were grouped randomly into nomal group, EAE group, prednisone group, Zuogui Pills group and Yougui Pills group after post immunization (PI). Rats in normal group and EAE group were administered sodium, each 3ml/d, rats in prednisone-group were administered suspension of prednisone after developed clinical signs, each 5mg/kg, rats in Zuogui Pills group were administered suspension of Zuogui Pills, each 2g/kg, and rats in Yougui Pills group were administered suspension of Yougui Pills each 3g/kg. On days 15 and 27 PI, rats were killed and the immunohistochemical staining was performed on the sections of brain and spinal cord. Results: On days 15 and 27 PI, the expression of IFN-? and MMP-9 in central nervous system of EAE group were higher than those in nomal group (P
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Objective: To observe the effect of ZuoGuiWan and YouGuiWan on induced experimental autoimmune encephalomyelitis(EAE) in Lewis rats,compare the therapeutic effect between ZuoGuiWan and YouGuiWan,and explore the underlying immunoregulatory mechanism between warming-yang and nourishing-yin therapy.Methods : 120 male Lewis rats were bred at Capital Medical University under specifi c pathogen-free conditions.24 rats were selected as normal group before immunization.The other rats were randomly devided into 4 groups after immunization,including EAE group,prednisone group,ZuoGuiWan group and YouGuiWan group.Rats of normal group were injected with normal sodium in the hind feet pad on both sides,the other rats were injected with the antigen consisted of myelin basic protein(MBP) and complete Freund’s adjuvant(CFA) to induce EAE.Rats of normal group and EAE group were administered with normal sodium after immunization,rats of prednisone group with prednisone(5mg/kg),rats of ZuoGuiWan group with ZuoGuiWan(2g/kg),rats of YouGuiWan group with YouGuiWan(3g/kg).On the day 15 and 27 after immunization,the peripheral blood was collected,lymphocyte subgroups and NK cells were detected by ? ow cytometer.Results: On day 15 after immunization: In peripheral blood,the number of CD4+lymphocyte in ZuoGuiWan group was signifi cantly higher(P
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Kidney deficiency is the core of the currence of multiple sclerosis.Invigorating the kidney is the core in treatment of multiple sclerosis,though there are some other commonly used methods,such as dissipating phlegm,promoting blood flow,clearing heat,dispelling dampness and diuresis in aspects of eliminating pathogen,and invigorating spleen for benefiting qi,dispersing stagnated liver-qi,calming mind for tranquillizing in aspects of regulating the function of organs.Either invigorating the kidney is used through the whole course of disease or used at the later stage,it plays an important role in treatment of multiple sclerosis.
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Disseminated sclerosis(DS) is an autoimmune demyelinating disease of the central nervous system(CNS) that attacks generally the youth.The common symptoms of DS are hypopsia,paralysis,abnormal sense,urine deposited,dysfunction of bowels,etc.Sometimes MS also injures neural advanced functions.Traditional Chinese Medicine(TCM) mainly defines DS as "atrophy syndrome" according to its clinic symptoms.During acute relapsing period,most DS patients present deficiency of yin of both the kidney and liver,occurring with blood stasis and phlegm.Moreover,high dose methylprednisolone can activate pathogenic fire to damage the yin that aggravates deficiency of yin.Along with repeated relapse and evolvement of DS,the body's condition will change into syndrome of deficiency of both the yin and yang.So,the drugs of nourishing both the kidney and liver yin,activating blood circulation and clearing phlegm should be applied first to treat DS. Besides,the drugs of clearing heat,activating yang,motivating flow of qi,dredging the Fu-organ,invigorating blood and regulating liver-qi should also be used according to the different syndromes.
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AIM: To explore the mechanism of Nao-re-qing oral liquid (NRQ) decreasing endotoxin (ET)-induced fever in rabbits. METHODS: (1) The ET-induced fever model was established in rabbits. Febrile response of rabbits was observed. (2) The arginine vasopressin (AVP) content in the ventral septal area (VSA),and cAMP content in hypothalarmus (HP) and CSF were determined by radioimmunoassay.RESULTS: (1) In ET group,the maximal increment in body temperature (?T) [(1.80?0.16) ℃],6 h thermal respone index (TRI_6)(11.31?0.20),the cAMP content in the HP [(1.35?0.21)nmol/g],the cAMP content in CSF [(66.69?1.82) nmol/L] and AVP content in the VSA [(30.80?9.59)ng/g ] were significantly higher than those in NRQ+ET group[?T(0.82?0.08) ℃,TRI_6(5.73?0.09),HP: cAMP(0.70?0.50)nmol/g,CSF: cAMP(56.86?1.34),AVP:(11.91?3.47)ng/g]( P
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AIM:To observe the antipyretic effect of Naoreqing(NRQ)oral solution in rabbits with endotoxin-induced fever.METHODS:The ET-induced fever model was established in rabbits.The digital thermometer was used to register rectal temperature.The endothelin content in blood plasma,and tumor necrosis factor-?(TNF?)in blood serum were determined by radioimmunoassay.The activities of tissue-type plasminogen activator(t-PA)and plasminogen activator inhibitor(PAI)in plasma were measured by the assay of chromogenic substrate.At the same time,the pathology of lung,kidney and liver were observed.RESULTS:① NRQ showed obvious antipyretic effect in the rabbits with endotoxin-induced fever(P
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AIM: To investigate the antipyretic mechanism of Naoreqing (NRQ) oral liquid, a Chinese medicine. METHODS: ① Fever models of rabbits were established by intravenous. injection of endogenous pyrogen (EP). ② The antipyretic action of NRQ were observed. ③ cAMP contents in the hypothalamus (HP) and the cerebrospinal fluid (CSF) were determined by radioimmunoassay. RESULTS: ① NRQ obviously reduced body temperature of febrile rabbits induced by EP (P