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[Objective] To systematically study the characteristics of the key syndromes of systemic lupus erythematosus(SLE),summarize its clinical efficacy,and explore the mechanism of traditional Chinese medicine(TCM).[Methods] The main clinical syndrome features of SLE are heat-toxicity,blood stasis and Yin-deficiency.Compared with glucocorticoids(GCs) alone,combination with JP can significantly improve the clinical efficacy and reduce the glucocorticoid treat consumption effectively.[Results]Our results showed that in MRL/LPR model mice,JP could restore the balance between TH17 and regulatory T cells through CaMK4 signaling pathway,and the interaction between T cells and B cells.JP could promote DNA methylation,inhibit the over-activated lymphocytes,improve the prognosis of SLE and prevent renal damage.JP could up-regulate the concentration of eicosapentaenoic acid (EPA) and the level of glycine,phenylalanine and tryptophan dramatically.Furthermore,JP could reduce the abundance of pathogenic bacteria(such as Parabacteroides) and increase the abundance of beneficial bacteria (such as Odoribacter and Ruminococcus) by regulating gastrointestinal function,which was not found in GCs treated subjects.JP can also obviously reduce the expression of TLR7,TLR9 and IL-6 in intestinal mucosa,showing the effect of multi-target therapy on SLE.[Conclusion] The most common clinical syndromes of SLE are heat-toxicity,blood stasis and Yin-deficiency.JP can effectively control the progress of SLE and exert its clinical function from different aspects.Our research laid the scientific basis for the popularization and application of TCM in clinical treatment of SLE.
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Objective To investigate the relatio nship between the DNA methylation status of gluco-corticoid receptor (GR) gene promoter and mRNA expression level of GRα gene of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE). Methods Fifteen new onset SLE patients and fifteen healthy controls were enrolled in this study. The DNA methylation status of GR gene promoter 1 of PBMCs was detected through bisulfite sequencing polymerase chain reaction (PCR). The mRNA expression of GRα, DNA methyltransferases, growth arrest and DNA damage-induced 45α (GADD45α) of PBMCs was detected using the quantitative real-time polymerase chain reaction method. T-test and χ2-test were used to detect the differences between the two groups, Pearson's correlation coefficient was used to analyze the linear correlation between two variables. Results Compared with healthy controls, the mRNA expression of GRα was signi-ficantly declined in SLE patients (10±5, 17±7, t=2.69, P<0.05), and the mRNA expression of DNMT1 and GADD45α was significantly elevated in SLE patients (t=3.11, P<0.05 and t=2.98, P<0.05). The overall mean methylation status of the 142 CpG islands of the four promoters was significantly elevated in SLE patients [(16±8)%vs (11±6)%, t=2.75, P<0.05]. The global methylation status of PBMCs in SLE patients was obviously lower than healthy controls (t=4.39, P<0.05). Conclusion Hypermethylation of GRα promoter may result in GRαgene low expression in PBMCs of patients with SLE.
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[Objective]To establish a rat model of connective tissue disease associated interstitial pneumonia induced by bleomycin, and explore the effect of Qingfei Decoction on interstitial pneumonia and its effect on antifibrosis. [Methods]48 male SD rats , weight 180~200g. All the rats were fed adaptivelly for one week and the rat model of interstitial lung disease were established by means of pour 5mg·kg-1 BLM into trachea .Divided these rats into 4 groups:the normal control group, the interstitial pneumonia model group ,the predmisone group and the Qingfei Decoction group. In addition, each group 12 rats. After 28 days'medication, compare the pathological difference between Qingfei Decoction group with the interstitial pneumonia model group, the predmisone group and the normal control group, and the difference in the expressions of TNF-α, IL-6, TGF-β1 in lung tissue, and the expression of hydroxyproline in lung tissue. [Result]①Pathological staining showed that:compared with the normal control group, the destruction of lung tissue structure of rats pneumonia, severe inflammatory cell infiltration, fibrous tissue hyperplasia massive consolidation, alveolar distribution of collagen fiber lesions in model groups; compared with interstitial pneumonia model group, Qingfei Decoction group reduced lung tissue damage, reduce inflammation cell infiltration, fibrous tissue hyperplasia reduced collagen relative area decreased compared with model group. ②Compared with normal control group, the lung tissue of HYP rats was significantly increased, and the difference was statistically significant the model group( P<0.01); Compared with interstitial pneumonia in the model group, the content of HYP in lung tissue of rats Qingfei Decoction group decreased significantly, and the difference was statistically significant(P<0.01). ③Compared with normal control group, the model group of rat lung tissue IL-6, TNF- alpha, TGF- beta1 concentration increased significantly(P<0.05, P<0.01);compared with the model group, interstitial pneumonia, Qingfeifang lung tissue homogenate TNF-alpha, TGF-beta 1, IL-6 concentration decreased significantly(P<0.05, P<0.01). [Conclusion]1.Qingfei Decoction has inhibition effect to the bleomycin induced interstitial pneumonia.2.The treatment mechanism of Qingfei Decoction to BLM-ILD maybe related to TNF-α, IL-6, TGF-β1.
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[Objective]To establish a rat model of connective tissue disease associated interstitial pneumonia induced by bleomycin, and explore the effect of Qingfei Decoction on interstitial pneumonia and its effect on antifibrosis. [Methods]48 male SD rats , weight 180~200g. All the rats were fed adaptivelly for one week and the rat model of interstitial lung disease were established by means of pour 5mg·kg-1 BLM into trachea .Divided these rats into 4 groups:the normal control group, the interstitial pneumonia model group ,the predmisone group and the Qingfei Decoction group. In addition, each group 12 rats. After 28 days'medication, compare the pathological difference between Qingfei Decoction group with the interstitial pneumonia model group, the predmisone group and the normal control group, and the difference in the expressions of TNF-α, IL-6, TGF-β1 in lung tissue, and the expression of hydroxyproline in lung tissue. [Result]①Pathological staining showed that:compared with the normal control group, the destruction of lung tissue structure of rats pneumonia, severe inflammatory cell infiltration, fibrous tissue hyperplasia massive consolidation, alveolar distribution of collagen fiber lesions in model groups; compared with interstitial pneumonia model group, Qingfei Decoction group reduced lung tissue damage, reduce inflammation cell infiltration, fibrous tissue hyperplasia reduced collagen relative area decreased compared with model group. ②Compared with normal control group, the lung tissue of HYP rats was significantly increased, and the difference was statistically significant the model group( P<0.01); Compared with interstitial pneumonia in the model group, the content of HYP in lung tissue of rats Qingfei Decoction group decreased significantly, and the difference was statistically significant(P<0.01). ③Compared with normal control group, the model group of rat lung tissue IL-6, TNF- alpha, TGF- beta1 concentration increased significantly(P<0.05, P<0.01);compared with the model group, interstitial pneumonia, Qingfeifang lung tissue homogenate TNF-alpha, TGF-beta 1, IL-6 concentration decreased significantly(P<0.05, P<0.01). [Conclusion]1.Qingfei Decoction has inhibition effect to the bleomycin induced interstitial pneumonia.2.The treatment mechanism of Qingfei Decoction to BLM-ILD maybe related to TNF-α, IL-6, TGF-β1.
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[Objective]To explore LIU Wansu's methods in treating fire and heat disease from depression of yang qi.[Methods]To analyse LIU Wansu's understanding of fire and heat disease and highlight its treatment from depression of yang qi through reading his works like Exploration to Pathogenesis in Plain Questions,Yellow Emperor's Plain Questions on Xuanminglun Formulae and so on.[Results]LIU Wansu had profound medical knowledge,rich clinical experience and initiative spirit.Under the guidance of nineteen items of pathogenesis from Huangdi's Canon of Medicine,he expanded fire and heat disease and put up six exogenous pathogenic factors can transfer to fire theory.To be specific,excessive wind,cold,dampness and dryness would block Xuan Fu and depress yang qi,causing fire and heat disease.He regardeddepression of yang qi as a key link in six exogenous pathogenic factors' transferring to fire and put up cooling combined with ventilating method to dissipate depressed yang qi,including relieving exterior syndrome with pungent-cool drugs,clearing depressed heat,relieving exterior and interior syndrome,eliminating dampness and relieving depression as well as combination of cold and warm drugs.[Conclusion]LIU Wansu treated fire and heat disease from depression of yang qi,providing new methods in treating fire and heat disease for later generations as well as guiding significance in current clinical work.
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[Objective] To observe the effect of Jiedu Quyu Ziyin decoction on TLR4 signaling pathway in macrophages of MRL/lpr lupus mice. [Method] MRL/lpr lupus mice were divided into four groups:model group, prednisone group, Jiedu Quyu Ziyin decoction group(hereinafter referred to as:Chinese medicine group) and prednisone plus Chinese medicine group (hereinafter referred to as:combination of Chinese and western medicine group). The mice were gavaged with saline, prednisone, Jiedu Quyu Ziyin decoction and prednisone added Jiedu Quyu Ziyin decoction for 4 weeks. Macrophages of lung, peritoneal and spleen were collected and the expression of related genes was detected by RT-PCR. [Result] TLR4 mRNA in lung macrophages, and TLR4 protein in splenic macrophages increased significantly(P<0.05) after the treatment of prednisone. The increased TLR4 protein in splenic macrophages was significantly decreased by combining with Jiedu Quyu Ziyin decoction(P<0.05). Prednisone can significantly reduce the TLR4 downstream molecules such as MyD88, IFN-α, iNOS mRNA expression in lung and peritoneal macrophages(P<0.05). The decreased MyD88 mRNA in lung macrophages was increased significantly by combining with Jiedu Quyu Ziyin decoction(P<0.05). [Conclusion] TLR4 signaling pathway is changed in macrophages after glucocorticoid administration in MRL/lpr lupus mice. Jiedu Quyu Ziyin decoction can reduce the abnormal glucocorticoid-induced TLR4 protein expression.
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Objective: To explore the clinical value of four-dimensional ultrasound imaging in obstetric examination. Methods:Four-dimensional ultrasound was detected by V730 on 60 pregnant women who used ultrasonography in prenatal screening. Results:There were 60 cases in which 20 cases with limb malformation, 15 cases with facial deformity, 12 cases with spinal malformation, 8 cases with abdominal malformation, and 5 cases with chest malformation. Conclusion:Four-dimensional ultrasound can better dynamic observation of fetal surface malformations location, which can provide more worthy information for clinical diagnosis and treatment.
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Systemic lupus erythematosus is a diffuse connective tissue disease, which is mediated by autoimmune mechanisms.It results in multiple autoanti-bodies in the serum and injury organs and systems.Modern medicine uses glucocorticoid as the main medicine in treating systemic lupus erythematosus.Glu-cocorticoid is pure yang product, not only wil it injure yin and qi for using long time, but also disturb syndrome type of traditional Chinese Medicine of SLE for using it in large number.In the starting phase of using glucocorticoid, if the patients present manifestation of toxic heat flourishing, we should clear heat and remove toxicity.If the patients present manifestation of yin deficiency and internal heat, we should nourish yin and clear heat.If the patients pre-sent manifestation of stagnated heat stagnation, we should remove toxic heat and blood stasis.In reduction stage of using glucocorticoid, patients present manifestation of qi and yin deficiency.Then we should benefit qi and nourish yin. In maintenance stage of using glucocorticoid, patients present manifesta-tion of yang deficiency of spleen and kidney.So we should warm the kidney and invigorate the spleen.During the whole course of treating, we should also care for blood stasis and toxin.The clinicians should also remove toxic heat and blood stasis depend on the changing of syndrome. Using GCS in treating SLE should depend on the syndrome differentiation and choose the right Traditional Chinese Medicine in order to reach the clinical effect.
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Objective To explore the effects of Jiedu Quyu Ziyin Recipe (JQZR) on the apoptosis and expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte in MRL/lpr mice. Methods 80 MRL/lpr mice were randomly divided into model group,TCM group, Western medicine group and TCM and Western medicine group,20 mice in each group, meanwhile,20 Kunming mice were selected as normal group, then intragastrically administered normal sodium, JQZR apozem, prednisone suspension and JQZR apozem and prednisone suspension, 0. 5ml every time,once daily for 12 weeks respectively. At the end of the 12th week, peripheral-blood lymphocytes of every mice purified by gradient centrifugation were cultivated for 48 hours ,then the apoptosis was detected by flow cytometry. Furthermore,the expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte were detected by RT-PCR. Results At Oh or 48h,the apoptosis ratios of PBLC in normal group, TCM group,Western medicine group and TCM and Western medicine group are higher than model group and the differences are significant(P <0. 05 ,P <0. 01 ) ,while,at Oh,which are not significant within Western medicine group, normal group and TCM and Western medicine group ( P > 0. 05) ,even if which are significant between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group( P < 0. 05 ). At 48h, the differences of apoptosis ratios of PBLC are significant between Western medicine group and TCM and Western medicine group( P < 0. 05 ), and between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group which are significant, too( P < 0. 01 ). The ratios of bcl-2/bax mRNA of normal group,TCM group and TCM and Western medicine group are significantly lower than which of model group or Western medicine group ( P < 0. 05, P < 0. 05, P <0. 01 ,respectively) ,but there is no significant difference between which of model group and Western medicine group.Conclusion JQZR has adjustable effects on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr Mice.
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Objective:To discuss the effect ofChinese herbal medicine ofremoving toxic and blood stasis and nourishing yin on lymphocyte apoptosis in spleen ofMRL/lpr mouse and expression ofCyt-C and Bcl-2.Methods:50 2-month-old male MRL/lpr mouse and 10 2-month-old male C57BL/6 mouse were randomly divided into six groups:high, middle and low dosage groups, pre-treatment group, model group and control group.After 30days treatment, the lymphocyte apoptosis in spleen oflupus mouse and expression ofCyt-C mRNA and Bcl-2 mRNA were detected.Results:The percentages oflymphocyte apoptosis in model group were(57.18?1.72)% lower than that in normal group(78.99?3.76)%(P
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; Objective; To observe the preventive and therapeutic effects of Jieduquyuziyin methods in treating corticosteroid osteoporosis in systemic lupus erythematosus (SLE) and investigate into its mechanism. Method; 147 cases of SLE were randomly divided into the integrated traditional Chinese and western medicine group (79 cases) and the western medicine group (68 cases). There 21 cases which were diagnosed primarily among all 147 cases. 30 healthy cases were taken as the normal control group. The western medicine group (68 cases) was treated mainly with prednisone only, while the integrated traditional Chinese and western medicine group (79 cases) with Jieduquyuyiyin methods on the basis of normal western medicine. Both groups were treated for 3 months as a course. 16 cases in the western medicine group and 25 cases in the integrated traditional Chinese and western medicine group were kept on being treated and observed for 2 years. The lever of serum Ca, P, ALP, BGP, IPTH, 25-(OH) D3 and urinary Ca, P and BMD were checked and observed. Result; The levers of serum P, 25-(OH) D3, BGP and BMD in the normal group, primary diagnosis group, integrated traditional Chinese and western medicine group were higher than those in the western medicine group significantly ( P
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By combining clinical observation and animal experiments and using FASCO - 3010 hemorheological devices to examine the relevant indices, it was found that no obvious changes can be found in the group treated with hormone before and after the treatment, whereas in the group with traditional drugs added, the RBC deformation, and specific viscosity of plasma were all markedly lowered. Comparison of rheological changes in SD rat showed that traditional Chinese drugs can markedly improve the hemorheology in such patients, indicating that these TCM treating principles can improve the concentrated, viscous, coagulated, agglutinated pathological blood status in SLE patients.
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Objective:To study on effects of Chinese herbs for removing toxic substances,nourishing Yin and removing blood stasis on activity of disease in the patient of systemic lupus erythematosus(SLE).Methods:65 cases of SLE were divided into a Chinese medicine group and a control group,and their cumulative scores of SLE disease activity index and changes of doses of prednisone were observed before and after treatment of 2 courses,respectively.Results:At the end of the second treatment course,there was significant difference in the number of case at the active stage and the resting stage between the two groups(P