ABSTRACT
BACKGROUND: It is a challenge to seek for ideal anti-bacterial surface modification by which silver ions can have sustained and durable release. OBJECTIVE: To investigate the in vitro characteristics and effect of titanium dioxide coating with silver ion injection against Staphylococcus aureus . METHODS: There were four groups in the study: control group (pure titanium), titanium oxide coating group, low silver ion group (titanium oxide coating+30 minutes injection of silver ions), and high silver ion group (titanium oxide coating+60 minutes injection of silver ions). Characteristics of the implanted material in each group were observed using scanning electron microscope. Antibacterial efficacy for Staphylococcus aureus was assessed through bacterial instillation method, antibacterial ring test, and microbial growth curve. RESULTS AND CONCLUSION: Silver ions could be loaded onto the surface of the titanium dioxide coating through the silver ion implantation. Moreover, the number of silver ions on the coating surface and the antibacterial ability of the modified coating were both increased with the extension of injection time. Therefore, the silver ion implantation technology is beneficial to prepare inorganic antibacterial materials with strong and long-lasting antibacterial ability.
ABSTRACT
<p><b>OBJECTIVE</b>To develop antibacterial coatings for orthopedic implants with a sustained release of drugs.</p><p><b>METHODS</b>Wollastonite coatings were deposited on the titanium substrates by an atmospheric plasma spray system. After soaking in weight percent of 5% AgNO(3) solution for 24 h, the wollastonite coatings loading silver were obtained. Gentamicin were loaded on the wollastonite coatings by collagen grafting process. The release rates of drugs from wollastonite coatings were investigated by the in vitro solution soaking test. One strain of S. aureus was used in zone of inhibition test to evaluate the antibacterial properties of drug loaded wollastonite coatings, and the cell culture test was used to evaluate their cytotoxicity.</p><p><b>RESULTS</b>Silver and gentamicin loaded wollastonite coatings were successfully prepared. The release of silver ions from the silver loaded wollastonite coatings lasted 50 d in deionized water, effectively inhibiting the growth of S. aureus for 40 d. While an initial burst release of gentamicin was found during the in vitro solution soaking test. The gentamicin released from gentamicin loaded wollastonite coatings can inhibit the growth of S. aureus for 18 d. Both the two kinds of antibacterial wollastonite coatings showed no adverse effect on cellular adhesion, proliferation and alkaline phosphatase expression.</p><p><b>CONCLUSIONS</b>Compared with gentamicin loaded wollastonite coatings, silver loaded wollastonite coatings may have more promising clinical applications due to the even and long-time antibacterial agent release.</p>
Subject(s)
Anti-Bacterial Agents , Pharmacology , Calcium Compounds , Cells, Cultured , Coated Materials, Biocompatible , Pharmacology , Gentamicins , Pharmacology , Materials Testing , Microbial Sensitivity Tests , Osteoblasts , Cell Biology , Silicates , Silver , PharmacologyABSTRACT
Implant-related infection is a commonly occurred complication in orthopaedics. The surface modification methods frequently used for anti-infection of implant surface were minimizing bacteria adhesion,grafting antibacterial agents and improving osteointegration. The pathogenesis and characteristics of orthopaedic implant-related infection and the research advances in the surface modification of orthopaedic implants for anti-infection are described in this paper,providing a reference for the research of novel implants with anti-infection characteristics.