ABSTRACT
<p><b>AIM</b>To search for a new method of left ventricular intubation in rat.</p><p><b>METHODS</b>The wire of percutaneous transluminal coronary angioplasty (PTCA) was put into carotid artery through external carotid artery. Then supported by guide wire, the PE50 tube was advanced into left ventricle.</p><p><b>RESULTS</b>Left ventricular intubation with PTCA guide wire could be performed in 30 rats and successfully repeated in 27 animals.</p><p><b>CONCLUSION</b>The new left ventricular intubation technology in rats is simple and provides a reproducible method.</p>
Subject(s)
Animals , Male , Rats , Anesthesia , Methods , Heart Ventricles , Hemodynamics , Physiology , Intubation , Methods , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of COX1 and COX2 on angiogenesis and endothelial progenitor cell mobilization in rats with experimental myocardial infarction (MI).</p><p><b>METHODS</b>The rats were randomly divided into 3 groups: MI group, MI plus rofecoxib group and MI plus valeryl salicylate group. At the 7th day after operation, circulating EPCs, plasma VEGF and HIF-1alpha mRNA of ischemic myocardium were measured. At the 28th day post operation, capillary densities were also measured in ischemic myocardium.</p><p><b>RESULT</b>Compared with the MI group and the MI plus valeryl salicylate group, circulating EPCs, plasma VEGF, HIF-1alpha mRNA and capillary densities of ischemic myocardium were all decreased in MI plus rofecoxib group.</p><p><b>CONCLUSION</b>The present study revealed that COX2 play an important role with angiogenesis and endothelial progenitor cell mobilization in rat with experimental MI by modulating expression of VEGF and HIF-1alpha.</p>
Subject(s)
Animals , Female , Rats , Cyclooxygenase 2 , Cyclooxygenase Inhibitors , Pharmacology , Endothelial Cells , Cell Biology , Hypoxia-Inducible Factor 1, alpha Subunit , Myocardial Infarction , Drug Therapy , Pathology , Neovascularization, Physiologic , Random Allocation , Rats, Sprague-Dawley , Stem Cells , Metabolism , Vascular Endothelial Growth Factor A , BloodABSTRACT
Background Stromal cell-derived factor-1_?(SDF-1_?)has been demonstrated to be essential for stern cell mobilization/homing.Recent evidence indicates that SDF-1_? has been expressed in injured carotid arter- ies.Besides,high SDF-1_? plasma levels are clinically associated with stable coronary artery disease.Objective To investigate whether SDF 1 involves in mobilization of endothelial progenitor cells(EPC)and reendothelialization after vascular injury.Methods SDF-1_? was detected by RT-PCR and Western blot in carotid arteries of mice at different time points after wire-induced injury.SDF-1_? determination in peripheral blood samples and BM was per- formed by SDF-1_? enzyme-linked immunosorbent assay(ELISA)kit.EPC in peripheral blood collected at different time points after vascular injury were quantified by flow cytornetry.In subgroup,blocking SDF-1 rnonoclonal anti- body was injected,peripheral blood EPC were quantified after vascular injury and reendothelialization of injured ar- teries was determined 14 days later.Results Expression of SDF-1_? was evident at day 1,and peaked at day 3 after arterial injury.A rise in plasmatic concentration of SDF-1_? and a significant reduction of SDF-1_? in bone marrow concentration was noticed at all time points following injury.The amount of circulating EPC was increased shortly after induction of vascular injury and persisted up to 7 days(P