ABSTRACT
<p><b>OBJECTIVE</b>To study the scientific evidence of the traditional preparation of Dachengqi: "Boiling Aurantii Immaturus and Magnoliae Officinalis first, and then adding Rhei to decoct together. Discarding the dregs, adding Natrii Sulfas into the decoction and drinking the upper solution when the Natrii Sulfas has dissolved completely".</p><p><b>METHOD</b>The concentrations of free and combined anthraquinonoids(emodin, rhein, chrysophanol, physcion) in different decoctions were determined with HPLC method respectively.</p><p><b>RESULT</b>When Natrii Sulfas, Aurantii Immaturus and Magnolias Officinalis are decocted with Rhei in different schemes, the concentrations of anthraquinonoids were changed regularly.</p><p><b>CONCLUSION</b>The scientific evidence of traditional preparation method greatly increased the concentrations of the active components in Dachengqi.</p>
Subject(s)
Anthraquinones , Citrus , Chemistry , Drug Combinations , Drugs, Chinese Herbal , Chemistry , Emodin , Hot Temperature , Magnolia , Chemistry , Materia Medica , Chemistry , Plant Extracts , Chemistry , Plants, Medicinal , Chemistry , Rheum , Chemistry , Sulfates , Time FactorsABSTRACT
To determine the content of isopsoralen in Gushuling Capsule. High performance liquid chromatography was performed on C 18 column. The chromatographic conditions were as follows: methanol-water (48∶52) as mobile phase, flow rate being 1.1?mL/min and the detecting wavelength at 245?nm. A good linearity of isopsoralen was shown in the range of 0.0342～0.2736??g, r=0.9998. The average recovery is 92.11%, RSD=1.89% (n=5). The method is with good reproducibility,RSD=2.05%.[Conclusion]This method can supply evidence for the quality control of Gushuling Capsule.
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Objective To study the quality control standard of Gushuling capsule. Methods Fructus Psoraleae and Fructus Ligustri Lucidi in Gushuling capsule were identified by TLC; The content of ieariin was determined by HPLC with acetonitrile-water (30: 70) as mobile phase and the wavelength at 270 nm. Results TLC identification was positive; the linear range of icariin was 0.0968～0.4840 ?g(r=0.9999), the average recovery was 97.27%(RSD=1.88%, n=5). Conclusion This method can be used as a standard of the quality control for Gushuling capsule.
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Objective To optimize the extraction process of neohesperidin in Fructus Aurantii Immaturus.Methods The optimum proess was investigated by L9(34) orthogonal design with the content of neohesperidin as the observation index.Results Extraction times were the main factors for neohesperidin extraction.The optimal process condition was as follows:adding 6-fold 70 %ethanol,extracting for 3 times,one hour for each extraction.Conclusion The optimal extraction technology of neohesperidin in Fructus Aurantii Immaturus is stable and reliable.
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Objective To establish the standard for the quality control of Compound Huangqin Tablets. Methods The identification of Radix Scutellariae,Rhizoma Polygoni Cuspidati and Caulis Mahoniae in compound Huangqin tablets were carried out by TLC.The content of baicalin in the preparation was determined by HPLC.Chromatocolumn was adopted,methanol-0.4 %H3PO4(50 ∶50) used as the mobile phase,flowrate 1.4mL/min,column temperature at 40 ℃and detection wavelength 278 nm . Results The TLC spots were highly clear without the interference of negative control and were reproductive. The calibration curve for baicalin was linear (r=0.9996) in the range of 0.12～0.72 ?g and the mean recovery was 97.17 %and RSD=1.78 %(n=5). Conclusion This method can be used for the quality control of Compound Huangqin Tablets.
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Objective To establish a HPLC method for the determination of psoralen and bergapten in Ficus hirta Vahl.Methods A Merck-lichrospher C18(4 mm?250 mm,5 ?m)column was adopted.The mobile phase was acetonitrile-water(35∶65) with the flow rate of 1.0 mL/min.The column temperature was 35 ℃,and the detection wavelength was set at 222 nm.Results Psoralen's linearity was obtained in the range of 0.12 ?g～1.20 ?g(r=0.999 7),and bergapten's linearity in the range of 0.03 ?g～0.30 ?g(r=0.999 5).Psoralen's average recovery was 100.9 %with RSD 2.9 %,and that of bergapten was 99.6 %with RSD 1.9 %.Conclusion This is the first report of simultaneous determination of psoralen and bergapten in Ficus hirta Vahl.by HPLC,and the results showed the method is accurate,reproducible,and can serve as quality evaluation method for Ficus hirta Vahl.
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Objective To study the different extraction condition on naringin content in Drynaria extract.Methods The Naringin content in Drynaria extract was determined by HPLC.The e ffect of extraction solutions,drying temperature(60℃,100℃and 120℃)and drying time (0,0.5,1.0,1.5,2.0,5.0,10.0,25.0,50.0hours respectively)on naringin content in Drynaria extract was investigated.Results The optimal extraction solution for Drynaria extract was water,and the loss of naringin enhan ced with the increase of drying temperature and the prolongation of dryin g time.Con-clusion The result can provide references fo r the extraction technology,preparation selection and quality control of the compound including Rhizoma Drynariae.