ABSTRACT
Objective To explore a safe and efficient method for endotracheal intubation in mice.Methods A small longitudinal incision was made in the middle of the neck in rats.After the trachea was exposed,the rats were undergone endotracheal intubation and the operation was completed in about 1 min.Results The success rate of intubation was 100% in over 1300 cases.Conclusion The whole procedure can be finished by one person without extra equipment and special mouse position.This novel and efficient method can significantly improve the success rate in using mice as animal models for in vivo studies.
ABSTRACT
With 58 of New Zeeland rabbits, following experiments were carried out: (1) the dose-response relationship of MSG which decreased the body temperat ure; (2) the effects of the propranolol and the anisodamine (654-2) on the action of decreasing body temperature by MSG. The results indicated: following intravenous administration of MSG at a dose of 0.5g/kg. w and 1.0g/kg. w, the body temperature of rabbits were decreased significantly, but at a dose of 0.1g/kg.w, the change of temperature was insignificant.Pretreatment with propranolol did not affect the effect of MSG decreasing body temperature which was blocked significantly by anisodamine, one of blocker of the cholinergic nerve. The authors suggest that the effect of MSG decreasing body temperature may depend on the cholinergic nerve.