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Objective:To explore the asymmetry of neonatal brain white matter using fractional anisotropy (FA) parameter generated from diffusion tensor imaging (DTI).Methods:From January 2011 to June 2013, 88 neonates with normal cranial MRI performance, aged 1-14 (7.7±0.3) days and gestational age 31-42 (37.5±0.3) weeks, were retrospectively collected from the First Affiliated Hospital of Xi′an Jiaotong University. According to the gestational age at birth, the neonates were divided into preterm group (37 cases) and term group (51 cases). DTI was processed to generate FA parametric maps. Tract-based spatial statistics (TBSS) was used to analyze the asymmetry in neonatal white matter. Regions of interest were placed in bilaterally symmetrical white matter tracts. The white matter tracts included the bilateral anterior thalamic radiation, corticospinal tract-posterior limb of the inner capsule (CST-IC), corticospinal tract-corona radiata, cingulum hippocampus part (CGH), inferior frontal occipital fasciculus (IFOF), inferior longitudinal fasciculus (ILF), superior longitudinal fasciculus (SLF), uncinate fasciculus, superior longitudinal fasciculus temporal part (SLF-temp). The FA value was measured and asymmetry index (AI) was calculated. The independent-sample t test or Mann-Whitney U test was used to compare the AI of each tract between the preterm and term groups. Partial correlation was used to analyze the effects of neonatal gestational age and birth anthropometric measures on the asymmetry of neonate at birth. Results:The results of TBSS showed that the left lateralized areas of brain white matter in the neonatal period were mainly located in the corticospinal tract, external capsule and genu of corpus callosum. The right lateralized areas of brain white matter in the neonatal period were mainly located in the optic radiation and splenium of corpus callosum. In both the preterm and term groups, the tracts related to motor (CST-IC) and language function (ILF, SLF, SLF-temp) were left asymmetry. The CGH of the preterm and the term neonates were right asymmetry, and AI was -0.086±0.114 and -0.140±0.108 respectively, with a statistically significant difference ( t=2.27, P=0.026), while the differences of AI in the remaining tracts were not statistically significant ( P>0.05). Only gestational age was correlated with the AI of ILF ( r=0.234, P=0.033), SLF ( r=0.259, P=0.018), SLF-temp ( r=0.252, P=0.022), CST-IC ( r=0.235, P=0.033). No significant correlation was found between birthweight, head circumference, body length and AI. Conclusions:The asymmetries of brain white matter already exist in neonates aged less than two weeks. The tracts associated with motor and language function are predominantly left asymmetry.
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OBJECTIVE@#To explore the genetic basis for a patient with clinically suspected neurofibromatosis type I, alopecia areata and vitiligo.@*METHODS@#Variant of the NF1 gene was detected by chip capture and high-throughput sequencing. Candidate variant was verified by Sanger sequencing of the family trio.@*RESULTS@#The patient was found to harbor a novel missense c.1885G>A (p.Gly629Arg) variant of the NF1 gene, for which neither parent was carrier. The variant was not recorded in the public database. Based on the guidelines for genetic variation of the American College of Medical Genetics and Genomics, the c.1885G>A missense variant was predicted to be pathogenic (PS1+PS2+PM2+PP3+PP4).@*CONCLUSION@#The c.1885G>A missense variant probably underlay the disease in this child. Above finding has enriched the spectrum of the NF1 gene variants.
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Child , Humans , Alopecia Areata/genetics , Genomics , Mutation , Neurofibromatosis 1/genetics , Vitiligo/geneticsABSTRACT
Objective@#In order to provide guidance for the prevention and control of epidemic situations in schools, this paper explores the risk perception and prevention behaviors of college students after classes resume in the post-epidemic period of novel coronavirus pneumonia.@*Methods@#By using convenient sampling method, 835 college students who resumed classes were randomly selected from a university in Guizhou. Self-efficacy questionnaire, perceived social support questionnaire and self-designed questionnaire were adiministered.@*Results@#Totally 37.0% of college students believed that the risk of infection was high, 57.4% of college students believed the severity of infection was very high once get infected, meanwhile, 84.3% of them think that keeping away from crowded places was a better preventive behavior, 89.7% of college students often wear masks, 91.4% of college students wash their hands frequently. There was a significant positive correlation between general self-efficacy, perceived social support and preventive behavior (r=0.10-0.65, P<0.01). General self-efficacy and perceived social support play both a partial intermediary role and a chain intermediary role between risk perception and preventive behavior.@*Conclusion@#Risk perception of Novel Coronavirus was moderate among college students, who can take active measures to effectively prevent it. Risk perception could not only directly affect the prevention behavior of college students, but also indirectly affect the prevention behavior of college students through understanding social support and general self-efficacy.
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The aim of this study is to determine the accuracy of RSI and RFS in the diagnosis of hypopharyngeal reflux （LPR）, the scores of RSI and RFS were compared in different age groups. To explore the RSI and RFS scoring thresholds for diagnosis of LPR in different age groups. From January 2017 to March 2019, 258 patients with suspected LPR in our hospital outpatient clinic completed the RSI and RFS scales. According to their age, 258 patients with RSI>13 and RFS>7 were selected. They were divided into group A （18-0.05）, RSI score was significantly different （<0.05）, RSI score of group A and group B was higher than that of group C （<0.05）. According to ROC analysis, the best RSI cutoffs for diagnosing LPR in group C was 11, and the area under the ROC curve was 0.866. The RSI score is a good criterion for the diagnosis of LPR. The diagnostic threshold of RSI in elderly patients is different from that in young patients. For elderly patients, the diagnostic threshold for the recommended RSI score is 11.
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Objective@#The aim of this study is to determine the accuracy of RSI and RFS in the diagnosis of hypopharyngeal reflux （LPR）, the scores of RSI and RFS were compared in different age groups. To explore the RSI and RFS scoring thresholds for diagnosis of LPR in different age groups. @*Method@#From January 2017 to March 2019, 258 patients with suspected LPR in our hospital outpatient clinic completed the RSI and RFS scales. According to their age, 258 patients with RSI>13 and RFS>7 were selected. They were divided into group A （18-<40 years, n=86）, group B （40-<60 years, n=107） and group C （≥60 years, n=65）. The diagnosis was confirmed by 24 h pH-metry. The diagnostic rate, RSI and RFS scores were compared among the three groups. ROC curve was used to analyze the optimal thresholds for the diagnosis of LPR by RSI and RFS scores. @*Result@#Among the three groups, group C had the highest diagnostic rate（93.85%）. There was no significant difference in RFS score among the three groups （P>0.05）, RSI score was significantly different （P<0.05）, RSI score of group A and group B was higher than that of group C （P<0.05）. According to ROC analysis, the best RSI cutoffs for diagnosing LPR in group C was 11, and the area under the ROC curve was 0.866. @*Conclusion@#The RSI score is a good criterion for the diagnosis of LPR. The diagnostic threshold of RSI in elderly patients is different from that in young patients. For elderly patients, the diagnostic threshold for the recommended RSI score is 11.
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OBJECTIVE To analyze the effect of postaural injection in treating SNHL and the influence on ROS,sVCAM-1 levels.METHODS 242 low frequency descent sensorineural hearing loss patients from Jan.2010 to Jul.2016 in our hospital were selected as the research sbjects.They were randomly divided into two groups(n=121).All patients were treated with ginkgo biloba extract intravenous drip.The control group was treated with Methylprednisolone intravenous drip,while the observation group was treated with postaural injection of Methylprednisolone.The clinical effects between the two groups were compared after 14 days of treatment.The ABR result,serum ROS,sVCAM-1 levels were also copmpared before and after treatment.RESULTS The effective rates of 2 groups were 82.64％,61.16％ respectively,which indicated significant difference (P＜0.05).The Ⅰ and Ⅴ waves of observation group were decreased,and they was significantly lower than that of the control group(P＜0.05),the R-R interval between Ⅰ and Ⅴ waves of the 2 groups showed no significant difference (P＞0.05).After treatment,the serum ROS level of 2 groups were (0.66 ± 0.38) ng/ml,(2.31± 1.12) ng/ml respectively,the sVCAM-1 level of two groups were (230.5 ±26.4) ng/ml,(312.6 ±47.2) ng/ml respectively,the observation group was significantly lower than that of the control group (t=10.985,11.953,P＜0.05).CONCLUSION Methylprednisolone can improve the clinical effect in treating low frequency descent SNHLby improving the concentration of drugs in ear and increasing the activity of Na+/K+-ATP enzyme in blood vessel;It can also decrease ROS and sVCAM-1 level,alleviate oxidative stress,reduce vascular endothelial damage,which is worthy of recommendation.
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OBJECTIVE:To observe the efficacy and safety of different administration of dexamethasone in the treatment of se-cretory otitis media. METHODS:Data of 92 patients with secretory otitis media was retrospectively collected and divided into ob-servation group(43 cases)and control group(49 cases)by different administration. Observation group received 5 mg Dexametha-sone injection by injection in the eustachian tube in the assisted by video laryngoscope,once every 2 day. Control group received 5 mg Dexamethasone injection by injection in the eustachian tube,once every 2 day. 7-day was regarded as 1 treatment course. 1 more course for uncured patients,and no more than 4 courses. Clinical efficacy,and tumor necrosis factor-α(TNF-α),C-reactive protein(CRP),interleukin-6(IL-6),IL-10 levels before and after treatment,and 1-year cumulative recurrence rate after cured and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was signifi-cantly higher than control group,1-year cumulative recurrence rate after cured was significantly lower than control group,the dif-ferences were statistically significant(P0.05). After treatment,TNF-α and IL-6 level in 2 groups were significantly lower than before, and observation group was lower than control group,the differences were statistically significant(P0.05). And there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:The efficacy of dexamethasone by injection in the eustachian tube in the assisted by electron-nasopharyngolaryngoscopy is superior to auripuncture administration,it can reduce re-currence rate,with good safety.
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BACKGROUND:There is a great dispute on the choice of repair materials for ossicular chain damage. OBJECTIVE:To explore the effects of new hydroxyapatite-bone morphogenetic protein ossicular prosthesis versus al ogeneic cartilage ossicular prosthesis in patients with ossicular chain damage. METHODS:Sixty patients with chronic otitis media were equal y assigned into a control group and a test group. Two groups of patients underwent tympanoplasty and ossicular chain reconstruction with al ogeneic cartilage ossicular prosthesis or hydroxyapatite-bone morphogenetic protein ossicular prosthesis, respectively. Twelve months after surgery, fol ow-up results were compared between two groups. RESULTS AND CONCLUSION:Air conduction value and air-bone gap value were both improved significantly in the two groups after surgery (P Subject headings:Tympanoplasty;Otitis Media;Bone Morphogenetic Proteins;Tissue Engineering
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OBJECTIVE@#To investigate the relationship between the immune status of adenoids and secretory otitis media (SOM).@*METHOD@#The adenoids tissue samples of 30 cases of recurrent secretory otitis media (SOM), 17 cases of non-recurrent secretory otitis media and 20 cases of the adenoids hypertrophy without SOM were studied by immunohistochemical method.@*RESULT@#The expression of PCNA, BCL-2, CD4+, CD8+ cells and CD4+/CD8+ in recurrent SOM tissue were 30.85 +/- 1.73, 21.27 +/- 1.25, 41.90 +/- 9.07; 20.45 +/- 7.08 and 2.10 +/- 0.17, respectively, which was remarkably higher than those of non-recurrent SOM, (25.50 +/- 1.66, 14.23 +/- 1.06, 17.40 +/- 6.85, 13.02 +/- 5.88, 1.33 +/- 0.11, respectively) and those of simple adenoid hypertrophy (25.25 +/- 1.75, 14.05 +/- 1.02, 16.30 +/- 8.21, 11.15 +/- 5.71 and 1.39 +/- 0.15, respectively) (P 0.05). In group of simple adenoid hypertrophy, PCNA in the subgroup of the age 3 to 6 was significantly higher than those in the other groups (P < 0.05).@*CONCLUSION@#In adenoid tissues of recurrent SOM patients, the activities of T-lymphocyte subsets cells in hypertrophic status increase, the adenoids enlarge and local immunity enhances. The age and gender have nothing to do with the SOM, but low age is one of the influencing factors of adenoid hypertrophy. Therefore, adenoidectomy for recurrent SOM patients with adenoid hypertrophy should be carried out as early as possible.
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Adolescent , Child , Child, Preschool , Female , Humans , Male , Adenoids , Allergy and Immunology , Metabolism , Pathology , CD8-Positive T-Lymphocytes , Allergy and Immunology , Metabolism , Flow Cytometry , Hypertrophy , Allergy and Immunology , Metabolism , Pathology , Otitis Media with Effusion , Allergy and Immunology , Metabolism , PathologyABSTRACT
OBJECTIVE@#To investigate the relationship between immune status of adenoids and secretory otitis media (SOM).@*METHOD@#The adenoid tissue samples of 72 cases with SOM and 30 cases with adenoid hypertrophy without SOM were studied by immunohistochemical method.@*RESULT@#The PCNA, BCL-2, CD4+, CD8+ and CD4+ / CD8+ in SOM tissue samples were 30.85 +/- 1.73, 21.27 +/- 1.25, 41.90 +/- 9.07, 20.45 +/- 7.08 and 2.10 +/- 0.17, respectively, which were much more than that of tissue samples without SOM (25.25 +/- 1.75, 14.05 +/- 1.02, 16.30 +/- 8.21, 11.15 +/- 5.71, 1.39 +/- 0.15 respectively) (P < 0.01). The expression of CD4+ in T-lymphocyte was obviously higher than that of CD8+.@*CONCLUSION@#In adenoid tissues of SOM patients, the activity of T-lymphocyte subsets are increased,the adenoids are enlarged and local immunity are enhanced. Therefore,adenoidectomy should be applied in SOM patients as early as possible.
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Child , Child, Preschool , Female , Humans , Male , Adenoids , Allergy and Immunology , Metabolism , CD4-Positive T-Lymphocytes , Allergy and Immunology , CD8-Positive T-Lymphocytes , Allergy and Immunology , Otitis Media with Effusion , Allergy and Immunology , Metabolism , Proliferating Cell Nuclear Antigen , Metabolism , Proto-Oncogene Proteins c-bcl-2 , MetabolismABSTRACT
OBJECTIVE@#To investigate the expression of EMS1 and DcR3 in laryngeal carcinoma and analyze the relation of EMS1 and DcR3.@*METHOD@#The expression of EMS1 and DcR3 protein in 41 laryngeal carcinoma fresh samples and 41 para-carcinoma tissues (to cutting margin > 0.5 cm) were measured by flow cytometry, and 15 normal laryngeal mucosa samples were also studied as controls.@*RESULT@#(1) The quantitative and qualitative expression of EMS1 and DcR3 protein in laryngeal carcinoma tissues was obviously higher than those in para-carcinoma and in normal laryngeal mucosa tissues respectively (P < 0.05). There was no significant difference between the expression of para-carcinoma and normal laryngeal mucosa tissues. (2) In laryngeal carcinoma, the expression of EMS1 and DcR3 protein was independent of patients' clinical classification, tumor size, smoking history, patients' age and sex but associated with tumor metastasis, pathological grade and clinical stage. (3) In laryngeal carcinoma, the expression of EMS1 was positively correlated with that of DcR3.@*CONCLUSION@#EMS1 was positively related to DcR3, which might play an important role in the carcinogenesis and development of laryngeal carcinoma by synergic effect.
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Adult , Aged , Female , Humans , Male , Middle Aged , Cortactin , Metabolism , Flow Cytometry , Laryngeal Neoplasms , Metabolism , Pathology , Neoplasm Staging , Receptors, Tumor Necrosis Factor, Member 6b , MetabolismABSTRACT
Objective To explore the effect of the level of 1,25-Dihydroxy Cholecalciferol in 30 cases of children with endogenous hyperphosphatemia. Methods Using fasting blood and OLPUMS AU400, determination of phosphorus by Phosphomolybdate ultraviolet France, determination of serum calcium by methyl thymol blue spectrophotometry (MTB) colorimetric, Determination of 1,25 (OH)2D3 levels in 30 cases of high-phosphorus blood group and 30 cases of normal serum phosphate group by Ydioimmunoassay. Results The phosphorus and calcium phosphate product in hyperphosphatemia group is significantly higher than the normal serum phosphate group. The difference is statistically significant. The calcium is slightly lower than the normal serum phosphate in hyperphosphatemia group, The difference is not significant ( P > 0. 05 ). The 1,25 (OH)2D3 of hyperphosphatemia group is significantly lower than the normal serum phosphate group. The difference ia statistically significant( P < 0. 01 ). Conclusion Hyperphosphatemia can inhibit the synthesis of 1,25 (OH)2D3.
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This paper introduces the development and the key technology of image guided Surgery Systems (IGSS) and analyses its prospect in this paper. IGSS can be used in clinical surgery as an assistant tool, and it would be an advanced medical equipment combined with medical robotics.
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Robotics , Surgery, Computer-Assisted , MethodsABSTRACT
Objective To study the relationship between B-type natriuretic peptide and Tei index in elderly patients with heart failure(HF). Methods The plasma BNP levels in elderly patients with HF( n = 65 ) and normal control group(n=30) were measured;at one time, series of ultrasonic parameters were measured by the color Doppler ultrasound,counting up Tei index. Results The plasma BNP levels and Tei index in elderly patients with HF were significantly higher than those in control group (P＜0. 01 ), with NYHA cardiac function classification aggravating,they were significantly higher by levels(P＜0. 01 ) ; the plasma BNP levels were positively related to Tei index(r=0. 517, P＜0. 05). Conclusion Plasma BNP levels and Tei index can be used as clinical indicators to determine the serious degree of elderly HF, and they have good correlation.
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Objective To establish the animal model of gestational diabetes mellitus (GDM to observe the long-term effect of maternal hyperglycemia and postnatal overfeeding on the metabolism of offsprings. Methods Streptozotocin (STZ, 50 mg/kg) was administered to Wistar rats intraperitoneally on the 5th day of gestation to induce animal GDM model. Offsprings were allocated into 3 groups: Group CP (n=8) were exposed to normal maternal hyperglycemic environment, group DP-1 (n=8) exposed to intrauterine hyperglycemia and every 8 pups breast-fed by one GMD mother and group DP-2 exposed to intrauterine hyperglycemia and every 4 pups breast-fed by one GDM mother (n=8). Weight gain between 3-10 weeks were recorded, OGTT at 10 weeks and hyperinsulinemia-euglycemia clamp technique was performed, and plasma insulin level was measured by ELISA at 11 weeks. Results Offsprings in DP-1 and DP-2 group had greater weight gain than group CP at 3-10 weeks after birth [(6.68±1.70)% and (6.03±1.85)% vs (4. 74±0.93)%, P< 0. 05], but no difference was found among the three groups in OGTT results (P> 0. 05). The baseline plasma insulin level was lower in the DP-1 group than in the DP-2 and CP group [(0. 76± 0. 37)μg/L vs (1.13±0. 25)μg/L and (1.06±0. 14)μg/L](P<0. 05). GIR60-120 was higher in DP-1 Offsprings of Wistar rats, exposed to intrauterine hyperglycemia, experience excessive weight gain at 3-10 weeks after birth. Overfeeding in early life expedites the decrease of insulin sensitivity in these offspings.
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Objective To study the effect of intrauterine hyperglycemia on leptin level and offspring development in rats.Methods Female and male adult Wistar rats were mated,streptozotocin (STZ,50 mg/kg) was administered intraperitoneally on 5th day of gestation to induce diabetic model,diabetic pups (DP) were exposed to intrauterine hyperglycemia;control pups(CP) were exposed to controls,which was injected with citrate buffer,8 pups were choosed from each group.Weight gain between 3-10 weeks were recorded.Plasma leptin was detected by enzyme-linked immunosorbent assay (ELISA) when the rats were 11 weeks old,and the expression of leptin receptor in hypothalamus was measured at protein level by histomorphology and mRNA level measured by realtime PCR[expressed with integral absorbance(IA)]in 11weeks in order to discuss the relation of leptin and offspring development.Results The fasting blood glucose level was significantly higher in diabetic mother compared with the controls[(28.3±5.1) mmol/Lvs.(6.3±1.4) mmol/L,P＜0.05].However,there was no difference between the fasting blood glucose level in DP group and CP group[(5.1±0.8) mmol/L vs.(5.3± 0.6) mmol/L,P ＞ 0.05].The growth rate between 3-10 weeks was significantly higher in DP group 649.7% than CP group 479.2%,P ＜0.05.The base insulin level was lower in DP group[(0.76±0.37) μg/L vs.(1.06±0.14)μg/L,P ＜ 0.05];while there was no difference in plasma leptin and the expression of ieptin receptor in hypothalamns[(113±37) μg/L vs.(128±40) μg/L,P ＞0.05].The growth rate was not associated with plasma leptin in DP group (r=-0.501,P=0.311) but associated in CP group (r=-0.553,P=0.001).The protein level of DP group (4125±414) did not significantly differ from that of CP group (4244±511).The median of mRNA of leptin receptor in hypothalamus in DP group did not altered significantly compared with that of CP group(1.25 vs 1.80,P＞0.05).Conclusions Intrauterine hyperglycemia accelerated growth rate of offsprings between 3 and 10 weeks,however,plasma leptin was not discreased,which indicated leptin resistance.Intrauterine hyperglycemia did not influence the expression of leptin receptor in hypopthalamus in offsprings,this suggested the leptin resistance may be not caused by the quantity of leptin receptors.
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In the treatment of 48 cases of peripheral facial paralysis by puncturing Yangbai (GB 14)toward Yuyao (Ex-HN 4), Dicang (ST 4) toward Jiache (ST 6), Xiaguan (ST 7) toward Jiache (ST 6),Sibai (ST 2) toward Quanliao (SI 18), Baihui (GV 20) toward Taiyang (Ex-HN 5), Yintang (Ex-HN 3)toward Shangen (Ex-HN), Cuanzhu (BL 2) toward Jingming (BL 1), and Hegu (LI 4) toward Laogong (PC 8) on the sick side, with other acupoints added in accordance with the symptoms, the results showed cure in 39 cases, remarkable effect in 7 cases, failure in 2 cases, and the curative rate in 81.2% and the effective rate in 95.8%.
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OBJECTIVE:To investigate the effect of Bidouyan oral liquid(BOL) on granulocyte-macrophage colony-stimulating factor(GM-CSF) release,ultrastructure changes of eosinophil and epithelial cell proliferation in cultured tissue of nasal polyps METHODS:Nasal polyp tissue was cultured in medium containing different concentrations of BOL The growth of epithelial cells surrounding tissue block and ultrastructure changes of eosinophil in culture tissue were observed The content of GM-CSF in supernate was detected by ELISA RESULTS:In comparison with control,the neogenetic epithelial cells cultured in medium with BOL were fewer in number The contents of GM-CSF were decreased with the increase of the concentrations of BOL with significant differences(P