Effects of substrates on the indirect immunofluorescence test for pemphigus vulgaris autoantibodies / 대한피부과학회지

BACKGROUND: Substrates play an important role in the effectiveness of the indirect immunofluro-rescence test. The monkey eaophagus has been known to be a more reliable and reproducible substrate than any other animal substrates for detecting pemphigus vulgaris autoantibodies, but it is quite difficult to get the monkey esophagus in Korea, therefore, we tried to find other substrates which could be substituted for it. OBJECTIVE: We studied to select the substrate which can be the most sensitive, to compare the immunofluorescent sensitivity of various animal substrates and fores,cins for detection of pemphigus autoantibodies, and finally to evaluate the effectiveness of cultured hunan keratinocytes as a substrate for indirect immunofluorescerice. METHOD: With foreskin, guinea pigs, cats, rats, dogs, rabbits and cultured human keratinocytes as substrates, an indirect imrnunofluorescence test was performed to detect pemphigus vulgaris autoantibodies in seven sera from the active patients before treatment and diluted serially from 1:10 to 1:1280 RESULT: We could detect pernphigus autoantibodies in all animal subtrates used in this study except rats and obtain good immunofluorescent results with foreskin, guinea pigs, and cultured human keratinocytes. The results were moderate with dogs, cats, rabbits, and poor with rats. CONCLUSION: We confirm thut cultured human keratinocytes can be a good effective substrate. According to effectiveness, subtrates can be divided into three groups. Foreskin, guinea pigs, and cultured human keratinocytes are included in the good effective substrate group, dogs, cats, and rabbits in the modeate group and rats in the poor substrate group, but we can not find any superiority of substrates within each groups.

Clinical spectrum of epidermolysis bullosa acquisita / 대한피부과학회지

The clinical spectruni of epidermolysis bullosa acquisita(EBA) is much broader than originally thought. Although the full extent of the clinical presenation is still being defined, it is now known that EBA include the followings: a non-inflammatory mechanobullous condition equating wit,h classical EBA; an inflammatory vesiculosullous eruption mimicking bullous pernphigoid; and a mucosal-centered disease with sarring similar to cicatricial pemphigoid. Among the nine cases of EBA, aged between 34 to 70 year-old, seen in recent years, three patients had mechanobullous lesions with skin fragilities and scarrings; three patients had inflammatory bullous eruptions, and three other patient had combined features of mechanobullous/inflammatory bullous lesions. Mucous membrane lesions were recognized in sex cases, and the rnos! frequent site of involvement was the oral mucosae. According to observations of these patients episodes of inflammatory bullous eruptions appeared to be present in seven cases and have been considered as early sympoms of the disease. It has been noted, however, that in two cases lesions develop d as an non-inflammatory mechanobullous from thonset. Based on the ability of EBA to mimick bullous pemphigoid or cicatricial pernphogoid and the fact that such cases have perhaps been missed, we feel EBA is more common than past literature has suggested.

Occupational dermatoses in tannery workers / 대한산업의학회지

No abstract available.

Contact Dermatitis from Metal Working Fluid / 대한피부과학회지

Clinical history and dermatological examinations were performed in 51 patients with contact dermatitis due to metal working fluid who were working at the metal industry. Patch tests were also performed with standard series and oil cooling fluid series. Chronic lichenified eczema such as lichenified and fissured was the most common clinical type(45.1%). 19 patients had positive reaction to either one or more of the standard series and oil & cooling fluid series. We found ammoniated mercury chloride was the most common sensitizer in standard series, followed by potassium dichromate, 4-phenylendiamine dihydrochloride, cobalt chloride, nickel sulfate, quinoline mix and cabra mix. Microbiocide was the most common allergen(4 of 6 positive result in oil & cooling fluid series.

A Case of Lupus Vulgaris Following BCG Vaccination / 대한피부과학회지

We report a case of lupus vulgaris following BCG vaccination in a 12-year-old male. The patient was vaccinated against tuberculosis on right shoulder 6 months ago. The erythemstous papular eruptions began to develop on the BCG vaccinated site 2 weeks after vaccination. The eruptions progressed to be pruritic, erythematous plaque which was flat, crescent shaped, and centrally covered by adherent scales for 6 months. Histopathological findings revealed tubercles and tuberculoid structures composed of epitheloid cells and Langhans giant, cells in the dermis. Caseation necrosis within the tubercles was slight or absent. In addition, there was an infiltrate of mononuclear cells around the tubercles. The skin lesions cleared after 6 months of treatment with oral isoniazid 300mg daily.

A combined regimen of etretinate and UVB modulate T6+ and HLA-DR+ epidermal cells

Using anti-T6 and anti-HLA-DR monoclonal antibodies, this study was designed to attain what would do to epidermal Langerhans cell (LC) markers in psoriatic patients when two remedies, etretinate and UVB, having controversial effects on LC were put to use simultaneously. In normal and psoriatic subjects, HLA-DR+ LC was approximately 80% of T6+ LC and a single dose of UVB equivalent to minimal erythema dose (MED), reduced LC membrane markers to approximately 30% of non-irradiated control. The recovery of LC membrane markers, after a single dose of UVB exposure were significantly faster in the group of etretinate treated psoriatic subject than only UVB irradiated psoriatic control. Taken together, seemed to exert prompt recovery of normalization of the number of LC from the depletion following the UVB.

A case of Psoriasis with Generalized Pustules Treated with Tetracycline / 대한피부과학회지

Four clinical patterns of generalized pustular phase were apparent and have been named the Zumbusch, annular, localized and exanthematic types. Other reported 10 types of pustular psoriasis including the poriasis with generalized pustules. We report a case of psoriasis with generalized pustules in a 18-year-old women, who has numerous scattered pustules with mild fever and leukocytosis. She was treated sucessfully with tetracycline 2.0 gm a day for 4 wks.

A Case of Cysticercosis Treated with Praziquantel / 대한피부과학회지

We have recently experienced a case of cysticercosis treated with praziquantel. The patient, 22-year-old female, with 43 cystic masses had marked clinical response to the administration of praziquantel (75 mg/kg/day) for 7 days. It seems that praziquantel, anticestodal agent, might be a safe and effective therapy for this infection.

A Case of Chronic Granulomatous Infection of Fusarium Solani / 대한피부과학회지

A case of chronic granulomatous infection of Fsarium solani had decreased response to recalled antigens and lacked of dinitrochlorobenzene(DNCB) sensitization in vivo. He has had frequent attacks of common cold-like symptoms and same attacks were very suggestive of pneumonia but he relatively healthy until he developed cutaneous Fusarium infection. Recently he also developed herpes zoster.

A Case of Erythema Elevatum Diutimun / 대한피부과학회지

No abstract available.

A Case of Tinea Capitis Due to Microsporum gypseum / 대한피부과학회지

Microsportcm gypseum is a geophilic fungi abundant in soil throughout the world. The infections may occur either from animal to man(zoophilic) or from soil to man (geophilic). Patierit of 13-year-old Korean male sought medical attention with skin lesion on scalp which showed scaly patch with broken hairs and a round pilar cyst like nodule. Identification of Microsporum gypseum was made by culture on Sabouraud agar media and microscopic exarnination.

Treatment of Alopecoa Areata with Dinitrochlorobenzene / 대한피부과학회지

This study was designed to investigate the therapeutic effect of dinitrochlorobenzene (DNCB) on alopecia areata. Forty patients with alopecia areata were sensitized by applying 0.1ml of 2% DNCB dissolved in aceton solution on the voiar aspect of left forearm. Patients were subsequently chaIlenged at weekly mtervals with 0. 1% DNCB solution appIied to one side of affected area for periods of 2 to 8 months. The other side of affected area was reserved as a contol site. The results were as follows. 1. In 29 out of 40 patients (72.5%), hair regrowth occurred either excIusiveIy on the treated site (18 cases) or considerably faster and denser on treated site(11 cases)(Table 1) 2. In the majority of cases, the regrowth of hair was observed, within eight weeks of the treatment. 3. The response to DNCB depended on the severity of the aIopecia. The response rate were 80.9% (17/21) in moderate degree (25% bald area) caes and 33% (1/3) in alopecia totalis(Tale 1) 4. The rate of response depended on the duration of the alopecia. Satisfaetory results were obtained in 19 out of 25 patients with a history of less than one year(76%), in 7 out of 10 patients with a history of one to three year(70%) and in 3 out of 5 patients with a history of more than three years (60%) (Table 2) 5. The main side reactions were burning sensation (3 cases), severe eczema (2 cases), folliculitis (2 cases) and cervical lymphadenopathy(7 cases). 6. Liver function test and complete blood cell count were in normal range during the course of treatment in 5 randomly selected patients. 7. Although the treatment of alopecia areata with DNCB still remains to be an experimental trial, this therapy could be substituted for corticosteroids in selected cases.

A Study on Incidence and Genetic Background of Atopic Dermatitis / 대한피부과학회지

In atopic dermatitis, genetic factor undoubtedly involved, but precise mode of tra- nsmission is not yet clear. Since the true incidence and mode af transmission requires a large scale prospective study, they are very difficult to determine. This study was performed in order to elucidate incidence and hereditary backgro- und of atpoic dermatitis in the selected and secluded urban area where relatively an inbred population lived, because of substantially less immigration from other area. Investigation of this study were carried out among infants and children under 6 year old, The results were as follows 1) A total of 516 infants and children under 6 year nld were examined (male 267, female 249) 2) The incidence of atopic dermatitis was ll.2% (58/516). 3) 67. 3% of patients with atopic dermatitis (39/58) had a family history of atopic dermitis, urticaria and asthma. 4) Frequent clinical features of these patients were pruritus (93. 1%), dry or coarse skin (85. SF), and facial and extensor involvement of the skin (82.7%). 5) Molluscum contagisum (9 cases), impetigo(7 cases, strophuius (4 cases), and.. uzticaria (2 cases), were combined. with atopic dermatitis.

A Case of Tuberous Sclerosis with Psoriasis / 대한피부과학회지

A 10-year-old boy had pin-head sized, discrete, yellowish waxy, translucent papules on the face and also, various sized round, circumscribed, erythematous patches with silvery white scales on the trunk. The clinical and histopathologic findgs were compatable with diagnosis of tuberous sclersis associate with psorlasis. There were strong familial history during 3 generation in psoriasis(Fig. 1) Both psoriasis and tuberous sclerosis are heritable skin diseases. Some heritable dermatologic conditions found in tuberous sclerosis including ichthyosis, hypertrichosis and palmar and plantar keratoses but there is no report of tuberous sclerosis associated with psoriasis in published literatures.