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Objective To investigate the expressions and effects of EGR-1, p53, p16, Cyclin D1 in Nasopharyngeal Carcinoma (NPC). Methods Expressions of EGR-1, p53, p16, and Cyclin D1 protein in paraffin-embedded specimens of 29 chronic nasopharyngitis, 45 NPC and 24 lymph-node metastasis were studied by immunohistochemical method (SABC). Results The positive rates of EGR-1, p16, and Cyclin D1 protein expressions in chronic nasopharyngitis were 44.83 %(13/29), 89.66 %(26/29) and 24.14 %(7/29) respectively, but no p53 protein expression. In NPC, the positive expression rates of the four proteins were 22.22 %(10/45), 91.11 %(41/45), 53.33 %(24/45) and 66.67 %(30/45), with significant difference compared with chronic nasopharyngitis respectively; and in lymph nodes metastasis were 16.67 %(4/24), 79.17 %(19/24), 70.83 %(17/24) and 75.00 %(18/24) with significant difference compared with those of chronic nasopharyngitis and NPC respectively except for p16 protein. Spearman correlation analysis showed that there were no significant correlation between the expression of EGR-1 and p53, p16, Cyclin D1 respectively both in NPC and in lymph nodes metastasis. In NPC, the expression of p53 showed a positive correlation with the expression of p16; and in lymph nodes metastasis the expression of Cyclin D1 showed positive correlation with the expressions of p16 and p53. Conclusions The expression of EGR-1 protein was significantly decreased both in NPC and in lymph-node metastasis, which suggested that Egr-1 gene may be a tumor suppressor gene and have an important effect in the pathogenesis and the progression of NPC. The overexpression of p53 and Cyclin D1 also have an important role in NPC, but p16 probably have no biological significance in NPC.
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In order to discuss the methods for the promotion of bilingual teaching in general medical college,we have carried out bilingual teaching of pathology for the undergraduate students of clinical medical department by exactly comprehending the meaning of “bilingual teaching”,training a troop of high-quality bilingual teachers,sensibly selecting and using the original edition of English teaching materials and bilingual assistant teaching materials,as well as experimenting feasible teaching means,the results are successful and the primary experience has been got.
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Objective To investigate the effects of H_2O_2 on growth and proliferation in CNE-2Z cells.Methods To identify the effects of H_2O_2 on the growth and proliferation in CNE-2Z cells,Cell growth curves,MTT assay and plate colony formation rate were used;Meanwhile,Cell cycle distribution,proliferation index and apoptosis analysis were performed by flow cytometry(FCM) in human NPC cells CNE-2Z in vitro,before and after the action of H_2O_2;the protein expressions of Egr-1,p53,p16,Cyclin D1 were detected by Western-Blotting analysis.Results There were no EGR-1 and P16 proteins expressions in CNE-2Z cells by Western-Blotting analysis before the action of H2O2;the growth and proliferation of CNE-2Z cells were inhibited significantly by H_2O_2 compared with the control cells,and the apoptosis cells were increased.The increased expressions of EGR-1,P53,and Cyclin D1 protein were also shown in CNE-2Z cells by means of Western-Blotting analysis after the action of H_2O_2;but no P16 protein expression;The expression of EGR-1 protein was significant correlation with P53 protein expression,but no significant correlation with Cyclin D1 protein expression.Conclusion There was no EGR-1 protein expression in CNE-2Z cells,and EGR-1 protein can be induced expression rapidly and transiently by H_2O_2,which probably have an important!role in the growth inhibition and apoptosis.The promotor of Egr-1 gene also can be induced activation by radiation(ROS,reactive oxygen species),there is an important application in therapy of NPC and other tumors.
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Purpose:To study the expression of uPA, uPAR ,PAI 1 in 3 nasopharyngeal carcinoma cell lines CEN 2Z,CEN 2Z H5,CEN 2Z H5 9,with different ability to metastasize through lymph.Methods:RT PCR was used to detect expression of uPA,uPAR,PAI 1 at levels of mRNA.Results:mRNA expression of uPA,uPAR was the highest in CNE 2Z H5 9,whereas the lowest in CNE 2Z;mRNA expression of PAI 1was detected in CNE 2Z and CNE 2Z H5, but revealed no differences,but not in CNE 2Z H5 9.Conclusions:uPA?uPAR may promote the metastasis and invasion of NPC,whereas PAI 1 maybe inhibit it