Spectrophotometric determination of isoniazid nalidixic acid and flumequine through ternary complex-formation with Cd [II] and rose bengal

Simple, sensitive and accurate spectrophotometric procedures for the determination of isoniazid [INH], nalidixic acid [Nx] and flumequine [Fq] are developed. These are based on the reaction of the above mentioned drugs with cadmium [II] to form cationic complexes, which subsequently associate with negatively charged rose bengal to form ion-association ternary complexes. The formed complexes of Nx and Fq are extracted with chloroform, and that of INH with 30% acetone in chloroform to give pink colored solutions with lambda max at 564 nm for Nx and Fq and lambda max at 555 nm for INH. The proposed procedures are applied over concentration ranges of 2.8-5.6 mcg ml-1 of INH, 40-56 mcg ml-1 of Nx and 56-96 mcg ml-1 of Fq. They are successfully applied to the determination of the three drugs in pure and in dosage forms; the relative standard deviations being less than 2%

Primary hepatocellular carcinoma and aflatoxicosis

The aim of the present study is an attempt to extend the epidemiological data incriminating aflatoxins in the etiology of H.C.C. to a clinical study and its relation to the well-established viral etiology of that tumor. Furthermore, to study the reflection of this non-traditional factor on the clinical and investigatory profile of patients with H.C.C

Systolic left ventricular functions in patients with acute HBV infection

The present study comprised [42] patents with icteric hepatitis [B] viral infection as a test group together with [8] patients with icteric hepatitis viral infection and negative hepatitis B viral markers. Eight normal subject of matched age and sex were included as a control group. The work revealed a definite myocardial impairment of systolic function in patients with icteric hepatitis B viral infection. The incidence of impairment was high in young males with splenomegaly. The degree of impairment was more in patients with high serum bilirubin, speciall those who developed E.C.G. manifestations that indicate myocardial affection in general. The study recommend a further work searching for the association between myocardial impairment and another viral causes of hepatitis e.g. HCV, Epstein-Barr and Cytomegalovirus. Also, to search for the association between the HB "e" antigenemia as a parameter of HBV replication and the degree of myocardial impairment in acute virus hepatitis

Effect of natural honey on serum immunoglobulins and salivary IgA

Honey has been largely used in empirical medicine. The most important function of immunoglobuline is the defensive action against infection. The materials of the present study comprised twenty normal persons, who were assessed clinically and laboratory before and after two weeks after daily consumption of fixed amount of honey [60 gm].Our results has revealed statistically significant IgG rise as it represents the principal and maintained antibody response towards the biological activity in honey. We can attribute the rise of serum IgG to the stimulatory effect of honey on the humoral immune response mediated by pollens. We can expect a more clear rise of IgA with prolonged regimes of honey intake as our results revealed a minimal yet stable statistically insignificant rise in all groups. The observed reduction in salivary IgA can be attributed to the possible bactericidal effect of honey on the bacterial growth in oral cavity and gut

Glucose tolerance curve after a honey load versus matched amount of sugars

The effect of honey on glucose tolerance curve versus matched amounts of glucose and artificial formula was studied in diabetic patients. Results indicate that honey is more tolerable than pure glucose or an artificial formula of honey sugars. This is true in normal subjects and adult onset diabetes, honey could replace sugar as a sweetening agent. Unfortunately this beneficial effect of honey is not found in juvenile onset diabetics

Study for the ascitic fluid in cirrhotic patients for diagnosis of spontaneous bacterial peritonitis [SBP]

The material of this study comprised 52 cirrhotic ascitic patients selected from in-patient medical department Mansoura University Hospital. From our study 9.6% of cases, [5/52] were proved to have S.B.P. and 11.5% of then [6/52] had C.N.N.A. So the overall prevalence might be considered 21.2%. Abdominal pain and generalized tenderness are of a more differential diagnostic importance than fever because they are more common in S.B.P. than in C.N.N.A. while fever lacked this differential diagnostic value. S.B.P. and its variant C.N.N.A. occured more commonly in cases with advanced cirrhosis and ascites. Also, ascitic fluid total and differential leucocytic count, lowered ascitic fluid PH and lower ascitic glucose content and decreased ascitic fluid/blood glucose ratio are specific criteria for S.B.P. Lowered ascitic PH and lowered aacitic fluid/blood glucose ratio are more intimate ascitic fluid parameters for ascitic fluid culture positivity. HBsAg positivity, lowered serum albumin and high serum alkaline phosphatase are additional criteria offered by the results of our study. Although the number of culture positive cases were few, yet they were dominantly gram-negative bacilli [E.Coli] which may suggest an enteric origin. A future research for all previously mentioned data in a rather larger group of cases for a longer time is suggested to make a good chance for assessment of the dominant organism and the most suitable chemotherapeutic agent, on side, and the most propable perpetuating factors for the development of this serious complication with high mortality in cirrhotic patients and to put a good scheme for prophylaxis on the other side

Bilharzial oval patterns in rectal and colonic mucosa for patients with urinary bilharziasis

Mucosal biopsies from the rectum and colon of 25 males of pure urinary bilharziasis and negative stools were examined directly in the fresh state for bilharzial oval pattern by a slide compression technique. The method proved valuable in the diagnosis of bilharzial nature of the disease as a supplement to routine urine and stool examination. It has the additional balue of indentifying the species of bilharzia in the different levels of rectum and colon. This showed that the bilharzial oval pattern in patients with pure urinary bilharziasis is dominatly that of pure haematobium and dominant haematobium at 10 cm level as compared to a dominant pattern of pure mansoni and dominant mansoni at cm level irrespective of the presence of recent or past history of anti-bilharzial therapy and finally we can conclude that occuld mansoniasis is common in our locality and is discovered only by mucosal biopsy particularly colonic biopsy

P-wave changes in bilharzial patients with chronic obstructive pulmonary disease

55 patients with C.O.P.D. including 18 with bilharzial hepatosplenomegaly and 7 patients with bilharzial cor-pulmonale together with 10 normal control individuals were the material of the present study. P.E.F.R. was taken as the criterion of the presence of C.O.P.D. and changes of arterial blood gases as criteria to score the severity of C.O.P.D. P-Wave changes known to score the severity of C.O.P.D. [axis and amplitude] were assessed. The presence of associating hepatosplenic bilharziasis with or without bilharzial cor-pulmonale in patients with C.O.P.D. having matching severity were found to changes the incidence of P-Wave axis and amplitude changes in C.O.P.D. This leads to the conclusion that the P-Wave changes taken as criteria for screening the severity of C.O.P.D. are no longer valid in the presence of associating hepatosplenic bilharziasis with or without bilharzial cor-pulmonale in patients with C.O.P.D

Serum, ascitic fluid and urinary biochemical changes in bilharzial patients with refractory ascites

15 ascites patients with aepatosplenic bilharziasis responding to diuretic therapy were taken as control group. 20 patients with bilharzial hepatosplenic affection and retraciory ascites were the material of the present study. All patients from both groups were subjected to investigations to prove pure bilharzial etiology. In their serum, and serum and ascitic fluid enzymes [GOT, G.P.T., Alkaline phosphatase and L.D.H.], Proteins [Albumin total globulin, Alpha[1], Alpha[2] Beta and Gamma globulin]; glucose, cholestrol, bilirubin [total and direct], B.U.N., creatinine and electrolytes [Phosphorus, calicum, pottassium, sodium and chloride] were assessed. Urinary electrolytes [Phosphorus, calcium, calcium potassium, sodium and chlorid] were also meadured for all. The serum biochemical data of patients with refractory ascites showed that they were in a more advanced stage of hepatocellular damage than patients with non refractory ascitis. The ascitographic picture of biochemical data of both groups showed selective significant differences that can be taken as a parameters to fortell refractoriness of ascites during the follow up of cases in the course of that disease. Selective significant differences in urinary electrolytes of both groups can also be taken as forerunnex to predict refractoriness of ascites. The A/S ratio of different biochemical data in both groups showed selective significant differences that can be taken as a parameters for detection of the impending refractory ascitic stage in the course of bilharzial hepatosplenic affection. The concept of an active role for the peritoneum in ascitogrophic biochemical patterns construction was proposed and discussed