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Chinese Journal of Biotechnology ; (12): 940-944, 2006.
Article in Chinese | WPRIM | ID: wpr-325446

ABSTRACT

The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.


Subject(s)
Bacillus subtilis , Genetics , Carbohydrate Metabolism , Carbohydrates , Chemistry , Hexosyltransferases , Genetics , Lespedeza , Genetics , Metabolism , Plants, Genetically Modified , Reverse Transcriptase Polymerase Chain Reaction , Sodium Chloride , Pharmacology , Solubility , Stress, Physiological , Transformation, Genetic , Transgenes , Genetics
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