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Objective To explore the effects and mechanism of hydrogen sulfide on myocardial ischemia reperfusion in rats. Methods With sodium hydrogen sulfide (NaHS) as a donor of hydrogen sulfide ( H2S), we established myocardial ischemia-reperfusion injury model in rats. The SD rats were randomly divided into control group,myocardial ischemia reperfusion group (I/R group), H2S group,and H2S and glibenclamide (H2S + GLI)group. We monitored the hemodynamics index of rats, including heart rate, arterial pressure, left ventricular pressure. The rate of ventrical arrhythmia was also observed in each group. Results H2 S significantly reduced the ventricular arrhythmia (VA) occurrence (H2S group 66.5% vs I/R group 33.5% (P ＜0.05) and score in myocardial ischemia reperfusion rats (H2S group 2. 6 ±0. 7 vs I/R group 4. 5 ±0. 8(P＜0.05). The KATP channel blocker,glibenclamide,could weaken the antiarrhythmic effects of H2S ( H2S group 2. 6 ±0. 7 vs. H2S + GLI group 4. 0 ± 0. 6, P ＜ 0.05 ). Conclusions H2S has the protective effect against myocardial ischemia reperfusion damage. This function may be associated with the KATP signal transduction pathway in cells.
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OBJECTIVE： To investigate BET for five Chinese medicinal herbs for injection METHODS： By interfering test Suanghuanglian for injection showed no interference with BET， Yinzhihuang for injection had inhibiting action， Salviae mtltiorrhizae composita， Qingkailing and Shengmai for injections revealed enhancing action on BET RESULTS： The interference could be eliminated when the samples were diluted with dextran solution CONCLUSION： Five Chinese medicinal herbs for injection can be examined by BET instead of the pyrogen test method
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OBJECTIVE:To discuss the necessity of skin test for consecutive use of penicillin with different batch numbers or manufacturers.METHODS:The domestic situation of skin test for penicillin use and its relevant requirements were analyzed,the anaphylactogens and the related mechanisms were discussed.RESULT&CONCLUSION:It is not necessary to perform a new skin test for continuous use of penicillin with different batch numbers and produced by different manufacturers.
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OBJECTIVE:To investigate BET for five Chinese medicinal herbs for injection METHODS:By interfering test Suanghuanglian for injection showed no interference with BET,Yinzhihuang for injection had inhibiting action,Salviae mtltiorrhizae composita,Qingkailing and Shengmai for injections revealed enhancing action on BET RESULTS:The interference could be eliminated when the samples were diluted with dextran solution CONCLUSION:Five Chinese medicinal herbs for injection can be examined by BET instead of the pyrogen test method
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OBJECTIVE:To establish a RP-HPLC method to simultaneously determine the contents of piperacillin and taz_obactam METHODS:Using ? Bondapak C18 column as fixed phase,a mixture of methanol and purified water(34 5∶65 5)as mobile phase,and caffeine as internal standard,detecting wavelength:220nm,flow rate:1 0ml/min RESULTS:The linear rangs of piperacillin and tazobactam were 40～200?g/ml and 5 0～25 0?g/ml respectively The average recovery and RSD (n=5) were (100 2?0 61)% for piperacillin and (99 03?0 90)% for tazobactam CONCLUSION:This method is rapid,simple and accurate