ABSTRACT
OBJECTIVE:To investigate the protective effect of Guishu blood-activating capsule on cerebral ischemical reperfusion injury of rats.METHODS:The cerebral embolism model rats were established,the rats were randomly divided into model group,nimodipine positive control group,Guishu blood-activating capsule(high,medium and low dosage groups,re?spectively)group and with another sham operation group established.Each group was administered intragastrically with water or the corresponding drugs,the cortexes and hippocampus tissues of the rats were divided into the injured side and the control side,the contents of aspartate(Asp),glutamate(Glu),malonaldehyde(MDA),lactate dehydrogenase(LD),the tumor necrosis factor?(TNF-?)and the activity of glutathione peroxidase(GSH-Px)were determined,the cerebral infarct size was determined by red tetrazoline staining method and the cerebral water content was determined by oven dry process.RE?SULTS:Compared with the model group,in one or several sub-groups of Guishu group,no marked change was noted in the content of Asp while that of the Glu in the injured side of the hippocampus tissues decreased significantly(P
ABSTRACT
AIM:To investigate the effects and mechanism of Le Er Mai(LEM) on the apoptosis of hippocampus neuronal cells in the anaphase of cerebral ischemic reperfusion injury in rats.METHODS:A rat model of middle cerebral artery occlusion reperfusion(MCAO) was produced with the intraluminal filament.During reperfusion for 30 d after 2 h of ischemia,the TUNEL staining methods were used to detect apoptosis of hippocampus neuronal cells,and immunohistochemical technique were employed to examine the protein expression of Fas,Bax,caspase-3 and caspase-9 in the hippocampial.The gene expressions of fas,bax,caspase-3 and caspase-9 in hippocampial were examined by RT-PCR.RESULTS:After 2 h ischemia and 30 d reperfusion,compared with sham-operated group,TUNEL-positive staining cells and expression levels of Fas,Bax as well as caspase-3 and caspase-9 obviously increased,and the mRNA expressions of fas,bax,caspase-3 and caspase-9 in hippocampial markedly up-regulated in model group.Compared with model group,LEM at dose of 2.00 g/kg or 0.87 g/kg,and flunarizinum significantly reduced apoptosis and decreased the protein expressions of Fas,Bax,caspase-3 and caspase-9 in hippocampial,and down-regulated the mRNA expressions of fas,bax,caspase-3 and caspase-9(P