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ABSTRACT Introduction: The strength training of superior members currently practiced in the university courses does not correspond to the real necessities of the athletes, demanding the development of optimized methods for more expressive performance gains. To create these methods, it is necessary to collect data scientifically to consolidate a solid analysis for improvement. Objective: Analyze upper limbs strength training in table tennis players. Methods: During the experiment, 20 students were randomly divided into groups to perform daily training according to the original table tennis training plan. The experimental group received a schematized protocol improving the existing teaching by adding upper limb strength training. The experiment totaled eight weeks, with one hour of training conducted twice a week. Results: In the experimental group, the attack distance index increased by 25.378%, the one-minute attack swing index increased by 0.585%, the swing index increased by 12.795%, and the technical attack index increased by 11.452%. Conclusion: The improved upper limb strength training method presented in the protocol of this article can optimize athletes' muscle strength, increasing balance and swing ability, positively influencing the technical score and final sports performance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O treinamento de força de membros superiores atualmente praticados nos cursos universitários não correspondem às reais necessidades dos atletas, exigindo o desenvolvimento de métodos otimizados para ganhos de desempenho mais expressivos. Para a confecção desses métodos, é necessário efetuar-se uma coleta de dados de maneira científica afim de consolidar uma análise sólida para aprimoramento. Objetivo: Analisar o treinamento de força dos membros superiores nos praticantes de tênis de mesa. Métodos: Durante o experimento, 20 estudantes foram aleatoriamente divididos em um grupo para efetuar o treinamento diário de acordo com o plano original de treinamento do tênis de mesa, enquanto o grupo experimental recebeu um protocolo esquematizado aprimorando o ensino existente, adicionando o treinamento de força para os membros superiores. O experimento totalizou 8 semanas, com uma hora de treino realizado duas vezes por semana. Resultados: No grupo experimental, o índice de distância de ataque aumentou 25,378%, o índice de balanço de ataque de um minuto aumentou 0,585%, o índice de balanço aumentou 12,795% e o índice técnico de ataque aumentou 11,452%. Conclusão: O método aperfeiçoado de treinamento de força dos membros superiores apresentados no protocolo deste artigo pode otimizar a força muscular dos atletas, aumentando a capacidade de equilíbrio e balanço, influenciando positivamente na pontuação técnica e desempenho esportivo final. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El entrenamiento de fuerza de miembros superiores actualmente practicado en los cursos universitarios no corresponde a las necesidades reales de los atletas, exigiendo el desarrollo de métodos optimizados para ganancias de rendimiento más expresivas. Para la confección de estos métodos, es necesario recopilar datos de forma científica con el fin de consolidar un análisis sólido para su mejora. Objetivo: Analizar el entrenamiento de fuerza de los miembros superiores en practicantes de tenis de mesa. Métodos: Durante el experimento, 20 estudiantes se dividieron aleatoriamente en un grupo para realizar un entrenamiento diario según el plan de entrenamiento original de tenis de mesa, mientras que el grupo experimental recibió un protocolo esquematizado que mejoraba la enseñanza existente añadiendo un entrenamiento de fuerza de las extremidades superiores. El experimento duró 8 semanas, con una hora de entrenamiento dos veces por semana. Resultados: En el grupo experimental, el índice de distancia de ataque aumentó un 25,378%, el índice de balanceo de ataque de un minuto aumentó un 0,585%, el índice de balanceo aumentó un 12,795% y el índice de ataque técnico aumentó un 11,452%. Conclusión: El método mejorado de entrenamiento de la fuerza de las extremidades superiores presentado en el protocolo de este artículo puede optimizar la fuerza muscular de los atletas, aumentando el equilibrio y la capacidad de balanceo, influyendo positivamente en la puntuación técnica y en el rendimiento deportivo final. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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@#Objective To summarize the experience of minimally invasive anterior mediastinal tumor resection in our center, and compare the Da Vinci robotic and video-assisted thoracoscopic approaches in the treatment of mediastinal tumor. Methods A retrospective cohort study was conducted to continuously enroll 102 patients who underwent minimally invasive mediastinal tumor resection between September 2014 and November 2019 by the single medical group in our department. They were divided into two groups: a robotic group (n=47, 23 males and 24 females, average age of 52 years) and a thoracoscopic group (n=55, 29 males and 26 females, average age of 53 years). The operation time, intraoperative blood loss, postoperative thoracic drainage volume, postoperative thoracic drainage time, postoperative hospital stay, hospitalization expense and other clinical data of two groups were compared and analyzed. Results All the patients successfully completed the surgery and recovered from hospital, with no perioperative death. Myasthenia gravis occurred in 4 patients of the robotic group and 5 of the thoracoscopic group. The tumor size was 2.5 (0.8-8.7) cm in the robotic group and 3.0 (0.8-7.7) cm in the thoracoscopic group. Operation time was 62 (30-132) min in the robotic group and 60 (29-118) min in the thoracoscopic group. Intraoperative bleeding volume was 20 (2-50) mL in the robotic group and 20 (5-100) mL in the thoracoscopic group. The postoperative drainage volume was 240 (20-14 130) mL in the robotic group and 295 (20-1 070) mL in the thoracoscopic group. The postoperative drainage time was 2 (1-15) days in the robotic group and 2 (1-5) days in the thoracoscopic group. There was no significant difference between the two groups in the above parameters and postoperative complications (P>0.05). The postoperative hospital stay were 3 (2-18) days in the robotic group and 4 (2-14) in the thoracoscopic group (P=0.014). The hospitalization cost was 67 489 (26 486-89 570) yuan in the robotic group and 27 917 (16 817-67 603) yuan in the thoracoscopic group (P=0.000). Conclusion Compared with the video-assisted thoracoscopic surgery, Da Vinci robot-assisted surgery owns the same efficacy and safety in the treatment of mediastinal tumor, with shorter postoperative hospital stay, but higher cost.
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@#The technical combination of artificial intelligence (AI) and thoracic surgery is increasingly close, especially in the field of image recognition and pathology diagnosis. Additionally, robotic surgery, as a representative of high-end technology in minimally invasive surgery is flourishing. What progress has been or will be made in robotic surgery in the era of AI? This article aims to summarize the application status of AI in thoracic surgery and progress in robotic surgery, and looks ahead the future.
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Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-2-4 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p < 0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.
Subject(s)
Bacterial Proteins/metabolism , Lipopeptides/biosynthesis , Bacillus amyloliquefaciens/genetics , Bacillus amyloliquefaciens/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/chemistry , Genome, Bacterial , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , DNA Shuffling , Proteomics , Bacillus amyloliquefaciens/chemistryABSTRACT
Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-24. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-24 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p 0.05) were selected to be excised and analyzed using Matrix-Assisted Laser Desorption/Ionization Time of Flight/Time of Flight Mass Spectrometry, and finally 44 protein spots were confidently identified according to NCBI database. According to clusters of orthologous groups (COG) functional category analysis and related references, the differentially expressed proteins could be classified into several functional categories, including proteins involved in metabolism, energy generation and conversion, DNA replication, transcription, translation, ribosomal structure and biogenesis, cell motility and secretion, signal transduction mechanisms, general function prediction. Of the 44 identified proteins, signaling proteins ComA and Spo0A may positively regulate fengycin synthesis at transcriptional level. Taken together, the present study will be informative for exploring the exact roles of ComA and Spo0A in fengycin synthesis and explaining the molecular mechanism of fengycin synthesis.
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@#Objective To assess the outcome of harmonic scalpel (HS) in esophageal cancer surgery and to provide evidence for the role of HS in the enhanced recovery after surgery (ERAS) and short-term prognosis after esophageal cancer surgery. Methods We collected the clinical data of 81 esophageal cancer patients receiving surgery by HS between January 1 and December 31, 2016 and successfully followed up in our department. There were 67 males and 14 females with an average age of 64.07±7.52 years. Sweet approach was adopted in 16 patients, Ivor-Lewis approach in 23 and McKeown approach in 40. Intrathoracic anastomosis was completed in 41 patients and cervical anastomosis in 40. The length of total hospital stay and postoperative hospital stay, operation time, intraoperative blood loss, duration and volume of thoracic drainage and perioperative complications were recorded and compared in patients with different surgical approaches and anastomosis sites. Results For these 81 patients, the operation time was 276.7±70.4 min, and the intraoperative blood loss was 115.9±69.7 ml. The postoperative duration of thoracic drainage, postoperative complication rate and the drainage volume were 12.3±5.3 d, 35.80% and 1 411.9±1 199.5 ml, respectively. During the follow-up, 15 patients died and the cumulative survival rate was 81.48%. There was no obvious HS-related complication. The operation time of Sweet approach was shorter than that of Ivor-Lewis or McKeown approach (P=0.012) and the dissected lymph nodes were less in Sweet approach (P=0.035). There was no significant difference in perioperative indexes and postoperative short-term prognosis between intrathoracic anastomosis and cervical anastomosis. Conclusion HS is widely used in the surgery for esophageal cancer with safety and effectiveness, which is worthy of clinical application.
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Abstract Purpose: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. Methods: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain. Collagen type III was observed by picric acid sirius red staining under polarized light. The level of insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) was measured by the enzyme-linked immunosorbent assay (ELISA) method. Results: Collagen type III of the H-dose group had a significant difference in histology structure compared with the L-dose group (P<0.05). The maximum load and stiffness decreased significantly in the control group compared with the values of the L-dose and H-dose groups. The stiffness among the three groups differed significantly at the same postoperative time (P<0.05). Interestingly, progressive reestablishment of collagen type III affected tendon-to-bone healing significantly in the later stages. Conclusion: The H-dose was associated with an increased collagen type III morphology stimulated by TGF-β1.