Analysis of incidence and influencing factors of congenital heart disease in perinatal infants in Henan province / 新乡医学院学报

Objective To analyze the incidence, trend and influencing factors of congenital heart disease(CHD) in perinatal infants in Henan province. Methods From 2011 to 2020,1 356 838 perinatal infants born from 28 weeks of pregnancy to 7 days after delivery were selected from 37 national birth defect monitoring points in Henan province as the research subjects, and the incidence of CHD in perinatal infants from different regions, fetal sex and maternal age were compared. The Joinpiont regression model was established to analyze the temporal change trend of the incidence rate of CHD in perinatal infants in Henan province from 2011 to 2020; the annual percentage change (APC) represented the internal trend of each segment, and the average annual percentage change (AAPC) represented the overall change trend. The trend of gap in incidence rate of CHD in different regions, mother's age and perinatal sex was compared by the Joinpiont parallel test. Results From 2011 to 2020,19 004 cases of perinatal infants with CHD were detected in Henan province, with a total incidence of 140.06/10 000; the incidence of CHD in perinatal infants showed an increasing trend year by year (AAPC=43.3% ,P<0.05) From 2011 to 2020, the incidence of CHD in urban perinatal infants in Henan province was significantly higher than that in rural areas, the incidence of CHD in male perinatal infants was significantly higher than that in female perinatal infants，and the incidence of CHD in perinatal infants in older mothers was significantly higher than that in younger mothers (X2=7 259.160,5 415.473,499.520; P<0.05). From 2011 to 2020,the incidence of CHD in perinatal infants in urban and rural areas of Henan province showed an increasing trend year by year (AAPC=42.5% ,44.5% ;P<0.05); the difference between urban and rural areas in the incidence of CHD in perinatal infants was increasing year by year(P<0.05). From 2011 to 2020,the incidence of CHD in male perinatal and famale perinatal infants in Henan province showed an increasing trend year by year(AAPC=44.3%，42.7% ;P<0.05). From 2011 to 2020, the incidence of CHD in perinatal infants of non-elderly and elderly pregnant women in Henan province showed an increasing trend year by year (AAPC=42.9% ,42.7% ;P<0.05).the difference between the elderly arid non-elderly pregnant women in the incidence of CHD In perinatal infants was increasing year by year (P<0.05). Conclusion From 2011 to 2020 ,the incidence of CHD in perinatal infants in Henan province showed an upward trend, and the regional distribution, fetal sex and maternal age were related to the incidence of perinatal CHD.

A case report of huge solitary fibrous tumor of kidney / 中华泌尿外科杂志

Solitary fibrous tumor is a rare mesenchymal tumor associated with NAB2-STAT6 fusion gene, which is rarely seen in kidney. A 16-year-old boy was hospitalized because of left back pain for more than 3 years. Abdominal CT/MRI identified a huge space-occupying lesion in the left kidney. Laparoscopic radical left nephrectomy was performed initially. Nevertheless, laparoscopic-to-open procedure was adopted due to the huge size of the tumor. The pathological diagnosis was renal solitary fibrous tumor. The symptoms of the patient disappeared and no recurrence was observed at the 2-month follow-up after the surgery.

Effects of isorhyncophylline on hippocampal metabolism using 1H NMR-based metabolomics combined with molecular docking analysis / 药学学报

The purpose of this study was to investigate the effect of isorhyncophylline on hippocampal endogenous metabolites in spontaneously hypertensive rats (SHR) by 1H NMR metabolomics and molecular docking. Twelve SHR were randomly divided into a model group and a treatment group. Six Wistar-Kyoto rats were selected as a control group. The rats in the treatment group were administered isorhyncophylline (0.3 mg·kg-1) while the rats in the other two groups were treated with the same amount of sterilized saline solution. Animal experiment was authorized by the Ethics Committee of Shandong University of Traditional Chinese Medicine (No. SDUTCM20210721002). Hippocampal tissues were removed after administration for 8 weeks and assayed by 1H NMR based metabolomics technology combined with a pattern recognition method to find characteristic metabolites, and the metabolic targets were retrieved from the Kyoto Encyclopedia of Genes and Genomes database. Molecular docking technology was used to evaluate binding of isorhyncophylline to the core targets. The results of a principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed a clear cluster of samples among three groups. There were seven differentially altered metabolites, and glucose metabolism and glutamate metabolism were the principal related pathways. Molecular docking indicated that isorhyncophylline had good binding properties with nine key candidate target proteins. According to the above research results, isorhyncophylline can influence energy metabolism and glutamate metabolism in the hippocampus.

Pathogenic role of Chlamydia and Mycoplasma in urinary tract infection and lower urinary tract symptoms / 中华泌尿外科杂志

The pathogenicity of Chlamydia and Mycoplasma in some non sexually transmitted infectious diseases in urology department is unknown. In this paper, the characteristics of related pathogens, detection methods and the related research on their pathogenicity in clinical diseases such as non-specific cystitis, sterile pyuria, lower urinary tract symptoms and upper urinary tract infection are reviewed to guide clinical practice.

Vinpocetine improves the long-term cognitive function of infant rats induced by sevoflurane via Wnt/GSK3β/β-catenin signaling pathway / 中国临床药理学与治疗学

AIM: To explore the effect and mechanism of Vinfluoxine on long-term learning and memory function in infant mice. METHODS: Sixty SD infant rats were randomly divided into Control group, Anesthesia group, Vinpocetine group (5 mg/kg), Vinpocetine group (10 mg/kg), GSK3β inhibition group (10 mg/kg), with 12 infant rats in each group. Two hours before anesthesia, the infant rats in each group were injected with the corresponding drugs. Except for the control group, each group continued to inhale 4% sevoflurane for 4 hours. After 8 weeks, the Morris Water Maze experiments were started. Immunofluorescence double stains were used to detect neuronal proliferation in the brain. Western Blot was used to detect the protein expression in the hippocampus. RESULTS: There was no significant difference in the incubation period of the rats in the early training groups. On the third day of training, compared with the control group, the rats in the anesthesia group reached a significantly increased platform incubation period (P<0.05); In contrast, the intervention of vinpocetine and SB216763 significantly reduced the latency of rats to reach the platform (P<0.05). And in the test, compared with Anesthesia group, the 10 mg/kg vinpocetine and SB216763 intervention rats achieved a significant reduction in the latency of the first time to the platform (P<0.05), and the number of platforms crossing increased significantly (P<0.05). At the same time, sevoflurane anesthesia mediated a significant decrease in the number of BrdU/NeuN positive cells in rats (P<0.05). In addition, sevoflurane mediated a decrease in Wnt3a expression, an increase in p-GSK3β expression, and a decrease in β-catenin expression in the hippocampus (P<0.05). The intervention of vinpocetine and SB216763 significantly reversed the expression of these cells and proteins. CONCLUSION: Vinpocetine improves the long-term learning and memory function of sevoflurane anesthesia-induced infant rats through Wnt/GSK3β/β-catenin signaling pathway.

Simulation on the bragg peak distribution based on SRIM in proton therapy / 中国辐射卫生

Objective To discuss the distribution characteristics of the Bragg peak in proton therapy using the SRIM code. Methods Based on the SRIM code, the transport processes of a high-energy proton beam injecting into different materials (H2O, C2H6O、C8H8、Al and Fe)with incident energies of 50 MeV～250 MeV have been simulated and analyzed. And the relationship between the incident energies, different materials and thickness, and the depth of the Bragg peak was also discussed when the protons injected into different materials and compared with the simulation results of professional Monte Carlo code, such as Fluka 2011 and MCNPX. Results The simulation results indicate that the depths of the Bragg peak increase gradually and the peaks broaden with the increase of incident proton energies for different materials; The ratio of the depth of the Bragg peak in different materials to that in water under the same incident energy changes little and is approximately a constant which doesn’t depend on the proton incident energy. A good consistency was found between the results and those obtained using Fluka and MCNPX programs. Conclusion The simulation accuracy of the SRIM on the proton beam transport is acceptable, and is suitable for the beginning learners.

Correlation between growth characteristics and quality of rhizomes of Notopterygium incisum under wild tending / 中国中药杂志

Study the growth and development process of rhizomes(bamboo-like part) of Notopterygium incisum and the changes of carbohydrate, endogenous hormones and secondary metabolites, and provide theoretical guidance for the formation of high-quality N. incisum medicinal commodities under artificial cultivation. The One-year-old seedlings were transplanted to the original habitat,and the growth and physiological characteristics of N. incisum were dynamically monitored. The results showed that: ① Seedlings transplanted to the original habitat in spring could form rhizomes(bamboo-like part) in the same year. ② After 60 days of transplantation, the root length and root diameter of underground part of N. incisum had increased rapidly, and carbohydrate content in roots and rhizomes had accumulated rapidly. After 120 days of transplantation, the roots and rhizomes of underground part had grown slowly, and starch content in roots and rhizomes increased continuously, while sucrose and total soluble sugar content decreased gradually. ③ The content of abscisic acid(ABA) in rhizomes decreased firstly and then increased, while the indole acetic acid(IAA) content stabilized firstly and then increased rapidly, and the contents of gibberellin(GA_3) and zeatin riboside(ZR) continued to increase. ④ The content of notopterol in rhizomes was higher than that in roots, while the content of isoimperatorin was lower than that in roots, but the total content of the both in rhizomes was higher than that in roots. Therefore, N. incisum can form rhizomes with high content of secondary metabolites under wild tending, and the growth and development of rhizomes are closely related to changes in carbohydrates and are regulated by related endogenous hormones.

Framework construction and standardization practice of standard system for Sichuan Dao-di herbs / 中国中药杂志

Dao-di herbs is one of the characteristics of traditional Chinese medicine different from other ethnic medicine. Sichuan province is rich in varieties and resources of Dao-di herbs, and its development scale and benefits are not obvious in recent decades due to the lag of standards behind the development of the times and objective needs. From the point of view of the whole process and the whole industrial chain, the significance of standardization system for quality assurance, healthy development of the industry, and development of the industry of Sichuan Dao-di herbs are presented in this paper. At present, Sichuan has made every effort to promote the construction and practice of the standardization system for Sichuan Dao-di herbs, to promote the rapid and high-quality development of Dao-di herbs industry in Sichuan.

Clinical characteristics and treatment strategies of patients with fungal infections in the treatment of upper urinary tract calculi / 中华泌尿外科杂志

Objective:To discuss the clinical management, such as characteristics, surgical timing and rational application of antifungal drugs in patients of upper urinary calculi with fungal infections.Methods:A retrospective analysis was performed on 4 patients with fungal infections during the treatment of upper urinary calculi from April 2017 to April 2019. Case 1, male, 55 years old, was admitted to the Department of Nephrology due to febrile urinary tract infection. Right ureteral stone was found during antibacterial treatment. Fever and fungal sepsis occurred after transurethral ureteroscopic lithotripsy. Case 2 Female, 48 years old, frequency and urgency occurred after percutaneous nephrolithotomy of right kidney in another hospital. Urine routine WBCs were full of vision, urine culture was Candida albicans, symptoms disappeared after 2 weeks of oral fluconazole 200 mg QD treatment, urine culture turned negative, discontinued fluconazole symptoms recurred in about 2-4 weeks and the urine culture turned positive, the condition was repeatedly for 1 year. The CT showed multiple small stones in both kidneys. Case 3 Male, 74 years old, frequency, urgency, and dysuria occurred after flexible ureteroscopic holmium laser lithotripsy of left kidney. Urine routine WBCs were full of vision, urine culture was Candida albicans. Symptoms slightly after 2 weeks of oral fluconazole 200 mg QD treatment according to drug sensitivity, but urine culture did not turn negative, discontinued fluconazole symptoms increased. The condition was lasted for one and a half years. His CT showed left kidney lower calyx stones. Case 4 male, 47 years old, frequency, urgency, and dysuria occurred after the surgery of left kidney stone for half a year. Urine routine WBCs were full of vision, urine culture was Candida tropicalis, combined with left kidney cast stones.Results:Case 1, male, 55 years old, was admitted to the Department of Nephrology due to febrile urinary tract infection. Right ureteral stone was found during antibacterial treatment. Fever and fungal sepsis occurred after transurethral ureteroscopic lithotripsy. Case 2 patient was performed bilateral ureteral stent placement for drainage, and two weeks after the oral fluconazole 200 mg QD, she was performed bilateral flexible ureteroscopic lithotripsy, then the urinary fungal infection was cured. Case 3 patient was performed left side ureteral stent placement and amphotericin B and fluconazole antifungal therapy. After his body temperature was normal, he was performed flexible ureteroscopic holmium laser lithotripsy, after the surgery the oral fluconazole 200 mg QD time was just 1 week, resulting in the formation of fungal balls in the left renal pelvis and secondary surgery. Oral fluconazole 200 mg QD combined with fluconazole continuous intraperitoneal perfusion ultimately 1 week cured him after and secondary surgery. Case 4 patient was performed percutaneous nephrostomy drainage and oral fluconazole 200 mg QD for 2 weeks. Then he was performed percutaneous nephrolithotomy lithotripsy, oral fluconazole 200 mg QD was continued until the stent was removed and urine culture turned negative, patient was cured. Case 4 patient had fungal bloodstream infection after ureteroscopic holmium laser lithotripsy. The temperature was normal after intravenous drip of fluconazole 200 mg QD antifungal therapy, and fungal endophthalmitis occurred in ophthalmology 1 week after discharge.Conclusions:Diabetes could be a high risk factor for upper urinary calculi complicated with fungal infection. It is difficult to control the fungal infection without stone removed and it is easy to relapse after surgery. Stones should be removed on the basis of antifungal therapy, and antifungal therapy should be continued after surgery at least 2 weeks after urinary stent removal. If fungal bloodstream infections is diagnosed, eye examination should be done to screen for endophthalmitis to determine if there is tissue dissemination and determine the course of treatment.

Loss of MicroRNA-137 Impairs the Homeostasis of Potassium in Neurons via KCC2

Neuropsychiatric disorders are the leading cause of mental and intellectual disabilities worldwide. Current therapies against neuropsychiatric disorders are very limited, and very little is known about the onset and development of these diseases, and their most effective treatments. MIR137 has been previously identified as a risk gene for the etiology of schizophrenia, bipolar disorder, and autism spectrum disorder. Here we generated a forebrain-specific MIR137 knockout mouse model, and provided evidence that loss of miR-137 resulted in impaired homeostasis of potassium in mouse hippocampal neurons. KCC2, a potassium-chloride co-transporter, was a direct downstream target of miR-137. The KCC2 specific antagonist VU0240551 could balance the current of potassium in miR-137 knockout neurons, and knockdown of KCC2 could ameliorate anxiety-like behavior in MIR137 cKO mice. These data suggest that KCC2 antagonists or knockdown might be beneficial to neuropsychiatric disorders due to the deficiency of miR-137.

Comparison of soil hydraulic properties in wild and cultivated areas of Notopterygium incisum / 中国中药杂志

To clarify the difference of soil moisture characteristics between mixed broad leaf-conifer forest soil and artificial cultivation of Notopterygium incisum, the HYPROP system and the dew point potential meter were used to determine soil water retention curves(SWRC) for samples of two horizons(i.e. 2-7 cm, 10-15 cm). The basic physical and chemical properties of soil and its water characteristic parameters were also determined. The result showed as fllows:①The bulk density of mixed coniferous-broad leaf forest soil was between 0.33 and 0.52 g·cm~(-3), significantly lower than the corresponding value of field soil(1.01-1.18 g·cm~(-3))(P<0.05), While the organic matter content was significantly higher than the corresponding value of field soil(P<0.05). ②The saturated water content(θ_s), field water holding capacity(θ_(FC)) and Water that can be effectively utilized by plants(θ_(PAC)) of mixed coniferous-broadleaved forest soil were significantly higher than the corresponding value of field soil(P<0.05), while the retained water content(θ_r) value that cannot be effectively utilized by plants was significantly lower than that of field soil(P<0.05). ③The values of structural porosity(0.13-0.24 cm~3·cm~(-3)) and Matrix porosity(0.34-0.44 cm~3·cm~(-3)) of mixed coniferous-broadleaved forest soil were higher than the corresponding values of field soil. Therefore, with low bulk density and high content of organic matter, mixed coniferous-broadleaved forest soil can store more water in soil in the form of effective water to meet the needs of plants for water, thus possibly forming high quality medicinal materials of Notopterygii Rhizoma et Radix. In conclusion, the results of this study can provide theoretical basis guidance for soil structure improvement and water management to form high quality medicinal materials in the artificial cultivation of N. incisum.

Ethnobotanical study of Juenang cultural area in Rangtang county of northwestern Sichuan / 中国中药杂志

Indigenous knowledge and traditional culture for sustainable use of native plants in Juenang cultural region of Rangtang county, Aba Zang and Qiang Prefecture of Sichuan province, have been characterized in this paper followed the principles and methods of ethnobotany. The results indicate that 38 species from 27 families(including 6 species of fungi) are ethnobotanically used commonly in this area. Of 38 species of the native plants, 13 species from 12 families are collected for eatables and vegetables, 12 families and 16 species of indigenous plants for medicinal and edible use, 4 species from 4 families for decoration, 4 species from 4 families used for building materials or firewood, and 1 species from 1 families used for religious folklore. Under the influence of Juenang culture and Tibetan culture, indigenous knowledge such as instinctive reverence and gratitude for nature, protection ecological environment and habitats, and moderate use of natural resources(especially wild bioresource), have been gotten passed on from generation to generation in Juenang culture region of Rangtang county, which is of great significance to the protection of local bioresources and environment, including ethnic medicinal plants, and also to provide practical guidance for biodiversity conservation and ecological restoration in those alpine ecological vulnerable areas.

Automated microfluidic chip system for radiosynthesis of PET imaging probes / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Positron emission tomography (PET) is a powerful non-invasive molecular imaging technique for the early detection, characterization, and "real-time" monitoring of disease, and for investigating the efficacy of drugs (Phelps, 2000; Ametamey et al., 2008). The development of molecular probes bearing short-lived positron-emitting radionuclides, such as 18F (half-life 110 min) or 11C (half-life 20 min), is crucial for PET imaging to collect in vivo metabolic information in a time-efficient manner (Deng et al., 2019). In this regard, one of the main challenges is rapid synthesis of radiolabeled probes by introducing the radionuclides into pharmaceuticals as soon as possible before injection for a PET scan. Although many potential PET probes have been discovered, only a handful can satisfy the demand for a highly efficient synthesis procedure that achieves radiolabeling and delivery for imaging within 1-2 radioisotope half-lives. Only a few probes, such as 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG) and [18F]fluorodopa, are routinely produced on a commercial scale for daily clinical diagnosis (Grayson et al., 2018; Carollo et al., 2019).

Association between distribution of bacillary dysentery and meteorological factors in Beijing, 2004-2015 / 中华流行病学杂志

Objective To analyze the distribution characteristics of bacillary dysentery in Beijing during 2004-2015 and evaluate the influence of meteorological factors on the temporal and spatial distribution of bacillary dysentery.Methods The incidence data of bacterial dysentery and meteorological data in Beijing from 2004 to 2015 were collected.Descriptive epidemiological analysis was conducted to study the distribution characteristics of bacterial dysentery.Linear correlation analysis and multiple linear regression analysis were carried out to investigate the relationship between the incidence of bacillary dysentery and average precipitation,average air temperature,sunshine hours,average wind speed,average air pressure,gale and rain days.Results A total of 280 704 cases of bacterial dysentery,including 36 deaths,were reported from 2004 to 2015 in Beijing,the average annual incidence was 130.15/100 000.The annual incidence peak was mainly between May and October,the cases occurred during this period accounted for 80.75％ of the total,and the incidence was highest in age group 0 year.The population distribution showed that most cases were children outside child care settings and students,and the sex ratio of the cases was 1.22 ∶ 1.The reported incidence of bacillary dysentery was positively associated with average precipitation,average air temperature and rain days with the correlation coefficients of 0.931,0.878 and 0.888,but it was negatively associated with the average pressure,the correlation coefficient was-0.820.Multiple linear regression equation for fitting analysis of bacillary dysentery and meteorological factors was Y=3.792 + 0.162X1.Conclusion The reported incidence of bacillary dysentery in Beijing was much higher than national level.The annual incidence peak was during July to August,and the average precipitation was an important meteorological factor influencing the incidence of bacillary dysentery.

Association between distribution of bacillary dysentery and meteorological factors in Beijing, 2004-2015 / 中华流行病学杂志

Objective To analyze the distribution characteristics of bacillary dysentery in Beijing during 2004-2015 and evaluate the influence of meteorological factors on the temporal and spatial distribution of bacillary dysentery.Methods The incidence data of bacterial dysentery and meteorological data in Beijing from 2004 to 2015 were collected.Descriptive epidemiological analysis was conducted to study the distribution characteristics of bacterial dysentery.Linear correlation analysis and multiple linear regression analysis were carried out to investigate the relationship between the incidence of bacillary dysentery and average precipitation,average air temperature,sunshine hours,average wind speed,average air pressure,gale and rain days.Results A total of 280 704 cases of bacterial dysentery,including 36 deaths,were reported from 2004 to 2015 in Beijing,the average annual incidence was 130.15/100 000.The annual incidence peak was mainly between May and October,the cases occurred during this period accounted for 80.75％ of the total,and the incidence was highest in age group 0 year.The population distribution showed that most cases were children outside child care settings and students,and the sex ratio of the cases was 1.22 ∶ 1.The reported incidence of bacillary dysentery was positively associated with average precipitation,average air temperature and rain days with the correlation coefficients of 0.931,0.878 and 0.888,but it was negatively associated with the average pressure,the correlation coefficient was-0.820.Multiple linear regression equation for fitting analysis of bacillary dysentery and meteorological factors was Y=3.792 + 0.162X1.Conclusion The reported incidence of bacillary dysentery in Beijing was much higher than national level.The annual incidence peak was during July to August,and the average precipitation was an important meteorological factor influencing the incidence of bacillary dysentery.

Estimation of reserves of wild Dipsacus chinensis based on MaxEnt and ArcGIS model / 中草药

Objective To study the estimating reserves of wild Dipsacus chinensis and provide a new method for the Chinese materia medica (CMM) resource quantitative evaluation. Methods The major contributing factors for ecological distribution of developing potentiality medicinal plants of D. chinensis were screened on the GIS platform by using the MaxEnt model, and raster data of species density and individual biomass of D. chinensis were generated by MaxEnt model and patial interpolation technology based on the field survey data. Spatial quantitative analysis and assessment of the distribution of resources of D. chinensis was performed by using ArcGIS. On this basis, a reserves assessment model was formatted for sustainable utilization of CMM resources evaluation. Results The analysis showed that the distribution area of D. chinensis was about 3.34 × 105 km2 which was mainly distributed in Hengduan Mountains and the Qinghai Plateau southeast area, and resources reserves was about 3.08 × 107 kg. The results showed that more than 69.48% resources of D. chinensis was located in Sichuan Province, and the western Sichuan such as Aba, Ganzi, and Liangshan prefectures was the highly populated areas. According to that regional center into the surrounding showed a trend of decline, the overall characteristic of individual biomass geographic distribution showed an increased trend from the vertical plane of the Hengduan Mountains as interface to both sides. The results also indicated that seasonal temperature variation coefficient, annual average temperature range, and average rainfall in April were the dominant factors contributing to the underground biomass accumulation. While mean temperature in December, monthly average rainfall in July and August were negative correlated with the underground biomass accumulation. Conclusion This study provides strong data support for the development and utilization of new resources of wild D. chinensis, and helps to establish a new way to the visual quantitative evaluation of CMM resources.

Effects of soil water content on growth and accumulation of active ingredients of Notopterygium incisum / 中国中药杂志

To investigate the adaptive mechanism of Notopterygium incisum to water changes, one-year-old seedlings were used as test materials. The characteristics of growth, physiology, and active ingredients of N. incisum were studied at different soil water content by pot experiments. The result showed that: ①The water content at 100% field capacity is conducive to the growth and accumulation of biomass of N. incisum. The leaf length, petiole length, petiole diameter, root diameter, root length and biomass of underground dry of N. incisum were all significantly increased at the 100% field capacity, and they slightly declined at 70% field capacity. 20% field capacity could not provide the necessary water to growth of N. incisum, all N. incisum withered and died. ②With the extension of moisture treatment time, the relative content of chlorophyll (SPAD) in leaves and the activity of peroxidase (POD) in rhizome showed a trend of increasing firstly, decreasing then; the tendency of activity of superoxide dismutase (SOD) in rhizome increased; the content of malondialdehyde (MDA) in rhizome decreased firstly and increased then;. For the late test, the SPAD value, the activity of POD and SOD in N. incisum at the 40% field capacity were lower than 70% field capacity and 100 field capacity, while the content of MDA in rhizome were higher than 70% field capacity and 100% field capacity. ③ At 70% field capacity, the active ingredients accumulated most in the N. incisum. At the 70% field capacity, the notopterol and isoimperatorinwerehigher than100 field capacity and 40 field capacity. In summary, the soil water content at 70%-100% field capacity would contribute to the growth and accumulation of active ingredients of N. incisum.

Efficacy of general anesthesia combined with ultrasound-guided epidural block for laparoscopic surgery in neonates / 中华麻醉学杂志

Objective To evaluate the efficacy of general anesthesia combined with ultrasoundguided epidural block for laparoscopic surgery in neonates.Methods Sixty American Society of Anesthesiologists physical status Ⅰ or Ⅱ neonates,aged 20-60 days,scheduled for elective radical operation for Hirschsprung's disease,were divided into 2 groups (n=30 each) using a random number table:general anesthesia group (group Ⅰ) and general anesthesia combined with ultrasound-guided epidural block group (group Ⅱ).Anesthesia was induced by inhaling 8％ sevoflurane.Anesthesia was maintained with Ⅳ sufentanil 0.2 μg/kg,cisatracurium 0.1 mg/kg and then with additional sufentanil 0.1 μg/kg and cisatracurium 0.05 mg/kg every hour and 2％-3％ sevoflurane was simultaneously inhaled in group Ⅰ.In group Ⅱ,anesthesia was maintained by inhaling 2％-3％ sevoflurane,epidural block was performed at L1.2 interspace,the catheter was caudally advanced into the epidural space under ultrasound guidance,0.8％ lidocaine was injected at a loading dose of 6 mg/kg,the local anesthetic diffusion in the epidural space was observed,and additional 0.8％ lidocaine 3 mg/kg was given every 30 min.Heart rate and mean arterial pressure were recorded at 5 min before pneumoperitoneum,during pneumoperitoneum,at extubation and after extubation.The extubation time,duration of recovery room stay and development of assisted ventilation after extubation were recorded.Pain was assessed and scored at 5 min before discharge from recovery room.The development of epidural block-related complications was recorded in group Ⅱ.Results Operation was smoothly completed with stable anesthesia in two groups.Compared with group Ⅰ,the mean arterial pressure was significantly decreased during pneumoperitoneum and after extubation,the extubation time and duration of recovery room stay were shortened,and the requirement for assisted ventilation after extubation and pain scores were decreased in group Ⅱ (P＜0.05 or 0.01).No epidural block-related complications were found in group Ⅱ.Conclusion General anesthesia combined with ultrasound-guided epidural block is safe and effective for laparoscopic surgery and is helpful for postoperative recovery in neonates.

Industrialization condition and development strategy of Notopterygii Rhizoma et Radix / 中国中药杂志

Notopterygii Rhizoma et Radix, the underground part of Notopterygium incisum and N. franchetii, is used as a classical traditional Chinese medicine, and as raw materials for 262 Chinese patent drugs production in 694 pharmaceutical factories currently. It plays an important role in the whole Chinese medicine industry with irreplaceable important economic and social values. However, wild resource of was abruptly depleted, and large-scale artificial cultivation has been inapplicable. In this study, Utilization history and the industrialization status of Notopterygii Rhizoma et Radix were summarized. Resource distribution, ecological suitability of Notopterygii Rhizoma et Radix and core technologies for seeds production, seedling breeding, large-scale cultivation has been reported by current studies, and basic conditions are already available for industrialization production of Notopterygii Rhizoma et Radix. However, there still some key technical problems need to be solved in the further research, and some policy dimensions need to be focused on in the coming industrialization cultivation of Notopterygii Rhizoma et Radix.

Effect of silencing MACC1 gene on proliferation and invasion of cervical cancer cells / 中国比较医学杂志

Objective To explore the effect of silencing metastasis-associated in colon cancer 1 ( MACC1 ) gene on proliferation and invasion of cervical cancer Hela cells. Methods siRNA MACC1 and siRNA NC were transfected into cervical cancer Hela cells with liposomes. The expression of MACC1 protein and mRNA was detected by western blot and RT-PCR. Cell viability and invasion ability were measured by MTT and transwell assay, respectively. The expression of cyclin D1, cyclin E matrix metalloproteinase 2 (MMP2), MMP9 and the level of extracellular regulated protein kinases ( ERK) phosphorylation was detected by western blot. Results Compared with siRNA NC, the expression of MACC1 protein and mRNA was down-regulated, cell viability and invasion ability were reduced ( P < 0. 01 ) , cell cycle was arrested at G1 phase (P < 0. 01), the expression of cyclin D1, cyclin E, MMP2, MMP9 and p-ERK1/2 was down-regulated (P< 0. 01). Conclusions siRNA MACC1 can inhibit proliferation and invasion of cervical cancer Hela cells, which migiht be related to down-regulation of p-ERK.